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johnnyboy
Bastard Son

Registered: 04/02/07
Posts: 644
Last seen: 11 years, 9 months
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trich, again?
#8412056 - 05/17/08 06:25 AM (15 years, 8 months ago) |
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this is my third tub that im about to loose to trich and i dont know why. iam sterile in everything i do. i cased it about 4 days ago and went to check on it last night and it had a patch of cobweb looking stuff in one corner so i hit it with H202. then looked at it this morning and it looks like this. IME this is what trich usually looks like before it sporalates and turns green. i feel so beaten right now. i never usually check the ph of my casing i just add the 1 tbs of gypsum and 1 tsp of lime per 1 cp of peat. Could this be why?
i use the cooler method to past. my substrate. menaing i put my sub in 2 gallon ziplocks put them in a cooler and fill the cooler with 190 deg. water and let that sit for a few hours.
and i use rr's method for past. the casing http://www.shroomery.org/forums/showflat.php/Number/5998572#Post5998572
i do do open air spawning but i brush my teeth and wear a mask and gloves. and where else am i supposed to do it?
does it look like the trich started in my casing? is there any way i can figure if the trich is just in my casing or actually in the sub too?
i just dont know where to start to try and figure out what iam doing wrong so i can beat this. do you guys have any ideas?

-------------------- I keep the bible in a pool of blood so that none of it's lies will affect me
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Anno
Experimenter




Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 1 day, 4 hours
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Re: trich, again? [Re: johnnyboy]
#8412061 - 05/17/08 06:35 AM (15 years, 8 months ago) |
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Yes, this is mold. Try sterilizing the casing next time.
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Nature Boy
Stranger than most


Registered: 07/09/07
Posts: 8,244
Loc: Samsara
Last seen: 9 hours, 17 minutes
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Re: trich, again? [Re: johnnyboy]
#8412068 - 05/17/08 06:41 AM (15 years, 8 months ago) |
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Quote:
johnnyboy said: this is my third tub that im about to loose to trich and i dont know why. iam sterile in everything i do.
If what you say is true re your sterile technique, it may be that your grows are being sabotaged by your earlier Trich contamination(s). Those spores, if not caught early and immediately dealt with, can linger on walls, rugs, ceilings, etc., etc.
It might become necessary to do a serious decontamination, washing, and cleansing of your entire grow area, tubs, instruments, jars, etc. N.B.
-------------------- All submitted posts under this user name are works of pure fiction or outright lies. Any information, statement, or assertion contained therein should be considered pure unadulterated bullshit. Note well: Sorry, but I do not answer PM's unless you are a long-time trusted friend. If you have a question, ask it in the appropriate thread.
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johnnyboy
Bastard Son

Registered: 04/02/07
Posts: 644
Last seen: 11 years, 9 months
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Re: trich, again? [Re: Anno]
#8412491 - 05/17/08 11:18 AM (15 years, 8 months ago) |
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Quote:
Anno said: Yes, this is mold. Try sterilizing the casing next time.
by this do you mean pc'n it? how long and at what psi?
does anybody see anything wrong with my past. techniques? im going down to get a thermometer with a probe so i can see just how hot the internal temps get and for how long.
also i lysol the room pretty good and hour or so before i do anything in it wouldnt this kill off any molds that are left in there from the previous grows?
-------------------- I keep the bible in a pool of blood so that none of it's lies will affect me
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Nature Boy
Stranger than most


Registered: 07/09/07
Posts: 8,244
Loc: Samsara
Last seen: 9 hours, 17 minutes
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Re: trich, again? [Re: johnnyboy]
#8412575 - 05/17/08 11:58 AM (15 years, 8 months ago) |
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Quote:
johnnyboy said:
does anybody see anything wrong with my past. techniques? im going down to get a thermometer with a probe so i can see just how hot the internal temps get and for how long.
also i lysol the room pretty good and hour or so before i do anything in it wouldnt this kill off any molds that are left in there from the previous grows?
Not sure what the details of your past techniques consist of, so it is difficult to pinpoint where any problem(s) may lie. Sometimes it is a very simple thing like breathing at the wrong time, or excessive movements or steps, that can produce contamination of one sort or another.
Lysol is a good step for dealing with aerosols, but obviously doesn't help if there is some other sytematic problem that it's not designed to address.
The old addage "If at first you don't succeed, try, try, again" seems to be applicable here. Try changing out the most suspect part of your method for another and see if that helps. Try ONE change at a time. You'll get it!
Good vibes, bro. N.B.
-------------------- All submitted posts under this user name are works of pure fiction or outright lies. Any information, statement, or assertion contained therein should be considered pure unadulterated bullshit. Note well: Sorry, but I do not answer PM's unless you are a long-time trusted friend. If you have a question, ask it in the appropriate thread.
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pazzy
Always Hardcore


Registered: 08/24/07
Posts: 1,360
Last seen: 13 years, 2 months
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You pressure cook casing mixes for an hour at 15 p.s.i.
-------------------- Are you happy for a miracle?
Absolutely no source checks/discussions.
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nolongerinuse


Registered: 05/14/06
Posts: 947
Last seen: 10 years, 1 month
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Re: trich, again? [Re: pazzy]
#8412849 - 05/17/08 01:26 PM (15 years, 8 months ago) |
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I've been having trich and cobweb in my incubation chambers. Today i checked my jars, and its the second set thats got the shit....
So, i just took my entire incubation set up into the shower and cleaned the hell out of it with alcohol and hot water.... hopefully this will help to resolve my problems. I suggest you try to do the same with your tubs.
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 22 days
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The problem isn't in your casing layer, which should be pasteurized.
9 times out of 10 when trich shows up prior to first flush, the mold is coming up from the substrate below, not forming on the casing layer itself.
Your 'pasteurization method' for your substrate is not sufficient. I doubt the inside of the bags ever gets above 120F using that procedure. Pasteurize your substrate the same way you're pasteurizing the casing and you'll be fine. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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johnnyboy
Bastard Son

Registered: 04/02/07
Posts: 644
Last seen: 11 years, 9 months
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Quote:
new2grow said: I've been having trich and cobweb in my incubation chambers. Today i checked my jars, and its the second set thats got the shit....
So, i just took my entire incubation set up into the shower and cleaned the hell out of it with alcohol and hot water.... hopefully this will help to resolve my problems. I suggest you try to do the same with your tubs.
i never get trich in my jars. my inoculation procedure is A+. I always wipe down my tubs good with alcohol before i use them so im sure my problem is what RR suggested that my temps arent reaching the props height.
its sort of a given because i switched to this way of pasteurizing 3 tubs ago just because of how fast and easy it was. i thought for sure the temps would of been where i needed them but apparently not. i used to do the jar method for all of my pasteurization but was looking for something a bit more quicker. it just goes to show faster and easier is not always the best.
thanks for everybody's responses.
-------------------- I keep the bible in a pool of blood so that none of it's lies will affect me
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