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InvisibleJaComet
Old Hand

Registered: 11/12/02
Posts: 347
Loc: Out Yonder
Re: h202 discovery [Re: makaveli8x8]
    #9090325 - 10/17/08 06:00 AM (15 years, 7 months ago)

Peroxide should be used sparingly. In nature H2O2 concentrations are just a few hundred PPM at best. The highest concentration for therapeutic use is  0.05%. ( or is it 0.5%. I’ll need to look that up to be certain )

It must be remembered that H2O2 releases highly oxidizing singlet Oxygen, not atmospheric triplet. Here’s a quick overview.

http://www.answers.com/topic/singlet-oxygen
http://www.answers.com/topic/triplet-oxygen

Would not be so good for mushroom humidity in high concentrations.  Also note, un-stabilized products attack plastics. Maybe not so good for fogger diaphragms.

I use a little as a matter of course to precondition my well water and reduce overall bacterial load. About one teaspoon of household per pint of spray water maximum. For Food Grade 35%, I use 4 to 5 drops. Would consider the same ratios for humidifier / fogger use.


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OfflineMycoAu
5thKingdomCome
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Registered: 07/18/07
Posts: 1,047
Last seen: 3 years, 4 months
Trusted Cultivator
Re: h202 discovery [Re: JaComet]
    #9091565 - 10/17/08 12:41 PM (15 years, 7 months ago)

While I agree with not utilizing peroxide in high concentrations for your experiments, it does have therapeutic uses as high as 3% as an injectable solution!  This is highest I've seen though, and I'm not a huge fan of this particular use due to the nature of its capability to produce oxidative stress in the body.

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InvisibleJaComet
Old Hand

Registered: 11/12/02
Posts: 347
Loc: Out Yonder
Re: h202 discovery [Re: MycoAu]
    #9095265 - 10/18/08 04:47 AM (15 years, 6 months ago)

I’ll stand corrected.

I don’t recall 3% being used IV. Maybe that’s where the embolism deaths came from.(?) I’ll have to hit the books.


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OfflineJuke Adro
I love peach fluff
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Registered: 04/05/08
Posts: 6,957
Loc: Inside your head
Last seen: 5 years, 1 month
Re: h202 discovery [Re: JaComet]
    #9102866 - 10/20/08 03:51 AM (15 years, 6 months ago)

Just for everyones information I would like to say that the test is back on, this time I'll fruit them and see how we go, no doubt that things are going to be worse in the h2o2 trays but I have to finish it for my own record :wink:

Pictures will start tomorrow night, I have already stared :wink:
ten jars all up 5 regular way and the other 5 h2o2'd up, jars are isolated clones so no piss farting around.


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Someone said:  im actually not using ms, im using prints.
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OfflineRevival
Stranger

Registered: 10/19/08
Posts: 11
Last seen: 15 years, 6 months
Re: h202 discovery [Re: Juke Adro]
    #9114588 - 10/22/08 09:12 AM (15 years, 6 months ago)

man im very excited for this. I just finished reading through this whole thread and i am very interested. +1 Man keep going!!
-Revival

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InvisibleCultivate

Registered: 03/29/08
Posts: 155
Re: h202 discovery *DELETED* [Re: Revival]
    #9127488 - 10/24/08 03:19 PM (15 years, 6 months ago)

Post deleted by Cultivate

Reason for deletion: .


Edited by Cultivate (10/25/08 03:11 PM)

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Invisiblejinxedsoul
It's all in yourhead.
Male User Gallery


Registered: 03/21/08
Posts: 81
Re: h202 discovery [Re: Cultivate]
    #9129310 - 10/24/08 10:40 PM (15 years, 6 months ago)

If you really wanted to do it right wouldn't you be using the same isolated strain for all conditions. If im not mistaken you said it was all multi-spore? multi-spore isn't exactly the best for a scientific experiment.

Not to discurge your or anything...
Thing that intrigues me the most about all this would be the benefit of getting 80-100% colonization before contaminants have a chance to take hold.

