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Juke Adro
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Registered: 04/05/08
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h202 discovery (NEW HIGH RES PICS UPDATED) (NEW UPDATE 17-6-08)
#8410381 - 05/16/08 05:46 PM (16 years, 6 days ago) |
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i was cleaning my incubator and spilled some h202 in there and forgot to wipe it up, i put all my gear back in then 2 days later i checked my incubator to see how things were going as im impatient, my fingers started to burn slightly and went white as they do when one touches h202 and that was when i realized what had happened .....my jars and tubs looked all 100% colonized but had a blue tinge to them all on the surface and the h202 was being evaporated and made the whole chamber humid.
now the funny part 2 days before i had the spill things were only 50% colonized and 2 days later it was all 90% to 100% which made me believe it's something worth posting what i think happened that it added oxygen to the chamber and the growth just took off and grew so fast i shit my self, it is not a contam as i have some pinning already, and i believe the blue tinge to the top of the myc is just where it got touched by h202
i tested this again to see what would happen i made up a new incubator put 4 50% colonized jars and 3 fresh casings in the chamber added 50mls of h202 and incubated at 25c for 3 days agian the myc took off like never before and the mycelium at the top of the substrate was slightly blue and the mycelium becomes very ropey and thick completely different to any rizo myc or cottoned myc it takes on a whole new look which i find fascinating
anyway can some other people test this and let me know if i stumbled across something good or just some thoughts on what happened.
 above no h2o2
 above h2o2
 the above pic is the big jar which is even nearly finished which is from the h2o2 TIT
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Edited by Juke Adro (06/16/08 03:19 PM)
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legallyhomeless
mooch


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Re: h202 discovery [Re: Juke Adro]
#8410474 - 05/16/08 06:16 PM (16 years, 6 days ago) |
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This sounds really interesting. How did the h202 touch the mycelium in the jars? Im curious how they turned blue.
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Juke Adro
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it did not really touch them it was just a figure of speach more like i believe the humidity of the h202 breathed through the holes and foil, it was only very slight bluing, and you can see where the myc climbed the walls trying to get out of the holes very very interesting
if you can imagine a fc full of humidity with h20, but this way its the incubator and h202 humidity but only a small amount of it. i really think that it's something here the mycelium really changes from thin riz growth to very think riz growth and not cottony it also climbs the walls trying to get out and builds up near the holes to get out, but those areas are more blue and its not damaged cause it still grows fast as hell and fruits (well pinning atm)
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Edited by Juke Adro (05/16/08 06:40 PM)
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xFrockx



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Re: h202 discovery [Re: Juke Adro]
#8410865 - 05/16/08 08:27 PM (16 years, 6 days ago) |
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It would seem to make sense that the excess 02 released by the peroxide would help growth, I think this could be one of the more promising things I've seen on here in a while when it comes to simplicity.
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Sitar
Musicologist


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Re: h202 discovery [Re: xFrockx]
#8411068 - 05/16/08 09:35 PM (16 years, 6 days ago) |
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This is exactly one of the reasons that I put lots of H2O2 in the perlite of my fruiting chambers. Not only does it prevent it from getting funky, but I've always suspected that it boosts the oxygen levels in the chamber slightly. It certainly can't hurt.
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John Nada
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Re: h202 discovery [Re: Juke Adro]
#8411543 - 05/17/08 12:15 AM (16 years, 5 days ago) |
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Watch out for h202 especially if it's undiluted too much will delay or even kill mycelium.
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flavoraid
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Re: h202 discovery [Re: John Nada]
#8412129 - 05/17/08 07:47 AM (16 years, 5 days ago) |
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Quote:
paradis said: Watch out for h202 especially if it's undiluted too much will delay or even kill mycelium.
it wasn't directly applied.
-------------------- coda said: imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.
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John Nada
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Re: h202 discovery [Re: flavoraid]
#8412521 - 05/17/08 11:34 AM (16 years, 5 days ago) |
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Oh I see. Sorry, well that shouldn't hurt then
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flavoraid
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Re: h202 discovery [Re: John Nada]
#8412633 - 05/17/08 12:19 PM (16 years, 5 days ago) |
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I'll have to try this, hell I might try attaching my scuba gear to my incubator even.
IMO.. i dont think it would make that much difference because people would have realised this by now and would use larger gas exchange on there covers for casigns while incubating.
fahster has experimented with his oven bag grows and he was change a fair bit of oxygen while his substrate colonized I think multiple changes a day, didn't report anything about increased colonization rate.
-------------------- coda said: imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.
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John Nada
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Re: h202 discovery [Re: flavoraid]
#8412969 - 05/17/08 01:54 PM (16 years, 5 days ago) |
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You could also just use a fan with a filter over the intake.
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flavoraid
now with twicethe ketamine andopiates!

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Re: h202 discovery [Re: John Nada]
#8413003 - 05/17/08 02:03 PM (16 years, 5 days ago) |
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thinking of this... I actually don't think it's possible to have great increase in growth.
People have like open room incubators with virtually limitless amounts of oxygen..... that's my point :P
-------------------- coda said: imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.
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Juke Adro
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Re: h202 discovery [Re: flavoraid]
#8413372 - 05/17/08 03:24 PM (16 years, 5 days ago) |
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Quote:
flavoraid said: thinking of this... I actually don't think it's possible to have great increase in growth.
People have like open room incubators with virtually limitless amounts of oxygen..... that's my point :P
yeah but this is different, its not normal oxygen its making the incubator humid with h202 oxygen .....not normal stale dry oxygen, please read properly before you make assumptions
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Edited by Juke Adro (05/17/08 03:28 PM)
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Juke Adro
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Re: h202 discovery [Re: flavoraid]
#8413384 - 05/17/08 03:26 PM (16 years, 5 days ago) |
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Quote:
flavoraid said: I'll have to try this, hell I might try attaching my scuba gear to my incubator even.
IMO.. i dont think it would make that much difference because people would have realised this by now and would use larger gas exchange on there covers for casigns while incubating.
fahster has experimented with his oven bag grows and he was change a fair bit of oxygen while his substrate colonized I think multiple changes a day, didn't report anything about increased colonization rate.
I know what your saying but try it before you make a assumption, it really makes a big difference the stuff i use is called .....oxy plus active ingredient 50% hydrogen peroxide, im guessing the other 50% is water it does not say on the bottle what the other 50% is.
anyway you will see how the myc tries to climb out of your jars its really cool
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Grogan
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Re: h202 discovery [Re: Juke Adro]
#8413965 - 05/17/08 06:46 PM (16 years, 5 days ago) |
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What percentage concentration was it thats pretty damn important to know if we wanted to try this out!! I will give it a go for sure some day soon.
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RogerRabbit
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Re: h202 discovery [Re: Juke Adro]
#8413968 - 05/17/08 06:47 PM (16 years, 5 days ago) |
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Many growers have placed peroxide in perlite and humidifiers, but with no increased growth. To test peroxide, I once brought home an air analyzer from work and tested the O2 content of the air, then added 35% industrial grade peroxide to the humidifier(no water). There was no noticeable increase in O2 levels in the fruiting chamber.
It actually sounds more like a coincidence. However, increased oxygen during colonization would not necessarily be a good thing. We use small gas exchange holes in our lids precisely to keep the CO2 levels high during colonization. Doing so prevents the mycelium from consuming too much of the substrate during this period. Increasing the O2 content of the air is one of the main pinning triggers, and during the jar colonization stage, you don't want to introduce pinning triggers. RR
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Juke Adro
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Re: h202 discovery [Re: Grogan]
#8413973 - 05/17/08 06:49 PM (16 years, 5 days ago) |
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50% hydrogen peroxide the actual product is from growtek and called oxy plus
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Juke Adro
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Re: h202 discovery [Re: Juke Adro]
#8413985 - 05/17/08 06:53 PM (16 years, 5 days ago) |
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RR I'm talking about in the incubator....not the fc. It could very well be a coincidence but why would the mycelium change its appearance and start trying to grow out of the jars with slight bluing on the top and more blue where it tries to climb up
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Juke Adro
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Re: h202 discovery [Re: Juke Adro]
#8414017 - 05/17/08 07:02 PM (16 years, 5 days ago) |
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another observation, the substrate seems to dry out faster than normal.
I understand about the pinning but i saw no problems as yet, but in jars of grain I would not think it would but who knows? I saw no pinning until it went in the fc, when re incubating casings with a fresh layer, its what you would want is it not?
but i can only wait till someone else tries it to confirm or close the case
also RR i know you know your shit so maybe you could add some to your incubator and observe it for 3 days and get back with your findings ?
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Edited by Juke Adro (05/17/08 07:07 PM)
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flavoraid
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Re: h202 discovery [Re: Juke Adro]
#8414166 - 05/17/08 07:47 PM (16 years, 5 days ago) |
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Quote:
Juke Adro said:
Quote:
flavoraid said: thinking of this... I actually don't think it's possible to have great increase in growth.
People have like open room incubators with virtually limitless amounts of oxygen..... that's my point :P
yeah but this is different, its not normal oxygen its making the incubator humid with h202 oxygen .....not normal stale dry oxygen, please read properly before you make assumptions
uh so you'de have two parts - h202 which damages mycelium and oxygen.... all h202 does when broken down is release oxygen
-------------------- coda said: imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.
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Juke Adro
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Re: h202 discovery [Re: flavoraid]
#8414207 - 05/17/08 07:57 PM (16 years, 5 days ago) |
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its not broken fully down it's humidifying the incubator so its still hydrogen peroxide not oxygen!!!!!!!!!!!! it would be dry if it was broken down completely (I think lol) and its not damaging it cause its fine as you would notice if you read what i wrote previously.
just because the mycelium is slightly blue does not mean its fucked my guess was it grew too fast which caused that to happen and got slightly too much h202
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wisp

