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Offlinefantasy2reality
Growing Anew
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Registered: 02/26/08
Posts: 198
Loc: Hockeytown,USA
Last seen: 14 years, 1 month
LC ready to use, now what?
    #8500431 - 06/08/08 03:27 PM (16 years, 7 months ago)

After numerous attempts to concoct a working LC, I finally did it with great success with
Lipa Yai (sp). So this being my first actual attempt to use a LC I need some pointers from the experienced individuals on here.

I put the LC in the fridge to keep here cool,  3 days later I am getting to my WBS PC'ing.
I just have a few quick questions... I am using wide mouth quart jars, they're about 3/4 of the way full with WBS.

(1) How much LC is needed for each jar? I've read 1-2 cc's. Need confirmation.

(2) Can I replace in fridge after using what I needed from the LC Snapple bottle? I am certain I won't use all of it for the 30 jars of WBS I have.

(3) Should the LC be allowed to come to room temperature before I use in my jars of WBS?

Thanks in advance! Many more successful grows to all :smile:


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Can't seem to get a grip on reality!
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OfflineBubba McMushies
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Registered: 03/11/08
Posts: 595
Loc: USA
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Re: LC ready to use, now what? [Re: fantasy2reality]
    #8500457 - 06/08/08 03:35 PM (16 years, 7 months ago)

1. That will work

2. yes

3. Not needed


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SPAWN CALCULATOR!

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Offlinefantasy2reality
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Registered: 02/26/08
Posts: 198
Loc: Hockeytown,USA
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Re: LC ready to use, now what? [Re: Bubba McMushies]
    #8500943 - 06/08/08 06:19 PM (16 years, 7 months ago)

Good, I'm glad I got an answer, cuz I am going to inject tomorrow morning, and set up in a closet.
The closet has been monitored, and is near 75 degrees consistently. This should suffice, correct?


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OfflineBlargIAmDead
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Re: LC ready to use, now what? [Re: fantasy2reality]
    #8500989 - 06/08/08 06:36 PM (16 years, 7 months ago)

It's fine. And be very sure that the needle and injection port are sterile cause I made an awesome GT LC with a gorgeous test jar, put the LC into the fridge and pulled it out a few days later and sucked up some more mycellium then shot up like 9 more jars and had some beautiful penicillin mold cultures :smile:.

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Offlinefantasy2reality
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Registered: 02/26/08
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Re: LC ready to use, now what? [Re: BlargIAmDead]
    #8501009 - 06/08/08 06:42 PM (16 years, 7 months ago)

Quote:

BlargIAmDead said:
It's fine. And be very sure that the needle and injection port are sterile cause I made an awesome GT LC with a gorgeous test jar, put the LC into the fridge and pulled it out a few days later and sucked up some more mycellium then shot up like 9 more jars and had some beautiful penicillin mold cultures :smile:.




Thanks for the advice. Would hate to waste the rest of what is a good culture.

I think it'll go good. we'll see in a few days.

About how long does it take to show signs of growth when using a LC?


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OfflineBlargIAmDead
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Re: LC ready to use, now what? [Re: fantasy2reality]
    #8501028 - 06/08/08 06:45 PM (16 years, 7 months ago)

Got mine in around 8 days. Some people have said as short as 2. But I'd say a good average is a week or less.

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Offlinefantasy2reality
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Registered: 02/26/08
Posts: 198
Loc: Hockeytown,USA
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Re: LC ready to use, now what? [Re: BlargIAmDead]
    #8501037 - 06/08/08 06:47 PM (16 years, 7 months ago)

I know that when I use multispore syringes, I get signs of growth in about 3-4 days.

I stick with the WBS coffee soak tek, and have no problems with this way. So we'll see what happens after a few days.

Thanks


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Can't seem to get a grip on reality!
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Offlinefantasy2reality
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Registered: 02/26/08
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Re: LC ready to use, now what? [Re: fantasy2reality]
    #8501114 - 06/08/08 07:02 PM (16 years, 7 months ago)

I guess I have one more question to add...

After injecting LC, do I shake the crap out of the jar like with multispore?


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OfflineBubba McMushies
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Re: LC ready to use, now what? [Re: fantasy2reality]
    #8501678 - 06/08/08 09:33 PM (16 years, 7 months ago)

With LC yes you could to help spread the myc.

But with multispore you should NOT shake until the jar is about 20% at least because spores need to germinate and meet up with other spores in order for them to achieve their reproductive state.

If you spread the spores out growth could be delayed since they will first have to find a 'mate' before they start to take over the substrate vigorously.


