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InvisibleBlimeyGrimey
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Registered: 08/24/05
Posts: 3,799
Loc: Puget Sound
Re: Good pics of panaeolus mycelium [Re: lipa]
    #8543712 - 06/20/08 09:20 AM (15 years, 10 months ago)

Is that a spot of bacterial contam on the top left of the agar pics?

I found a pic of my last plate I posted alittle farther into colonization of the plate.


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Invisiblelipa


Registered: 07/24/07
Posts: 2,684
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Re: Good pics of panaeolus mycelium [Re: BlimeyGrimey]
    #8543763 - 06/20/08 09:33 AM (15 years, 10 months ago)

Yes that is bacteria. Nasty shit! Thanks for the photo.

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InvisibleBlimeyGrimey
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Re: Good pics of panaeolus mycelium [Re: lipa]
    #8544114 - 06/20/08 11:46 AM (15 years, 10 months ago)

Your plates are looking good.


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Invisiblelipa


Registered: 07/24/07
Posts: 2,684
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Re: Good pics of panaeolus mycelium [Re: BlimeyGrimey]
    #8544148 - 06/20/08 11:57 AM (15 years, 10 months ago)

They are suspected to be Pan. tropicalis. You can see pics in my gallery under panaeolus. Workman is going to be working on assuring me of what they are.

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OfflineMycoAu
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Re: Good pics of panaeolus mycelium [Re: lipa]
    #8544293 - 06/20/08 12:41 PM (15 years, 10 months ago)

There is a spot in the top left of your picture (away from the majority of the growth) that appears to be bacterial.

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OfflineJuke Adro
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Re: Good pics of panaeolus mycelium [Re: lipa]
    #8547722 - 06/21/08 02:40 PM (15 years, 10 months ago)

Quote:

lipa said:
Man juke those are some rich plates. What is your recipe.




chocolate malt agar :wink:


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Invisiblelipa


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Re: Good pics of panaeolus mycelium [Re: Juke Adro]
    #8547769 - 06/21/08 03:00 PM (15 years, 10 months ago)

Your kidding right!  lol:grin: Really!

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OfflineJuke Adro
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Re: Good pics of panaeolus mycelium [Re: lipa]
    #8547866 - 06/21/08 03:34 PM (15 years, 10 months ago)

nope not kidding, if it did not have the slimy texture I would eat it, its that good lol :crazy2:


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Invisiblelipa


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Re: Good pics of panaeolus mycelium [Re: Juke Adro]
    #8549020 - 06/21/08 10:19 PM (15 years, 10 months ago)

:grin:Such a funny guy! I'll have to try it. Thanks!

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OfflineCosmicString
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Re: Good pics of panaeolus mycelium [Re: Juke Adro]
    #8549320 - 06/22/08 12:38 AM (15 years, 10 months ago)

Juke Adro,

The blue shroom in your sig is stunning, did you take the photo? Any information about it?

Thanks

CS

And the caterpillar is a little disturbing in that it brings back memories of an old Outer Limits show where the bugs had human like faces.

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Invisiblehappyday
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Re: Good pics of panaeolus mycelium [Re: RogerRabbit]
    #8549785 - 06/22/08 04:11 AM (15 years, 10 months ago)

lol Nice pic RR.  If you stand back from a distance you can see the rim of the petri dish.

RR, We used gentamycin sulf. as antibiotic for years and thought it inhibited germination for P. mex A, while not for cubes and many others.  We attempted this with same prints and syringes (we used both) on each run, and a high quality pc controlled incubator.
We later switched to first germinating P. mex A spores on standard MEA, PYA, etc. and then cleaning it up later on genta s. plates.  I know it's prefered to clean it up initially, but this was our conclusion for P mex A. 

I will try this again to see if it was just a fluke and the spores chosen those days just happened to be of lower quality (i.e. dehydrated, aged).   

Not sure but maybe it was Stamets who said running your mycelium continously on antibiotic could later cause your cultures to be less resistant against bad bacteria and less able to utilize their own defense mechanisms.  That's perhaps the case with all organisms when given excessive amounts of antiotics.

btw Lipa, those two plates in these recent pics look like mycelium to me.  At the beginning(first photos) the bottom two did look like possible bacteria, except the two sectoring plates above them.  Now you can clearly see 3-D.  However it might or might not be a polyculture.  If they are result of spores from outdoors, then I find it hard to believe it's not a mix.

I would run with a clean cut from the plate on your first photos, attempt to fruit on a few different substrates,  and then see what happens. 
Oh, what did the spores resemble under the microscope?

Edited by happyday (06/22/08 04:24 AM)

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Invisiblelipa


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Re: Good pics of panaeolus mycelium [Re: happyday]
    #8550131 - 06/22/08 08:57 AM (15 years, 10 months ago)

I am definite that some of the plates are harboring contaminants. I have transfered a lot of colonies into new plates and isolating for now. They are looking good though. I don't get my new microscope until Tuesday. I am soooo excited! Workman pointed me to a good little setup and the college is going to help me calibrate it so I can take measurements. Ye ha!

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OfflineMycoAu
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Re: Good pics of panaeolus mycelium [Re: lipa]
    #8551269 - 06/22/08 04:50 PM (15 years, 10 months ago)

Juke's pic of the caterpillar is from the movie "Labyrinth".  Great movie.  Villianous vile creature it is.

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OfflineJuke Adro
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Re: Good pics of panaeolus mycelium [Re: CosmicString]
    #8552614 - 06/22/08 11:35 PM (15 years, 10 months ago)

Quote:

CosmicString said:
Juke Adro,

The blue shroom in your sig is stunning, did you take the photo? Any information about it?

Thanks

CS

And the caterpillar is a little disturbing in that it brings back memories of an old Outer Limits show where the bugs had human like faces.




no i did not take the pic :frown: wish i did, the mushroom is a Entoloma hochstetteri


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OfflinepanmanPE91
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Re: Good pics of panaeolus mycelium [Re: RogerRabbit]
    #18074582 - 04/07/13 10:29 PM (11 years, 1 month ago)

in yours or anyones experience
how did colonizing panaeolus substrates smell?
particularly pf tek with manure in mason jars.

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OfflineJuke Adro
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Re: Good pics of panaeolus mycelium [Re: panmanPE91]
    #18074665 - 04/07/13 10:43 PM (11 years, 1 month ago)

Mushroom, Earthy.

In future mate if you can't find your answer by search, just make a new thread :smile:
In mush cult :wink:
This is advanced section and the thread is old as hell.
You will get a lot more answers making a new thread


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