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Re: h202 discovery [Re: Juke Adro]
#8414397 - 05/17/08 08:37 PM (16 years, 5 days ago) |
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h2o2 still has the same amount of oxygen as h20, just an extra hydrogen atom, so i can't see it being any different to humidifying with water.
Quote:
RogerRabbit said: It actually sounds more like a coincidence. However, increased oxygen during colonization would not necessarily be a good thing. We use small gas exchange holes in our lids precisely to keep the CO2 levels high during colonization. Doing so prevents the mycelium from consuming too much of the substrate during this period. Increasing the O2 content of the air is one of the main pinning triggers, and during the jar colonization stage, you don't want to introduce pinning triggers. RR
keeping this in mind it's probable that the growth changed because of the increased oxygen levels, but it still isn't necessarily something desirable. as oxygen is a pinning trigger, if you expose your mycelium to oxygen during incubation, come fruiting it might be problematic getting it to fruit.
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Juke Adro
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Re: h202 discovery [Re: wisp]
#8414453 - 05/17/08 08:47 PM (16 years, 5 days ago) |
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yeah not sure trip, just be nice if someone else tried it so i know weather it was a coincidence or in fact quicker growth.
im skeptical too but i tried it again and same thing happened sooo i dunno 2 coincidences in a row ?
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wisp

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Re: h202 discovery [Re: Juke Adro]
#8414481 - 05/17/08 08:53 PM (16 years, 5 days ago) |
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did you notice any early pinning? maybe i'll give this a go to see what happens
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Juke Adro
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Re: h202 discovery [Re: wisp]
#8414512 - 05/17/08 09:01 PM (16 years, 5 days ago) |
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well the casing pinned very quick after i took it out but the grain did not pin and looked perfect now its in shit
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wisp

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Re: h202 discovery [Re: Juke Adro]
#8414550 - 05/17/08 09:11 PM (16 years, 5 days ago) |
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how's it in shit?
putting h202 in my incubator would probably be a good thing as i just found 2 glasses with Neurospora (pink mould) in them, a particularly pernicious contam. fuck!
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Juke Adro
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Re: h202 discovery [Re: wisp]
#8414685 - 05/17/08 09:45 PM (16 years, 5 days ago) |
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i spawned some to horse poo is what i meant lol
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Tsukishade
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Re: h202 discovery [Re: wisp]
#8415641 - 05/18/08 07:10 AM (16 years, 4 days ago) |
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Quote:
tripsis said: h2o2 still has the same amount of oxygen as h20, just an extra hydrogen atom, so i can't see it being any different to humidifying with water.
Actually, it contains another oxygen atom. H2O2 is a very strong oxidizer. It decomposes spontaneously into oxygen and water in the following exothermic reaction:
2 H2O2 → 2 H2O + O2
Also, Juke, your product is 50% peroxide solution or 50 Volume? The difference is as follows: 1% peroxide releases 3.3 volumes of oxygen during decomposition. A 10 Vol. solution is at 3% concentration, a 50 Vol. is at 15%.
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wisp

Registered: 04/13/08
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Oh shit yeah, what was I thinking? I blame being sick! Lol. Fuck that was daft.
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WeBeBad
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Re: h202 discovery [Re: Juke Adro]
#8416106 - 05/18/08 12:10 PM (16 years, 4 days ago) |
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Quote:
Juke Adro said: its not broken fully down it's humidifying the incubator so its still hydrogen peroxide not oxygen!!!!!!!!!!!! it would be dry if it was broken down completely (I think lol) and its not damaging it cause its fine as you would notice if you read what i wrote previously.
I think you've stepped out of your area of expertise to be making assumptions like these. You, yourself, already said you used a dilute solution of hydrogen peroxide. Do you know what's in a solution of hydrogen peroxide? Water and H2O2, but you should know that. Whatever observable increased humidity levels being caused are coming from WATER vapor. Hydrogen peroxide breaks down into hydrogen and oxygen gas. Please don't make silly assumptions and please don't step out of your area of expertise. It'll only serve to confuse people.
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poof
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Re: h202 discovery [Re: Juke Adro]
#8417164 - 05/18/08 04:45 PM (16 years, 4 days ago) |
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ok you found something great that works for you, whats your point in trying to convince these know-it-all losers that it works? hell, if i were you i would have kept it to myself just so others could fail harder than they already do.
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legallyhomeless
mooch


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Re: h202 discovery [Re: poof]
#8417961 - 05/18/08 08:15 PM (16 years, 4 days ago) |
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This forum is about learning not hiding information.
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wisp

Registered: 04/13/08
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agreed, it would never progress if everyone hid everything they learned and knew.
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John Nada
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Re: h202 discovery [Re: wisp]
#8419560 - 05/19/08 10:03 AM (16 years, 3 days ago) |
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Ha, this should be moved to the regular cultivation forum. I just realized this was in the Advanced forum.
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RogerRabbit
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Re: h202 discovery [Re: John Nada]
#8420250 - 05/19/08 02:24 PM (16 years, 3 days ago) |
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I'll leave it here for now, but if the thread continues to degrade with no additional data, I'll just lock it.
Personally, I think the effects of adding O2 to colonizing jars are deleterious, even if it does speed up colonization. You don't want your substrate being consumed prior to the fruiting stage, so that the nutrients in the substrate are available for the fruitbodies. That's the reason we use very small holes for gas exchange rather than simply placing a filter over the top of the jar. It's important to keep the CO2 levels high during colonization. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Juke Adro
I love peach fluff


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Quote:
Tsukishade said:
Quote:
tripsis said: h2o2 still has the same amount of oxygen as h20, just an extra hydrogen atom, so i can't see it being any different to humidifying with water.
Actually, it contains another oxygen atom. H2O2 is a very strong oxidizer. It decomposes spontaneously into oxygen and water in the following exothermic reaction:
2 H2O2 → 2 H2O + O2
Also, Juke, your product is 50% peroxide solution or 50 Volume? The difference is as follows: 1% peroxide releases 3.3 volumes of oxygen during decomposition. A 10 Vol. solution is at 3% concentration, a 50 Vol. is at 15%.
it says on the bottle 50% active ingredient hydrogen peroxide the other 50% would be just water imo but i can not be 100% cause all it says is 50% hydrogen peroxide here is the bottle
i dont dilute it just use it straight out of the bottle
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Edited by Juke Adro (05/19/08 03:30 PM)
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Juke Adro
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Re: h202 discovery [Re: WeBeBad]
#8420486 - 05/19/08 03:28 PM (16 years, 3 days ago) |
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Quote:
WeBeBad said:
Quote:
Juke Adro said: its not broken fully down it's humidifying the incubator so its still hydrogen peroxide not oxygen!!!!!!!!!!!! it would be dry if it was broken down completely (I think lol) and its not damaging it cause its fine as you would notice if you read what i wrote previously.
I think you've stepped out of your area of expertise to be making assumptions like these. You, yourself, already said you used a dilute solution of hydrogen peroxide. Do you know what's in a solution of hydrogen peroxide? Water and H2O2, but you should know that. Whatever observable increased humidity levels being caused are coming from WATER vapor. Hydrogen peroxide breaks down into hydrogen and oxygen gas. Please don't make silly assumptions and please don't step out of your area of expertise. It'll only serve to confuse people.
look mate i understand what your saying ...but i am just telling what i observed so either you can try it and see for your self or simply just ignore it, it wont confuse anyone im not a braniac but i saw a big difference and thought i would share it, weather you like it or not. I dont want negativity i just wanted to share what happened and maybe for someone to confirm or deny
and lastly i will be adding h202 in my fc till the day i die
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Juke Adro
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Re: h202 discovery [Re: Juke Adro]
#8420522 - 05/19/08 03:36 PM (16 years, 3 days ago) |
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I'm going to test it one more time, 3 all up this time i will start from ms, inoculate, spwan to poo incubate, case incubate and fruit i will document everything with times pictures the works
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Juke Adro
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Quote:
legallyhomeless said: This forum is about learning not hiding information.
ture even if its not practical at least we will know "not to add h202 to a fc" either way you find out something
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legallyhomeless
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Re: h202 discovery [Re: Juke Adro]
#8423302 - 05/20/08 07:17 AM (16 years, 2 days ago) |
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I think that if you do try this again and document it, you will need to try 1 tub w/ oxy, 1 tub w/o oxy. 1 incubator w/ oxy, 1 incubator w/o oxy. even though it will be multispore it will still be better then only having 1 thing to compare to, it will just bring more negativity
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MrCubensis
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Re: h202 discovery [Re: Juke Adro]
#8424002 - 05/20/08 12:19 PM (16 years, 2 days ago) |
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pics of anything?
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Juke Adro
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Quote:
legallyhomeless said: I think that if you do try this again and document it, you will need to try 1 tub w/ oxy, 1 tub w/o oxy. 1 incubator w/ oxy, 1 incubator w/o oxy. even though it will be multispore it will still be better then only having 1 thing to compare to, it will just bring more negativity
ok il do that and see what happens il start asap and see where it takes us
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Juke Adro
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Quote:
MrCubensis said: pics of anything?
i only have a camera on my phone 2.5 mega pixel they wont be the best but il start uploading and documenting friday as im free then
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wisp