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Offlinefantasy2reality
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Re: LC ready to use, now what? [Re: Bubba McMushies]
    #8502686 - 06/09/08 06:21 AM (16 years, 7 months ago)

Here is an initial picture after injecting jars with 2 cc's each of Lipa Yai LC.
I only stacked the jars to take this photo shot. The jars are inside a clean closet, about 75 degrees consistently.

I'll keep updating as this project materializes.

Thanks





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Can't seem to get a grip on reality!
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Offlinefantasy2reality
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Registered: 02/26/08
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Loc: Hockeytown,USA
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Re: LC ready to use, now what? [Re: fantasy2reality]
    #8514456 - 06/12/08 08:36 AM (16 years, 7 months ago)

WOW, 2 days after innoculating with Lipa Yai LC. I already see growth.

Is this normal? Will try to take pictures later.


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Invisiblewisp

Registered: 04/13/08
Posts: 5,304
Re: LC ready to use, now what? [Re: fantasy2reality]
    #8514465 - 06/12/08 08:40 AM (16 years, 7 months ago)

Yes it is, because you are innoculating with living mycelium, not spores which need to germinate.

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OfflineNeobean
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Registered: 10/07/01
Posts: 975
Loc: Canada
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Re: LC ready to use, now what? [Re: wisp]
    #8514502 - 06/12/08 09:03 AM (16 years, 7 months ago)

Listen to trip, he be on a rolll....

:rockon:


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If y0u want s0meting gr0wn right, y0u g0tta gr0w it y0urself!!!

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Invisiblewisp

Registered: 04/13/08
Posts: 5,304
Re: LC ready to use, now what? [Re: Neobean]
    #8514507 - 06/12/08 09:06 AM (16 years, 7 months ago)

:lol:

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Offlinefantasy2reality
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Registered: 02/26/08
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Re: LC ready to use, now what? [Re: wisp]
    #8514509 - 06/12/08 09:07 AM (16 years, 7 months ago)

Quote:

tripsis said:
Yes it is, because you are innoculating with living mycelium, not spores which need to germinate.




Yeah man, I am amazed at this... I have used MS before this, and it took like anywhere between 6-8 days to show.... this is just 2 days!!! I am impressed and hooked....Just whipped up a R44 LC and it's showing good signs.


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Invisiblewisp

Registered: 04/13/08
Posts: 5,304
Re: LC ready to use, now what? [Re: fantasy2reality]
    #8514510 - 06/12/08 09:08 AM (16 years, 7 months ago)

Good to hear. Keep us updated.:thumbup:

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OfflineNeobean
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Re: LC ready to use, now what? [Re: fantasy2reality]
    #8514516 - 06/12/08 09:12 AM (16 years, 7 months ago)

Quote:

fantasy2reality said:
Quote:

tripsis said:
Yes it is, because you are innoculating with living mycelium, not spores which need to germinate.




Yeah man, I am amazed at this... I have used MS before this, and it took like anywhere between 6-8 days to show.... this is just 2 days!!! I am impressed and hooked....Just whipped up a R44 LC and it's showing good signs.




Just make sure its contam free, and LC is da bomb :bomb:


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Offlinefantasy2reality
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Registered: 02/26/08
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Re: LC ready to use, now what? [Re: Neobean]
    #8521031 - 06/13/08 06:19 PM (16 years, 7 months ago)

here are some updated pictures.... this is 2 days growth using Lipa Yai LC.
My first time using any type of LC to start off my jars of WBS.
I only stacked the jars to take this photo shot. The jars are inside a clean closet, about 75 degrees consistently.





I was having problems with file size, so these are the only two I could get to post.


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Offlineabitavenger
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Registered: 01/15/08
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Loc: east coast
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Re: LC ready to use, now what? [Re: fantasy2reality]
    #8521051 - 06/13/08 06:23 PM (16 years, 7 months ago)

looks good LC's are the best


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Offlinefantasy2reality
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Re: LC ready to use, now what? [Re: abitavenger]
    #8521363 - 06/13/08 07:52 PM (16 years, 7 months ago)

I think the spread out of the growth is amazing, I'm proud of my handy work! LOL I did a few jars with some B+ multispore the same day, and I have just a few little white spots, but nothing like the LC.


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Shop: Left Coast Kratom Buy Kratom Extract   OlympusMyco.com We’re Not Chasing Unicorns—We’re Building Quality (Olympus Myco Grow Bags)   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   MagicBag.co Certified Organic All-In-One Grow Bags   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Mushroom-Hut Liquid Cultures   North Spore Bulk Substrate   Myyco.com Golden Teacher Liquid Culture For Sale


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