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Re: h202 discovery [Re: Juke Adro]
#8424800 - 05/20/08 04:53 PM (16 years, 2 days ago) |
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sephiroth3535
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Re: h202 discovery [Re: wisp]
#8435894 - 05/23/08 05:18 AM (15 years, 11 months ago) |
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I just recently read this...I have some fruiting bodies going on, so I was like what the hell! I took a water bottle, filled it like 3/4 way with peroxide, and then threw some old silver dimes into the mix. WOW! Within MINUTES the coin starts bubbling like insane. Pure oxygen. Its been in there for a few hours now and is still bubbling, I don't see how this can not be good for the mushrooms! You can literally see the reaction and the oxygen going into the air, so I don't know how its even arguable that this doesn't release oxygen into your FC.
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Juke Adro
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in the incubator not the fc most of us put it in the fc but this thread is about it being in the incubator
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Digital Reality
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Re: h202 discovery [Re: Juke Adro]
#8436301 - 05/23/08 09:40 AM (15 years, 11 months ago) |
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Sorry to be off topic but god damn I hate your avatar so much. I can’t think about anything other than those fucking tits flopping around everywhere. lol that girl is so damn hot. It seems to be a love hate thing. Im like mesmerized by her but god damn I hate it cuz I can’t concentrate for more than 3 seconds on what im reading. lol
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xhooliganx
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Re: h202 discovery [Re: WeBeBad]
#8436478 - 05/23/08 10:55 AM (15 years, 11 months ago) |
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Quote:
WeBeBad said:
Quote:
Juke Adro said: its not broken fully down it's humidifying the incubator so its still hydrogen peroxide not oxygen!!!!!!!!!!!! it would be dry if it was broken down completely (I think lol) and its not damaging it cause its fine as you would notice if you read what i wrote previously.
I think you've stepped out of your area of expertise to be making assumptions like these. You, yourself, already said you used a dilute solution of hydrogen peroxide. Do you know what's in a solution of hydrogen peroxide? Water and H2O2, but you should know that. Whatever observable increased humidity levels being caused are coming from WATER vapor. Hydrogen peroxide breaks down into hydrogen and oxygen gas. Please don't make silly assumptions and please don't step out of your area of expertise. It'll only serve to confuse people.
I thought h2o2 breaks down into h2o and oxygen gas. that was my favorite part about this whole topic was that the OP is stuck on there being some kind of h2o2 humidity vapor stimulating growth in his incubator. when any kind of humidity would be h2o and there would be a slight increase in oxygen. RR already explained that increasing oxygen isn't optimal and if you wanted higher gas exchange with more oxygen you could just put a filter disc under the band with no restriction.
as for adding a scuba tank it would be more cost effective to just add a fan because a scuba tank isn't filled with oxygen it's filled with air. the same stuff that a fan would blow into a incubator.
to everyone bitching about people being negative or "brainy know it alls" if you can't handle sceptacism, logic, and science then maybe the advanced forum isn't the place to talk about experimental unproven methods.
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Juke Adro
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Re: h202 discovery [Re: xhooliganx]
#8442846 - 05/25/08 06:03 AM (15 years, 11 months ago) |
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Quote:
xhooliganx said:
Quote:
WeBeBad said:
Quote:
Juke Adro said: its not broken fully down it's humidifying the incubator so its still hydrogen peroxide not oxygen!!!!!!!!!!!! it would be dry if it was broken down completely (I think lol) and its not damaging it cause its fine as you would notice if you read what i wrote previously.
I think you've stepped out of your area of expertise to be making assumptions like these. You, yourself, already said you used a dilute solution of hydrogen peroxide. Do you know what's in a solution of hydrogen peroxide? Water and H2O2, but you should know that. Whatever observable increased humidity levels being caused are coming from WATER vapor. Hydrogen peroxide breaks down into hydrogen and oxygen gas. Please don't make silly assumptions and please don't step out of your area of expertise. It'll only serve to confuse people.
I thought h2o2 breaks down into h2o and oxygen gas. that was my favorite part about this whole topic was that the OP is stuck on there being some kind of h2o2 humidity vapor stimulating growth in his incubator. when any kind of humidity would be h2o and there would be a slight increase in oxygen. RR already explained that increasing oxygen isn't optimal and if you wanted higher gas exchange with more oxygen you could just put a filter disc under the band with no restriction.
as for adding a scuba tank it would be more cost effective to just add a fan because a scuba tank isn't filled with oxygen it's filled with air. the same stuff that a fan would blow into a incubator.
to everyone bitching about people being negative or "brainy know it alls" if you can't handle sceptacism, logic, and science then maybe the advanced forum isn't the place to talk about experimental unproven methods.
i completely see what your saying but.....if it broke down into h20 it would not burn my hands by touching the lid of the incubator......so what your hypothisis is....is just a hypothisis and just because RR said something it dont mean you have to take his advice, he is not god and il admit he knows his shit, but it dont mean it wont work in fact it does work ....it speeds up growth weather you like it or not and it does get in the jars and will burn the mycelium try it and see
it might eat up all your nutes and it might dry out your jars/casings and it might initiate fruiting ....but it makes the mycelium grow faster by at least 25% so unless you are going to do it and see for your self i dont want your hypothisis or smart arse remarks
and if i choose to put it in advanced mycology thats where it will be.
Adding h2o2 in the incubator makes mycelium grow faster weather its good for it or not thats my fucking point now lets see you come up with anything that works, all i am trying to do is show what i found not make a argument
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Seuss
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Re: h202 discovery [Re: Juke Adro]
#8442892 - 05/25/08 06:35 AM (15 years, 11 months ago) |
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> and if i choose to put it in advanced mycology thats where it will be.
And if I choose to move it to the dump, that is where it will remain. Enough with the attitude. That goes for everybody.
> but it makes the mycelium grow faster by at least 25%
Have you performed a controlled experiment? Has it been repeated by others? If not, then you are guessing. (A controlled experiment would compare growth with and without the h2o2, holding everything else equal, in several different environments, to ensure that it is the h2o2 and not something else that is causing the increase in growth.)
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Juke Adro
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Re: h202 discovery [Re: Seuss]
#8442899 - 05/25/08 06:39 AM (15 years, 11 months ago) |
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Yeah im doing at as we speak.
I have asked other to do it also, I think tripsis said he is going to do it in pm
Sorry about my attitude if I was rude I'm sorry, I just cant handle people picking without trying it, my bad.
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RogerRabbit
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Re: h202 discovery [Re: Juke Adro]
#8444072 - 05/25/08 04:37 PM (15 years, 11 months ago) |
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If you think this is bad, try going to a scientific research site. They're rabid, but if it passes muster with them, you then have something to take to the bank.
As Seuss said, can the attitudes. I almost dumped this thread last week, but decided to let it run its course.
Perhaps the O2 is helping to make up for the lost rate of growth due to having your jars in a silly incubator instead of where they can have air circulating around them like you're supposed to do? If you have jars stuck down in a tub, there's no doubt they're sitting in stale air and O2 would help offset that somewhat. However, IF that's the case, the same increase in growth(or loss of the decrease) would be achieved by placing the jars on an open shelf. Remember, many controlled experiments have shown virtually no increase in rate of growth from 75F to 82F, and above 82F, growth begins to fall off. If you have 'normal room temperature' which is mid 70's you don't need that box anyway. RR
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ozgrove
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Hey people, Wsup ? I don`t know why peroxide on Incubating chamber would be a detriment. This is some GOOD SHIT!! Just clik this!Then clik on FAQ on Peroxide. Read this , and make up your mind, whether you like H2O2 or not!!
Peroxide
Later, OZGROVE
Edited by ozgrove (05/25/08 11:26 PM)
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Juke Adro
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Quote:
RogerRabbit said: If you think this is bad, try going to a scientific research site. They're rabid, but if it passes muster with them, you then have something to take to the bank.
As Seuss said, can the attitudes. I almost dumped this thread last week, but decided to let it run its course.
Perhaps the O2 is helping to make up for the lost rate of growth due to having your jars in a silly incubator instead of where they can have air circulating around them like you're supposed to do? If you have jars stuck down in a tub, there's no doubt they're sitting in stale air and O2 would help offset that somewhat. However, IF that's the case, the same increase in growth(or loss of the decrease) would be achieved by placing the jars on an open shelf. Remember, many controlled experiments have shown virtually no increase in rate of growth from 75F to 82F, and above 82F, growth begins to fall off. If you have 'normal room temperature' which is mid 70's you don't need that box anyway. RR
yeah you may be right, thats why I'm testing it so I should be able to give some really good answers in 10 days.
2 jars in a open room on a shelf, 2 jars in a tub in tub with 50mls h2o2 held at 75f and 2 and another tub in tub with no h2o2 held at 75f
God, you guys are really set in your ways yeah.
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wisp

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Re: h202 discovery [Re: Juke Adro]
#8446090 - 05/26/08 07:10 AM (15 years, 11 months ago) |
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It the scientific approach. It needs to be rigourous. But it'll be all the better if you get positive results.
Edited by wisp (05/26/08 07:15 AM)
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JaComet
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Re: h202 discovery [Re: ozgrove]
#8446102 - 05/26/08 07:26 AM (15 years, 11 months ago) |
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H2O2 based oxygen products intended for agricultural use are stabilized with additives in order to control natural reactivity of the product, which increases with temperature.
H2O2 does give off reactive vapor which, in higher concentrations, will cause notable sanitizing / bleaching.
H2O2 can be carried by water vapor at any concentration level.
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Juke Adro
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Re: h202 discovery [Re: wisp]
#8446141 - 05/26/08 07:57 AM (15 years, 11 months ago) |
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Quote:
tripsis said: It the scientific approach. It needs to be rigourous. But it'll be all the better if you get positive results.
I better get positive results otherwise these guys in here will say ....I told ya so, and il look like a fool but either way I'm happy to provide a result weather it is good or bad.
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Juke Adro
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Re: h202 discovery [Re: JaComet]
#8446150 - 05/26/08 08:05 AM (15 years, 11 months ago) |
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Quote:
JaComet said: H2O2 based oxygen products intended for agricultural use are stabilized with additives in order to control natural reactivity of the product, which increases with temperature.
H2O2 does give off reactive vapor which, in higher concentrations, will cause notable sanitizing / bleaching.
H2O2 can be carried by water vapor at any concentration level.
Thank you, that explains why my knuckles turned white and burned when they touched the inside of the lid.
So now it would also explain why the mycelium turns blue on the surface of the substrate
so we are getting somewhere.
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JaComet
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Re: h202 discovery [Re: Juke Adro]
#8454063 - 05/28/08 06:11 AM (15 years, 11 months ago) |
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You're welcome JA.
Be certain to treat this stuff with due respect and caution. 50% is a very high concentration. I note the current Oxy-Plus product is listed at 35%, which is the concentration used in aerobic sterilization of food packaging.
Keep liquid and vapors away from eyes and mucus membranes.
It degrades most plastics.
If opting to use Food Grade, store in freezer.
Good luck with your trials.
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Juke Adro
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Re: h202 discovery [Re: JaComet]
#8475586 - 06/02/08 04:29 PM (15 years, 11 months ago) |
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ok I know It has taken some time but I'm too busy with work,
ok so normal drinking glasses were observed to have had the boosted growth so its drinking glasses I will use and brown rice was the substrate so brown rice will be used the only thing im not 100 percent sure of was how much h2o2 to the mls so i will use 50ml of 50% h202
5 cups were inoculated today at 6am with multi spore syringes all of the same strain 2cc were used per cup 10cc all up
1 cup sit's on the shelf in a room at temps between 14C and 20C
2 cups sit in a TIT at a constant 23C with 50ml h202 chamber is 80ltrs
2 cups will sit in a TIT at a constant 23C with no h2o2 chamber is 80ltrs
I'm so confident it works that I put one large jar not sure of the size and a larger cup in the h2o2 TIT cause Im sure they will be fully colonised before the other non h2o2 ones
anyway one more note I might have to add more h2o2 in the TIT if it runs out but i will let you know if i do and how much .....this is my first test ever besides school so if you want to help please tell me how to make it better..

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wisp

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Re: h202 discovery [Re: Juke Adro]
#8476143 - 06/02/08 06:35 PM (15 years, 11 months ago) |
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Interesting man. I can't help with the experiment anymore, I deconstructed my TiT. But this is certainly something to keep in mind.
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RogerRabbit
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Re: h202 discovery [Re: wisp]
#8477521 - 06/02/08 10:54 PM (15 years, 11 months ago) |
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Quote:
1 cup sit's on the shelf in a room at temps between 14C and 20C
Note that when we say normal room temperature, it's a well defined range. 20C to 25C or 68F to 77F. Google it.
Your jars will still colonize at 20C, but will be slower. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Juke Adro
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well i fucked that up now,
give me till tomorrow morning and i will work out something to keep the temp at that range.
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wisp

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Re: h202 discovery [Re: Juke Adro]
#8477716 - 06/02/08 11:48 PM (15 years, 11 months ago) |
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The joy of living in Melbourne huh? 
Do what I do. Get one of those little kambrook heaters with a thermostat. Set it on it's lowest setting and turn it on. Whenever in reaches that temp it switches off, and turns back on when it drops below it. Keeps the room at a pretty steady temp ~ 25C. It should be a small room though.
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Juke Adro
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Re: h202 discovery [Re: wisp]
#8480530 - 06/03/08 06:14 PM (15 years, 11 months ago) |
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its done i just put a heat mat from a reptile cage and its timer in the cupboard i set it and seems to be working great its a big heat mat so should be fine.
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Juke Adro
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Re: h202 discovery [Re: Juke Adro]
#8484317 - 06/04/08 05:07 PM (15 years, 11 months ago) |
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ok they all have growth, I will take pics and post them tonight
A little observation noted, was the substrate in the h2o2 jars seemed to be a little more dry than usual, which is a bad thing but the test continues.
Pics coming tonight/tomorrow
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xhooliganx
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Re: h202 discovery [Re: Juke Adro]
#8496099 - 06/07/08 12:10 PM (15 years, 11 months ago) |
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Quote:
Juke Adro said: its done i just put a heat mat from a reptile cage and its timer in the cupboard i set it and seems to be working great its a big heat mat so should be fine.
ok so if the test group that is supposed to be in an open room at roomtemp is in a closed cupboard with a heat mat isn't that more like having it in a wooden incubator?
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Juke Adro
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Re: h202 discovery [Re: xhooliganx]
#8496442 - 06/07/08 02:28 PM (15 years, 11 months ago) |
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have you seen my cupboards its a walk in wardrobe so its huge and i walk in and out of it everyday its on the top shelf and gets heaps of air, also the wardrobe is prolly as big as a small room so its fine and that one is not going the best either as i suspected
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xhooliganx
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Re: h202 discovery [Re: Juke Adro]
#8497098 - 06/07/08 06:58 PM (15 years, 11 months ago) |
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Quote:
Juke Adro said: have you seen my cupboards its a walk in wardrobe so its huge and i walk in and out of it everyday its on the top shelf and gets heaps of air, also the wardrobe is prolly as big as a small room so its fine and that one is not going the best either as i suspected
oh I thought you meant a small cupboard because I didn't think a reptile heating matt woul bring the air temp in a small room up 5 C.
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Juke Adro
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Re: h202 discovery [Re: xhooliganx]
#8497114 - 06/07/08 07:06 PM (15 years, 11 months ago) |
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the heat mat is coiled around the glass not touching it ...it only gets to 22C so I didn't even need the thermostat and its got shit loads of air around it
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Juke Adro
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Re: h202 discovery [Re: Juke Adro]
#8501355 - 06/08/08 08:01 PM (15 years, 11 months ago) |
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Ok the results are in ......here are some shitty pics but you can still see whats going on
The jars in the TIT with h2o2 are almost 100% colonised


The jars in the TIT with no h2o2 only have growth at the inoculation site 2 inches by 2 inches  
the jar by him self is about 70% colonised
so i find that a TIT is not good for incubation but if you add h2o2 it can work better than incubation in a open space ...i will keep on using my TIT with h2o2 from now on
if thats not enough proof that its working do it your self and then talk to me lol
but please try this I'm not good at these experiments and my pics are crap so if you want to try this please do and put a link in this thread so we can all watch it. This may have been luck and may have been a pure fluke but I doubt it I will try get a better camera tomorrow and take some better pics
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wisp

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Re: h202 discovery [Re: Juke Adro]
#8501397 - 06/08/08 08:11 PM (15 years, 11 months ago) |
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Nice job man.
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Juke Adro
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Re: h202 discovery [Re: wisp]
#8501407 - 06/08/08 08:14 PM (15 years, 11 months ago) |
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Thanks mate I'm ashamed of my shitty pics tho I will grab a good camera and post better pics tomorrow so all can see it perfectly
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FirstAvailable
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Re: h202 discovery [Re: Juke Adro]
#8502340 - 06/09/08 02:07 AM (15 years, 11 months ago) |
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This is interesting, however, I don't think the product you are using is 50% H2O2. It might be, but I don't think so. H2O2 in high concentrations is considered a hazardous material. The highest concentration that I am aware of that is easy to obtain commercially without special permits is 35%. Most likely, it is 50% H2O2 solution, with the remaining 50% additives for growth.
Have you tried doing this experiment using just regular H2O2, like the kind you would get at a drug store? This would be a good idea in order to rule out the chance that the other "mystery stuff" in your product is the cause of the added growth rate.
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makaveli8x8
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so you have glasses with foil ontop currect and nothing else? Well i kind of have a hypothesis then as to why this may be helping growth, its keeping everything alot cleaner
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RogerRabbit
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We specifically use small gas exchange holes in our jar lids to keep CO2 at a high level during colonization. You don't want lots of O2. The reason is to prevent the mycelium from consuming too much of the substrate prior to fruiting.
Increased O2 is a major pinning trigger. If you colonzize the jars in a high O2 environment, not only does the mycelium consume more of the substrate that you want to save for fruiting, you also remove a major pinning trigger when you move the substrates to your fruiting environment. It will be interesting to see how these fruit, how many flushes, etc. RR
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Juke Adro
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Im really sorry about my crap pics here are some better ones
 above no h2o2
 above h2o2
 the above pic is the big jar which is even nearly finished which is from the h2o2 TIT
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Juke Adro
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Quote:
FirstAvailable said: This is interesting, however, I don't think the product you are using is 50% H2O2. It might be, but I don't think so. H2O2 in high concentrations is considered a hazardous material. The highest concentration that I am aware of that is easy to obtain commercially without special permits is 35%. Most likely, it is 50% H2O2 solution, with the remaining 50% additives for growth.
Have you tried doing this experiment using just regular H2O2, like the kind you would get at a drug store? This would be a good idea in order to rule out the chance that the other "mystery stuff" in your product is the cause of the added growth rate.
Yes it is 50% h2o2 no additives and yes it is very dangerous. I have had multiple burns with this product and had a few other issues with it .....I have a good friend at the hydro shop where I buy my equipment
No I have not used regular h2o2 I only use 50%
I'm also not willing to do anymore testing of it i will just spawn and show my pics so you all can see it does not affect growth
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Juke Adro
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Quote:
RogerRabbit said: We specifically use small gas exchange holes in our jar lids to keep CO2 at a high level during colonization. You don't want lots of O2. The reason is to prevent the mycelium from consuming too much of the substrate prior to fruiting.
Increased O2 is a major pinning trigger. If you colonzize the jars in a high O2 environment, not only does the mycelium consume more of the substrate that you want to save for fruiting, you also remove a major pinning trigger when you move the substrates to your fruiting environment. It will be interesting to see how these fruit, how many flushes, etc. RR
I will record everything and take pictures on the way from spawning which will be done asap, i just have to pasteurise my horse poo, I will only use h2o2 test jars to spawn, that poo which I spawn too will also go in the h2o2 TIT
so please keep this thread open till my last picture of my last flush is finished and then hopefully someone else can give it a shot, and give us there feed back
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Edited by Juke Adro (06/09/08 05:03 PM)
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wisp

Registered: 04/13/08
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Re: h202 discovery [Re: Juke Adro]
#8504454 - 06/09/08 05:39 PM (15 years, 11 months ago) |
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Nice new pics man. God strong growth. It's been very interesting following this. It's hard to argue with results. Strange coloured myc there though.
By the way, where do you get 50% H2O2 from?
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Juke Adro
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Re: h202 discovery [Re: wisp]
#8504499 - 06/09/08 05:51 PM (15 years, 11 months ago) |
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I get 50% from a mate he owns a shop down here...I asked him if it was really 50% a while back when someone else doubted that i had 50%, my mate told me to tell them to get stuffed lol and assured me that it is. also any test is welcome on it .....
and trip if you will do this experiment I will send you one brand new bottle of oxyplus h2o2 50%.
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wisp

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Re: h202 discovery [Re: Juke Adro]
#8504514 - 06/09/08 05:54 PM (15 years, 11 months ago) |
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lol maybe i'll have to reconstruct my TiT then aye!
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Juke Adro
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Re: h202 discovery [Re: wisp]
#8504726 - 06/09/08 06:55 PM (15 years, 11 months ago) |
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7 days from spores to colonised, I am so fucking amazed. It's gotta be some record.
anyway I have to run
Tripsis thanks for accepting the offer in PM I will send you one bottle in the next few days look out for it in the mail.
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RogerRabbit
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Re: h202 discovery [Re: Juke Adro]
#8507867 - 06/10/08 03:56 PM (15 years, 11 months ago) |
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Quote:
and trip if you will do this experiment I will send you one brand new bottle of oxyplus h2o2 50%
Check the regulations in your country. If it's considered hazardous(which it is) you can't send it through the mail. Perhaps a shipping company?
Don't worry about the thread getting closed-it ain't gonna happen. We just wanted everyone to show professional courtesy and respect. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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wisp

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Seuss
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> We specifically use small gas exchange holes in our jar lids to keep CO2 at a high level during colonization.
You are correct, of course. However, you are assuming that whatever is going on is releasing free O2. What if the oxygen is oxidizing something... *shrug*
Regardless, it will be interesting to see how fruiting compares.
-------------------- Just another spore in the wind.
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Seuss
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> I get 50% from a mate he owns a shop down here...
Please be very careful with that. If you really have 50% h2o2, it is very hazardous. (You can get 30%, 50%, and 70% commercially, and 90% to 100% if you really need it- namely for space/rocket applications.) Again, this stuff is nasty at 30% and very nasty at 50%. Respect it like you would a stick of dynamite. Seriously.
Edit: Also, at 30% and greater, this stuff is used in a lot of common improvised 'splozivs' (so to speak). Trying to purchase it (the commercial concentrated type) is likely to draw some unwanted attention from the uglier side of the 'shepard'.
-------------------- Just another spore in the wind.
Edited by Seuss (06/11/08 03:17 PM)
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Juke Adro
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Re: h202 discovery [Re: Seuss]
#8512077 - 06/11/08 05:01 PM (15 years, 11 months ago) |
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I do treat it very well, I have had some nasty burns already and breathed some in a while back and had no sense of smell for one week, I am taking no more chances and use a painters mask as the fumes are very strong, I wear protective gloves and glasses also 
I will get it shipped to Tripsis all will be fine, It's going to be good seeing someone else test it
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wisp

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Re: h202 discovery [Re: Juke Adro]
#8512400 - 06/11/08 06:21 PM (15 years, 11 months ago) |
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Looks like I'll want to practise caution with it then. Have to love dangerous chemicals.
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QuantumReality
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Re: h202 discovery [Re: wisp]
#8514099 - 06/12/08 04:56 AM (15 years, 11 months ago) |
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Yea carefull not to fuck yourself up with fumes, it can nock you out quick with the right shit
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wisp

Registered: 04/13/08
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Maybe do it outside? Not in an enclosed space seems like a good idea, although it will have to become one at some point. I'm not forking out for a gas/chemical mask...
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QuantumReality
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Re: h202 discovery [Re: wisp]
#8514189 - 06/12/08 05:51 AM (15 years, 11 months ago) |
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yea just a dust mask will do it. Outside would be ok
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wisp

Registered: 04/13/08
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A dust mask will do nothing to protect from chemical fumes. They do very little at all really, only protect from dust.
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makaveli8x8
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Re: h202 discovery [Re: wisp]
#8514202 - 06/12/08 06:00 AM (15 years, 11 months ago) |
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they don't form a perfect seal so they are completely useless
--------------------
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wisp

Registered: 04/13/08
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Spot on. Some protection from dust (not complete), useless for everything else.
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legallyhomeless
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Quote:
Dizzwizzle said: yea just a dust mask will do it.
wearing a dust mask and cleaning wiht 90% ISO is nasty.
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Juke Adro
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I have been busy with work and family but.....I did manage to get some shit done, I pasteurised some horse poo yesterday for 3 hours and let it cool over night, I will spawn to the horse poop tonight and put some pictures up.
thanks for watching
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Juke Adro
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Re: h202 discovery [Re: Juke Adro]
#8530372 - 06/16/08 03:27 PM (15 years, 10 months ago) |
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Ok on 13-6-08 I spawned my jars to horse poo here are a few shitty pics to show you   
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johnm214



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Re: h202 discovery [Re: Seuss]
#8531273 - 06/16/08 07:55 PM (15 years, 10 months ago) |
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Quote:
Seuss said: > I get 50% from a mate he owns a shop down here...
Please be very careful with that. If you really have 50% h2o2, it is very hazardous. (You can get 30%, 50%, and 70% commercially, and 90% to 100% if you really need it- namely for space/rocket applications.) Again, this stuff is nasty at 30% and very nasty at 50%. Respect it like you would a stick of dynamite. Seriously.
Edit: Also, at 30% and greater, this stuff is used in a lot of common improvised 'splozivs' (so to speak). Trying to purchase it (the commercial concentrated type) is likely to draw some unwanted attention from the uglier side of the 'shepard'.
Yeah really.
In chemistry classes and research, the most I've ever used or even seen around is 30%, cuz it gets dangerous after that and really isn't needed for any application I'm aware of. The 30% stuff is pretty active.
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Juke Adro
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Re: h202 discovery [Re: johnm214]
#8531335 - 06/16/08 08:07 PM (15 years, 10 months ago) |
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you must not have friends that own shops like mine then and if you think 30% is pretty active, you should have a play with 50%
It's not that hard to come by here mate, its mainly sold to farmers for commercial hydroponics
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legallyhomeless
mooch


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Re: h202 discovery [Re: Juke Adro]
#8531751 - 06/16/08 10:03 PM (15 years, 10 months ago) |
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commercial hydro
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wisp

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QuantumReality
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Quote:
legallyhomeless said:
Quote:
Dizzwizzle said: yea just a dust mask will do it.
wearing a dust mask and cleaning wiht 90% ISO is nasty.
True, Better than no mask at all... just dont stick ya head right over where ya cleaning and youll be k
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blackout


Registered: 07/16/00
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Quote:
RogerRabbit said:Personally, I think the effects of adding O2 to colonizing jars are deleterious, even if it does speed up colonization. You don't want your substrate being consumed prior to the fruiting stage, so that the nutrients in the substrate are available for the fruitbodies.
Some may value the alleged speeding up more than the decrease in yield. In another thread you mentioned people not being concerned with BE, which is true, grain is cheap.
Homebrewers will aerate their wort prior to pitching yeast, the additional oxygen promotes a fast yeast growth, this ensures a vigorous brew to take off quickly. This speeds up the brew time, and decreases the potential for contams to take hold. Oxygen is then restricted since you do not want the sugars used up making new yeast rather than alcohol.
This could work in a similar way, speed up colonisation so contams have less chance to take hold. You sacrifice some of your substrate to get this.
If something reduced the maturation time of sclerotia to 50%, but lowered the yield to 50% then many might do it.
BTW My grow area is usually only 16C, so I do incubate http://en.wikipedia.org/wiki/Room_temperature Most here tend to be growing in bedrooms, where 18C (64F) is the "recommended room temp". If you want air circulating in a colder room then you could put the jars on electronic devices in standby mode, like dvd players
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MushHunter08
Mycological Pupil



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Re: h202 discovery [Re: blackout]
#8535125 - 06/17/08 08:24 PM (15 years, 10 months ago) |
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I'm curious to see the results of further testing of this possible variable. I would try it, but not until I see more results for myself.
-------------------- "The road to excess leads to the palace of wisdom...for we never know what is enough until we know what is more than enough." -William Blake- The most simple method of growing mushrooms: www.mushroomvideos.com MultiSync's lazy bastard print/syringe guide
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Juke Adro
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I'm just hoping that the juice is worth the squeeze.
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wisp

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Re: h202 discovery [Re: Juke Adro]
#8535409 - 06/17/08 10:05 PM (15 years, 10 months ago) |
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Here's an interesting bit of information. I have some petri dishes with Ps. cubensis Orissa strain culture on them. Half are plain MEA agar, the other half H2O2 MEA agar. The ones with the H2O2 are all doing far better than the ones without. This in my experience is quite unusual, the H2O2 usually slows the growth of mycelium markedly but I'm having very different results with this particular strain.
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Juke Adro
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Re: h202 discovery [Re: wisp]
#8535423 - 06/17/08 10:09 PM (15 years, 10 months ago) |
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Do you mean h2o2 in your actual MEA or in the TIT with the MEA?
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wisp

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Re: h202 discovery [Re: Juke Adro]
#8535453 - 06/17/08 10:19 PM (15 years, 10 months ago) |
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H2O2 in the actual MEA. Different to what you're talking about, I know, but still relevant.
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Juke Adro
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Re: h202 discovery [Re: wisp]
#8535458 - 06/17/08 10:21 PM (15 years, 10 months ago) |
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cool I wonder how that works, either way it is good
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wisp

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Re: h202 discovery [Re: Juke Adro]
#8535465 - 06/17/08 10:23 PM (15 years, 10 months ago) |
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Yeah I'm not too sure really, it's quite strange. Usually I've had the polar opposite effects as mycelium really doesn't like H2O2.
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LesChanga
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Re: h202 discovery [Re: blackout]
#8537049 - 06/18/08 12:42 PM (15 years, 10 months ago) |
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Quote:
blackout said If something reduced the maturation time of sclerotia to 50%, but lowered the yield to 50% then many might do it.
this is already true... if you reduce the incubation time, you will harvest less sclerotia...
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blackout


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Re: h202 discovery [Re: wisp]
#8537304 - 06/18/08 02:32 PM (15 years, 10 months ago) |
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Quote:
tripsis said:the H2O2 usually slows the growth of mycelium markedly but I'm having very different results with this particular strain.
If you added it to hot agar it may have degraded quickly turning to oxygen and not having much of a disinfectant/slowing effect.
Edited by blackout (06/18/08 02:39 PM)
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blackout


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Re: h202 discovery [Re: LesChanga]
#8537316 - 06/18/08 02:38 PM (15 years, 10 months ago) |
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Quote:
LesChanga said:
Quote:
blackout said If something reduced the maturation time of sclerotia to 50%, but lowered the yield to 50% then many might do it.
this is already true... if you reduce the incubation time, you will harvest less sclerotia...
Yep, you got me there! Most say mature sclerotia is stronger though, maybe sclerotia is not the best example. But do you see my point? say your cube grow of 1 kilo of dry grain gave a fresh yield of 1 kilo over 6 weeks. If you could get 1/2 kilo in 3 weeks many might prefer it. People could be in a rush, going away on holidays etc. Many will toss grows that could get another small flush from, not being worth their while.
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Kagenical
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Just figured I'd chime in here... I had 3 jars that were just *not* colonizing fully... They smelled fine, and showed no signs of bacterial contamination... They wouldn't colonize the bottom even after I flipped them upside down.
I added 100ml of H2o2 into the bottom of a small Tupperware container (about 1cm up the jars), and put them in it, and closed the lid...
Came back 3 days later to find that one had fully colonized... And two hadn't moved at all.
Unforuntately, one of the ones that hadn't fully colonized yet pinned invitro (I'd assume from the burst of oxygen and lower temperature due to evaporation of the H2o2 that was covering the bottom of the glass jars.
Not sure what this means, and I didn't bother to read the entire thread, but I figured I'd relay my results.
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abitavenger
3rd gear vtak



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Re: h202 discovery [Re: Kagenical]
#8538483 - 06/18/08 08:36 PM (15 years, 10 months ago) |
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interesting thread
this is next on my list of need to trys
-------------------- Karma.
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wisp

Registered: 04/13/08
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Re: h202 discovery [Re: blackout]
#8538921 - 06/18/08 10:48 PM (15 years, 10 months ago) |
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Quote:
blackout said:
Quote:
tripsis said:the H2O2 usually slows the growth of mycelium markedly but I'm having very different results with this particular strain.
If you added it to hot agar it may have degraded quickly turning to oxygen and not having much of a disinfectant/slowing effect.
Nope, that certainly didn't happen. I added it when it was cool enough to handle. I also knocked up a few other dishes with another species and had the polar opposite effect. This culture hates the H2O2 but is growing fine on the agar without, so it definitely has not degraded.
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Juke Adro
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Re: h202 discovery [Re: Kagenical]
#8539123 - 06/18/08 11:47 PM (15 years, 10 months ago) |
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Quote:
Kagenical said: Just figured I'd chime in here... I had 3 jars that were just *not* colonizing fully... They smelled fine, and showed no signs of bacterial contamination... They wouldn't colonize the bottom even after I flipped them upside down.
I added 100ml of H2o2 into the bottom of a small Tupperware container (about 1cm up the jars), and put them in it, and closed the lid...
Came back 3 days later to find that one had fully colonized... And two hadn't moved at all.
Unforuntately, one of the ones that hadn't fully colonized yet pinned invitro (I'd assume from the burst of oxygen and lower temperature due to evaporation of the H2o2 that was covering the bottom of the glass jars.
Not sure what this means, and I didn't bother to read the entire thread, but I figured I'd relay my results.
Of course it would have pinned lol but my silly theory is that if you do it from the start the myc gets use to it or whatnot.
what percentage was your h2o2?
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MushHunter08
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Re: h202 discovery [Re: Juke Adro]
#8540513 - 06/19/08 11:39 AM (15 years, 10 months ago) |
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Tripsis exactly what amount of H2O2 did you add the the petri dish with the Orissa?
-------------------- "The road to excess leads to the palace of wisdom...for we never know what is enough until we know what is more than enough." -William Blake- The most simple method of growing mushrooms: www.mushroomvideos.com MultiSync's lazy bastard print/syringe guide
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wisp

Registered: 04/13/08
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1ml of 3% H2O2 to 150ml of agar. It sounds strange I know, but the Orissa culture seems to love it yet the other species I have going wouldn't touch it and I had to transfer them last night.
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flavoraid
now with twicethe ketamine andopiates!

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Re: h202 discovery [Re: wisp]
#8547242 - 06/21/08 11:28 AM (15 years, 10 months ago) |
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prehaps a better idea is your orissa isolation is just more aggresive.
h2o2 attacks living cells.
-------------------- coda said: imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.
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wisp

Registered: 04/13/08
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Re: h202 discovery [Re: flavoraid]
#8547255 - 06/21/08 11:33 AM (15 years, 10 months ago) |
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It is more aggressive undoubtedly. But still, on the plates with H2O2 the Orissa did better than on the plates without. This is by no means a controlled experiment, just an observation.
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Juke Adro
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Re: h202 discovery [Re: wisp]
#8553154 - 06/23/08 04:45 AM (15 years, 10 months ago) |
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I will take the foil off tonight and take a pic of how the spawned poo looks i will post some pics in a day or 2....lets see if its ready or not to be fruited.
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MushHunter08
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Re: h202 discovery [Re: Juke Adro]
#8558595 - 06/24/08 02:25 PM (15 years, 10 months ago) |
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Come on with those pics man. This is a very interesting thread to me.
-------------------- "The road to excess leads to the palace of wisdom...for we never know what is enough until we know what is more than enough." -William Blake- The most simple method of growing mushrooms: www.mushroomvideos.com MultiSync's lazy bastard print/syringe guide
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BlimeyGrimey
Collector of Spores




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Re: h202 discovery [Re: blackout]
#8558608 - 06/24/08 02:29 PM (15 years, 10 months ago) |
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Quote:
blackout said: People could be in a rush, going away on holidays etc. Many will toss grows that could get another small flush from, not being worth their while.
Its always best to bury spent cakes in the yard or garden or even throw them in a compost pile. Throwing them in the trash is a complete waste.
-------------------- Message me for free microscopy services on Psilocybe, Panaeolus, and Gymnopilus species. Looking for wild Panaeolus cinctulus and Panaeolus olivaceus prints.
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Juke Adro
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Ok sorry about how it took so long but the news is that the spawn stalled and the long awaited answer is that..... the first two of my test must have been luck it seems that the h2o2 grain is a no go it may colonise fast as hell but it just wont continue to grow the poo i used is prime and it just wont take....
on the other hand the ones that were not exposed to a stupid amount of h2o2 have been spawned to the same shit and already fully colonised it and have been cased and its popping through and is 2 days off being fruited.
so there you go
I will post pics tomorrow same time same place I promice, I'm at work atm sorry guys
thanks for watching
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wisp

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Re: h202 discovery [Re: Juke Adro]
#8565662 - 06/26/08 07:12 AM (15 years, 10 months ago) |
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Ah well, can't say you didn't try. At least you gave it a proper go and wasn't daunted by other peoples doubts.
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Juke Adro
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Re: h202 discovery [Re: wisp]
#8565677 - 06/26/08 07:21 AM (15 years, 10 months ago) |
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I cant believe it lol it was so promising I suppose the myc had enough it will still fruit one day but just not anytime soon
should listened to RR 120 posts back ha ha ha oh well
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wisp

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Re: h202 discovery [Re: Juke Adro]
#8565681 - 06/26/08 07:27 AM (15 years, 10 months ago) |
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Well it wouldn't be science with out testing. No point in believing everything you are told. Experience is the best way to learn.
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makaveli8x8
Stranger


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Re: h202 discovery [Re: Juke Adro]
#8565702 - 06/26/08 07:38 AM (15 years, 10 months ago) |
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hmm so your saying it grew fine until you spawned it...your spawning containers where are they?
i ask because, the jars that were around the h2o2, if they were indeed growing quicker due to the h2o2, purhaps they became selective to it. in other words they depended on it, and were producing certain emzemes to bread it down to speed up there growth, and when you removed that growth facter they were stuck without it.
so if your poo trays arn't near the h2o2 move them back? or if thats where they already are just ignore my ramblings
--------------------
  We were sent to hell for eternity Ø h® We play on earth to pass the time Over-population the root of all Evil-brings the Elites Closer to the gates.
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wisp

Registered: 04/13/08
Posts: 5,304
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Junkie fungi huh?
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Juke Adro
I love peach fluff


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Quote:
makaveli8x8 said: hmm so your saying it grew fine until you spawned it...your spawning containers where are they?
i ask because, the jars that were around the h2o2, if they were indeed growing quicker due to the h2o2, purhaps they became selective to it. in other words they depended on it, and were producing certain emzemes to bread it down to speed up there growth, and when you removed that growth facter they were stuck without it.
so if your poo trays arn't near the h2o2 move them back? or if thats where they already are just ignore my ramblings
yeah it was with h2o2 the whole time, so i think it just changed to fruit mode????
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blackout


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Re: h202 discovery [Re: wisp]
#8566704 - 06/26/08 01:09 PM (15 years, 10 months ago) |
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Quote:
tripsis said: No point in believing everything you are told. Experience is the best way to learn.
Exactly, a lot of stuff people do in all sorts of hobbies and activities would have been laughed at, or told not to bother with at one stage.
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Juke Adro
I love peach fluff


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Re: h202 discovery [Re: blackout]
#8570024 - 06/27/08 06:26 AM (15 years, 10 months ago) |
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First pic is the h2o2, second is non h2o2...the second one fully colonised the poo and is now cased in 50/50 and gypsum mix and is popping through already.
the h2o2 is still colonising but very very slow, I will fruit it when ever it decides to finish colonising the horse poo, I'm not going to case it or we will be here till Christmas lol i will update every few days

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MushHunter08
Mycological Pupil



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Re: h202 discovery [Re: Juke Adro]
#8584251 - 07/01/08 02:35 PM (15 years, 10 months ago) |
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Hey Juke Adro, when you don't get what you want. Experience is what you get. I think it was fantastic that you were so hard headed and continued the experiment though. Even though RR was right. Nice job anyway brother!
-------------------- "The road to excess leads to the palace of wisdom...for we never know what is enough until we know what is more than enough." -William Blake- The most simple method of growing mushrooms: www.mushroomvideos.com MultiSync's lazy bastard print/syringe guide
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Seuss
Error: divide byzero



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Re: h202 discovery [Re: Juke Adro]
#8586832 - 07/02/08 06:00 AM (15 years, 10 months ago) |
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> and the long awaited answer is that..... the first two of my test must have been luck
I don't buy it (luck that is). In science, failures are just as important (and often more important) than successes. Form a new hypothesis to test to figure out why your first grow was so much better. Something has to be different... we are fairly certain it isn't the peroxide, based upon you testing, so what else could it be?
-------------------- Just another spore in the wind.
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flavoraid
now with twicethe ketamine andopiates!

Registered: 12/05/07
Posts: 1,678
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Quote:
MushHunter08 said: Hey Juke Adro, when you don't get what you want. Experience is what you get. I think it was fantastic that you were so hard headed and continued the experiment though. Even though RR was right. Nice job anyway brother!
agreed.
Experimenting is needed for mycology to progress.
-------------------- coda said: imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.
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Juke Adro
I love peach fluff


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Re: h202 discovery [Re: flavoraid]
#8592780 - 07/03/08 07:19 PM (15 years, 10 months ago) |
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Well The first time it was a accident so i was not sure exactly how much peroxide was left in the tub, so I estimated for the second, which was the exact same amount this as in this test, I am not experienced enough to understand what happened or how this time it failed well not failed but is just so slow???
it seems that from inoculation to spawn time its super fast for some reason and without peroxide its normal
update on the h2o2 tub is fully colonised  it is now cased and put in fruiting conditions but I did not take a pic of the casing
Il pop pics up when it fruits and when the non h2o2 fruits
EDIT: I know I said I was not going to case it, but I I cased the non h2o2 one so I guess I had to 
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Edited by Juke Adro (07/03/08 09:18 PM)
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Juke Adro
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Re: h202 discovery [Re: Juke Adro]
#8599081 - 07/05/08 10:54 PM (15 years, 10 months ago) |
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Sorry guys the experiment is over,
all my work was lost (not to any contam) other reasons 
I will consider doing it again in the next week or so
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robanero
β Lib


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Re: h202 discovery [Re: Juke Adro]
#8600443 - 07/06/08 11:20 AM (15 years, 10 months ago) |
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Sorry you lost them. It was still very interesting to follow. I hope you or someone else,Tripsis, will continue this and post results. Happy growing.
--------------------
  310 open cap Hawaiians grown a 1 pint WBSF cake. SPAWN RATIO CALCULATOR My Little Hawaiians Spawn Bag Tek
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cutemushie
Mushrooms are fascinating.



Registered: 05/31/08
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Quote:
RogerRabbit said: We specifically use small gas exchange holes in our jar lids to keep CO2 at a high level during colonization. You don't want lots of O2. The reason is to prevent the mycelium from consuming too much of the substrate prior to fruiting.
Increased O2 is a major pinning trigger. If you colonzize the jars in a high O2 environment, not only does the mycelium consume more of the substrate that you want to save for fruiting, you also remove a major pinning trigger when you move the substrates to your fruiting environment. It will be interesting to see how these fruit, how many flushes, etc. RR
What about using it in the fruiting chamber, say totally enclosed fruiting chamber? Would the increased O2 be beneficial to the mushrooms? Would it cause less contamination on the casing due to the increased O2? Is the level of increased O2 significant? What about adding a little bleach to the peroxide since it will speed up decomposition? I think what RR was referring to is that O2 does increase mycelium growth just like aeration in LC but it is not a good thing during spawn run. But for fruiting, would it be positive?
If the above works for spawn run, how about applying it to agar plates chamber to help mycelium outgrow contam?
I also read somewhere that RR mentioned that frequent air exchanges will prevent trich spores from germinating. But the substrate is full of mini pores, especially if it is loose. Maybe they won't germinate or sporulate on the surface but will they germinate subsurface and the resulting trich mycelium eats up the mushroom mycelium even without the trich sporulating? Any comments/answers? Thanks.
-------------------- HAVE: Paneolus cyanescence, Paneolus cambodgiensis, Psilocybe cubensis, Flammulina velutipe (white, and brown Korean strain), Hericium erinaceous, Coprinus atramentarius, Coprinus comatus, Agrocybe aegerita, Pleurotus eryngii, Psilocybe subaeruginosa, Ganoderma lucidum, Pleurotus nebrodensis, Lepista nuda, Agaricus augustus, Pleurotus cystidiosus, Volvariella speciosa. LOOKING FOR: Those that I don't have.
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The Cake Guy
Bad Ass



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All that hard work and bam its over. I guess thats what this is all about though. You can't get any where without trying. Good job and keep it going.
--------------------

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Juke Adro
I love peach fluff


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Yeah it sucks mate, I will be doing it again within the next month or so, I really wanted to see the end result
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AcidHorse
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Quote:
RogerRabbit said: Many growers have placed peroxide in perlite and humidifiers, but with no increased growth. To test peroxide, I once brought home an air analyzer from work and tested the O2 content of the air, then added 35% industrial grade peroxide to the humidifier(no water). There was no noticeable increase in O2 levels in the fruiting chamber.
It actually sounds more like a coincidence. However, increased oxygen during colonization would not necessarily be a good thing. We use small gas exchange holes in our lids precisely to keep the CO2 levels high during colonization. Doing so prevents the mycelium from consuming too much of the substrate during this period. Increasing the O2 content of the air is one of the main pinning triggers, and during the jar colonization stage, you don't want to introduce pinning triggers. RR
Roger if you put it in a humidifier where no light could get to it or in a container where no light could get to it, it will not break down to oxygen, ..."Because it breaks down quickly when exposed to light, it should be stored in an opaque container, and pharmaceutical formulations typically come in brown bottles that filter out light" The rate of decomposition is dependent on the temperature and concentration of the peroxide, as well as the pH and the presence of impurities and stabilizers.
Sounds handy for oxygen needs, but with shrooms like shiitake they'll need CO2 which I wonder if a sodium bicarbonate and acetic acid reaction would create enough CO2?
-------------------- If you wanna ride, don't ride the white horse, if you wanna ride, ride the white pony
Edited by AcidHorse (09/30/08 06:14 PM)
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AcidHorse
No Name No Slogan



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Re: h202 discovery [Re: poof]
#9009818 - 09/30/08 06:23 PM (15 years, 7 months ago) |
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Quote:
poof said: ok you found something great that works for you, whats your point in trying to convince these know-it-all losers that it works? hell, if i were you i would have kept it to myself just so others could fail harder than they already do.
they are democrats and not fascist capitalistic corporate proprietary morons.
-------------------- If you wanna ride, don't ride the white horse, if you wanna ride, ride the white pony
Edited by AcidHorse (09/30/08 06:27 PM)
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AcidHorse
No Name No Slogan



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Re: h202 discovery [Re: Juke Adro]
#9009885 - 09/30/08 06:34 PM (15 years, 7 months ago) |
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One of the reasons I think the incubator actually may have released O2 is because in some incubators they have a constant light source, which would contribute to the decomposition of the H2O2.
-------------------- If you wanna ride, don't ride the white horse, if you wanna ride, ride the white pony
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Juke Adro
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Re: h202 discovery [Re: AcidHorse]
#9010031 - 09/30/08 07:07 PM (15 years, 7 months ago) |
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Project was never finished and until then the works are void you can keep on thinking about how it worked and why but unless its tested again no one will ever really ever know, I never used a light.
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denger
Mycelium keeper



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Re: h202 discovery [Re: Juke Adro]
#9010931 - 09/30/08 10:09 PM (15 years, 7 months ago) |
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Have anyone tried calculating how much oxygen can be released from 10-20ml of 3% H2O2? I did, and I encourage you to as well. Once you do it yourself, and then shrug in disbelief and re-calculate a few times, you will realize how ridiculous the whole notion of supplementing oxygen with H2O2 is. I am not saying that H2O2 cannot have an effect on mycelium growth, or on fruit body formation by some other mechanism, but it sure as hell is not because of additional O2. I guarantee!
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Juke Adro
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Re: h202 discovery [Re: denger]
#9010962 - 09/30/08 10:17 PM (15 years, 7 months ago) |
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Yeah I agree but it does do something, weather its for the good or bad I don't know lol
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JaComet
Old Hand

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Re: h202 discovery [Re: denger]
#9012104 - 10/01/08 05:40 AM (15 years, 7 months ago) |
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H2O2 releases 3.3 volumes of oxygen for every 1% of solution strength. For example, you may find 3% solutions referred to as “10 Volume”.
--------------------
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Juke Adro
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Re: h202 discovery [Re: JaComet]
#9019530 - 10/02/08 05:56 PM (15 years, 7 months ago) |
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So mine was 165 volume?
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JaComet
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Re: h202 discovery [Re: Juke Adro]
#9022322 - 10/03/08 05:24 AM (15 years, 7 months ago) |
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I was not familiar with your product , so I looked up Oxy Plus and presume you are using Growth Technology Oxy Plus.
Appears to contains 17.5% Hydrogen Peroxide. I suppose they would use 50% solution in their formulation. I use 35% Food Grade at home.
--------------------
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Juke Adro
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Re: h202 discovery [Re: JaComet]
#9023931 - 10/03/08 02:30 PM (15 years, 7 months ago) |
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The old one is 17.5% I used to buy that also, but the new one is 50%
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JaComet
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Re: h202 discovery [Re: Juke Adro]
#9031277 - 10/05/08 05:16 AM (15 years, 7 months ago) |
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50%! Is the product to be stored in a freezer? The fumes off that stuff at room temp must be pretty rude. Being careful now.
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Cultivate

Registered: 03/29/08
Posts: 155
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*DELETED* [Re: JaComet]
#9034127 - 10/05/08 07:25 PM (15 years, 7 months ago) |
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Post deleted by Cultivate<p>Reason for deletion: .
Edited by Cultivate (09/19/10 10:29 AM)
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JaComet
Old Hand

Registered: 11/12/02
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Re: h202 discovery [Re: Cultivate]
#9035771 - 10/06/08 04:11 AM (15 years, 7 months ago) |
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Perhaps “fumes” was a poor choice of words. I take precaution when opening a bottle of 35%, fresh from the freezer, as the vapors bleach my skin to mild irritation. Wouldn’t want anyone to get this stuff on them.
I know I should wear gloves but am simply unable to work in them safely. At least where I feel confident nothing will slip.
I’m very surprised 50% solution is sold in ( ahem ) Garden Shops. Is this stuff even kept in refrigerators, at least? I wonder what the stabilizer is?
--------------------
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Juke Adro
I love peach fluff


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Re: h202 discovery [Re: JaComet]
#9037804 - 10/06/08 03:11 PM (15 years, 7 months ago) |
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I keep it in the garage its always cool in their, like I said in a prior post it is not a normal garden shop its a hydroponic shop that specialises in large scale equipment and chemicals for farms and he is a friend of mine.
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Cultivate

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Re: h202 discovery [Re: Juke Adro]
#9063756 - 10/11/08 04:02 PM (15 years, 7 months ago) |
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Gloves are a good idea. I personally wouldn't go near that stuff without some eye protection.
Can you imagine slipping and getting some of that in the eyes?
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makaveli8x8
Stranger


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Re: h202 discovery [Re: Cultivate]
#9065450 - 10/12/08 12:44 AM (15 years, 7 months ago) |
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or if your drunk and grab the h2o2 instead of the h2o
--------------------
  We were sent to hell for eternity Ø h® We play on earth to pass the time Over-population the root of all Evil-brings the Elites Closer to the gates.
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tripchip
The Mushroom Monkey




Registered: 07/07/08
Posts: 1,154
Loc: African Jungle
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Heres an idea, if you had a martha setup couldnt you put a small glass of peroxide inside the fruiting chamber on one of the shelves to give the martha an extra oxygen boost? or wouldnt it be a good idea for people that have FAE problems to add some in their chamber? for instance some people have a hard time getting high humidity with optimal FAE. The more FAE you have the harder it is to have high humidity. with this said I think people lack the FAE by trying to keep their humidity high. So Im thinking that adding peroxide to your perlite may help balance the best of both world in some cases. not as a substitute for FAE but more like a supplement. PLUS!!, if the O2 is a pinning trigger wouldnt the h202 help as a pinning trigger? I just read this thread for the first time and I find it interesting. I agree with RR as far as this particular experiment but this thread opened my mind to other benificial uses with h202. Im thinking when you want to trigger pinning the h2o2 may really help give the myc get a firm message to begin to pin which may help with better pinsets.. sorry i kinda rambled on i got a lot of things going on in my head and having ADHD doesnt help
-------------------- Psilocybe Cubensis Psilocybe comes from the Greek root "psilos" meaning bald head and Cubensis because it was first recognized as a new species from specimens collected in Cuba. CHIP
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makaveli8x8
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Re: h202 discovery [Re: tripchip]
#9085218 - 10/16/08 05:07 AM (15 years, 7 months ago) |
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i just thought of one possible problem, since h2o2 is an irratant to the skin. using it for humidity purposes could be very dangerous to your lungs i came up with this thought after thinking about using h2o2 with a ultra sonic fogger. but in the end it would be way to expensive to use the stuff anyway...what about 4 bottles a day for a couple weeks, yikes thats alot of money even if you used half that much.
--------------------
  We were sent to hell for eternity Ø h® We play on earth to pass the time Over-population the root of all Evil-brings the Elites Closer to the gates.
Edited by makaveli8x8 (10/16/08 05:09 AM)
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JaComet
Old Hand

Registered: 11/12/02
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Peroxide should be used sparingly. In nature H2O2 concentrations are just a few hundred PPM at best. The highest concentration for therapeutic use is 0.05%. ( or is it 0.5%. I’ll need to look that up to be certain )
It must be remembered that H2O2 releases highly oxidizing singlet Oxygen, not atmospheric triplet. Here’s a quick overview.
http://www.answers.com/topic/singlet-oxygen http://www.answers.com/topic/triplet-oxygen
Would not be so good for mushroom humidity in high concentrations. Also note, un-stabilized products attack plastics. Maybe not so good for fogger diaphragms.
I use a little as a matter of course to precondition my well water and reduce overall bacterial load. About one teaspoon of household per pint of spray water maximum. For Food Grade 35%, I use 4 to 5 drops. Would consider the same ratios for humidifier / fogger use.
--------------------
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MycoAu
5thKingdomCome


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Re: h202 discovery [Re: JaComet]
#9091565 - 10/17/08 12:41 PM (15 years, 7 months ago) |
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While I agree with not utilizing peroxide in high concentrations for your experiments, it does have therapeutic uses as high as 3% as an injectable solution! This is highest I've seen though, and I'm not a huge fan of this particular use due to the nature of its capability to produce oxidative stress in the body.
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JaComet
Old Hand

Registered: 11/12/02
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Re: h202 discovery [Re: MycoAu]
#9095265 - 10/18/08 04:47 AM (15 years, 6 months ago) |
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I’ll stand corrected.
I don’t recall 3% being used IV. Maybe that’s where the embolism deaths came from.(?) I’ll have to hit the books.
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Juke Adro
I love peach fluff


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Re: h202 discovery [Re: JaComet]
#9102866 - 10/20/08 03:51 AM (15 years, 6 months ago) |
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Just for everyones information I would like to say that the test is back on, this time I'll fruit them and see how we go, no doubt that things are going to be worse in the h2o2 trays but I have to finish it for my own record
Pictures will start tomorrow night, I have already stared ten jars all up 5 regular way and the other 5 h2o2'd up, jars are isolated clones so no piss farting around.
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Revival
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Re: h202 discovery [Re: Juke Adro]
#9114588 - 10/22/08 09:12 AM (15 years, 6 months ago) |
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man im very excited for this. I just finished reading through this whole thread and i am very interested. +1 Man keep going!! -Revival
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Cultivate

Registered: 03/29/08
Posts: 155
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Re: h202 discovery *DELETED* [Re: Revival]
#9127488 - 10/24/08 03:19 PM (15 years, 6 months ago) |
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Post deleted by CultivateReason for deletion: .
Edited by Cultivate (10/25/08 03:11 PM)
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jinxedsoul
It's all in yourhead.



Registered: 03/21/08
Posts: 81
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Re: h202 discovery [Re: Cultivate]
#9129310 - 10/24/08 10:40 PM (15 years, 6 months ago) |
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If you really wanted to do it right wouldn't you be using the same isolated strain for all conditions. If im not mistaken you said it was all multi-spore? multi-spore isn't exactly the best for a scientific experiment.
Not to discurge your or anything... Thing that intrigues me the most about all this would be the benefit of getting 80-100% colonization before contaminants have a chance to take hold.
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Juke Adro
I love peach fluff


Registered: 04/05/08
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Quote:
jinxedsoul said: If you really wanted to do it right wouldn't you be using the same isolated strain for all conditions. If im not mistaken you said it was all multi-spore? multi-spore isn't exactly the best for a scientific experiment.
Not to discurge your or anything... Thing that intrigues me the most about all this would be the benefit of getting 80-100% colonization before contaminants have a chance to take hold.
I am I just have had no time to take pictures but its coming along nice
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Revival
Stranger

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Re: h202 discovery [Re: Juke Adro]
#9136135 - 10/26/08 10:36 AM (15 years, 6 months ago) |
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Juke Adro
I love peach fluff


Registered: 04/05/08
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Re: h202 discovery [Re: Revival]
#9142476 - 10/27/08 03:04 PM (15 years, 6 months ago) |
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Tomorrow I promise
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tripchip
The Mushroom Monkey




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Re: h202 discovery [Re: Juke Adro]
#9181291 - 11/04/08 06:43 AM (15 years, 6 months ago) |
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Quote:
Juke Adro said: Tomorrow I promise
7 days later..... (JK)
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Juke Adro
I love peach fluff


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Re: h202 discovery [Re: tripchip]
#9189445 - 11/05/08 02:42 PM (15 years, 6 months ago) |
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HAHAHA I been busy with http://www.shroomery.org/forums/showflat.php/Cat/0/Number/9074230/an/0/page/0/gonew/1#UNREAD I swear I have like 50 dishes and so many jars its not funny its draining my life.
H2O2 is also going but as I'm at work I cant post any pics atm but the h202 jars are already spawned to manure
so far it seems the same as last time
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ghiajake
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Re: h202 discovery (NEW HIGH RES PICS UPDATED) (NEW UPDATE 17-6-08) [Re: Juke Adro]
#17532719 - 01/11/13 01:21 PM (11 years, 4 months ago) |
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After reading the majority of the reply's I have a few thoughts to share. Is it possible that the oxygen rich atmosphere helps keep CO2 levels high in the jars while providing enough oxygen to feed on. Since CO2 is heavier than oxygen (room air is only 20% oxygen btw yall), it seems plausible to me that the oxygen would be fed into the jars as needed (once CO2 is broke down and space is available) while utilizing the CO2 more efficient than otherwise. I would imagine the reason you have such prolific growth at a lower incubation temp is due to atmospheric pressure in the chamber, and the heat that the CO2 produces in the jars. Your jars are probably drying out due to the hardy growth. Have you tried spawning bulk media in this environment? Dung, or something that can hold a bit more internal moisture? I have wondered what the effects would be on mycelia and fruiting in a pressurized, controlled ratio O2/CO2 rich environment using air tanks. Its interesting that you stumbled upon an easy way of testing it. Just inoculated some jars yesterday with spores, so I'll try it out now. I'll start a thread on my grow. Now, on to the task of scientific exploration!
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cloudpersona
I don't even...

Registered: 12/17/11
Posts: 1,285
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Re: h202 discovery (NEW HIGH RES PICS UPDATED) (NEW UPDATE 17-6-08) [Re: ghiajake]
#17533770 - 01/11/13 04:54 PM (11 years, 4 months ago) |
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4 Year old thread bro.
-------------------- “The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.” – Terence McKenna “If the words ‘life, liberty, and the pursuit of happiness’ don’t include the right to experiment with your own consciousness, then the Declaration of Independence isn’t worth the hemp it was written on.” -Terence McKenna
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Juke Adro
I love peach fluff


Registered: 04/05/08
Posts: 6,957
Loc: Inside your head
Last seen: 5 years, 1 month
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Re: h202 discovery (NEW HIGH RES PICS UPDATED) (NEW UPDATE 17-6-08) [Re: ghiajake]
#17533885 - 01/11/13 05:18 PM (11 years, 4 months ago) |
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Hey mate, it may be 4 years old but it shows you are doing the right thing and searching before posting and that deserves credit. After this thread i kept using h2o2 but never on any substrate just as a cleaning agent and in the incubator. By all means try what ever makes you happy and good luck with your endeavors
-------------------- Someone said: im actually not using ms, im using prints.
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