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OfflineJuke Adro
I love peach fluff
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Registered: 04/05/08
Posts: 6,957
Loc: Inside your head
Last seen: 5 years, 1 month
Re: h202 discovery [Re: jinxedsoul]
    #9132217 - 10/25/08 02:44 PM (15 years, 6 months ago)

Quote:

jinxedsoul said:
If you really wanted to do it right wouldn't you be using the same isolated strain for all conditions. If im not mistaken you said it was all multi-spore? multi-spore isn't exactly the best for a scientific experiment.

Not to discurge your or anything...
Thing that intrigues me the most about all this would be the benefit of getting 80-100% colonization before contaminants have a chance to take hold.




I am :wink: I just have had no time to take pictures but its coming along nice


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OfflineRevival
Stranger

Registered: 10/19/08
Posts: 11
Last seen: 15 years, 6 months
Re: h202 discovery [Re: Juke Adro]
    #9136135 - 10/26/08 10:36 AM (15 years, 6 months ago)

yay :smile: :dancer: UPDATES :smile:
-Rev

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OfflineJuke Adro
I love peach fluff
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Registered: 04/05/08
Posts: 6,957
Loc: Inside your head
Last seen: 5 years, 1 month
Re: h202 discovery [Re: Revival]
    #9142476 - 10/27/08 03:04 PM (15 years, 6 months ago)

Tomorrow I promise :wink:


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Invisibletripchip
The Mushroom Monkey
Female User Gallery


Folding@home Statistics
Registered: 07/07/08
Posts: 1,154
Loc: African Jungle
Re: h202 discovery [Re: Juke Adro]
    #9181291 - 11/04/08 06:43 AM (15 years, 6 months ago)

Quote:

Juke Adro said:
Tomorrow I promise :wink:




7 days later.....:rolleyes:  (JK)

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OfflineJuke Adro
I love peach fluff
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Registered: 04/05/08
Posts: 6,957
Loc: Inside your head
Last seen: 5 years, 1 month
Re: h202 discovery [Re: tripchip]
    #9189445 - 11/05/08 02:42 PM (15 years, 6 months ago)

HAHAHA I been busy with http://www.shroomery.org/forums/showflat.php/Cat/0/Number/9074230/an/0/page/0/gonew/1#UNREAD I swear I have like 50 dishes and so many jars its not funny its draining my life.

H2O2 is also going but as I'm at work I cant post any pics atm but the h202 jars are already spawned to manure :wink:

so far it seems the same as last time


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Offlineghiajake
Myco-Viking
Male

Registered: 01/10/13
Posts: 3,897
Loc: Indiana
Last seen: 1 day, 1 hour
Re: h202 discovery (NEW HIGH RES PICS UPDATED) (NEW UPDATE 17-6-08) [Re: Juke Adro]
    #17532719 - 01/11/13 01:21 PM (11 years, 4 months ago)

After reading the majority of the reply's I have a few thoughts to share. Is it possible that the oxygen rich atmosphere helps keep CO2 levels high in the jars while providing enough oxygen to feed on. Since CO2 is heavier than oxygen (room air is only 20% oxygen btw yall), it seems plausible to me that the oxygen would be fed into the jars as needed (once CO2 is broke down and space is available) while utilizing the CO2 more efficient than otherwise. I would imagine the reason you have such prolific growth at a lower incubation temp is due to atmospheric pressure in the chamber, and the heat that the CO2 produces in the jars. Your jars are probably drying out due to the hardy growth. Have you tried spawning bulk media in this environment? Dung, or something that can hold a bit more internal moisture? I have wondered what the effects would be on mycelia and fruiting in a pressurized, controlled ratio O2/CO2 rich environment using air tanks. Its interesting that you stumbled upon an easy way of testing it. Just inoculated some jars yesterday with spores, so I'll try it out now. I'll start a thread on my grow. Now, on to the task of scientific exploration! :wink:

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Offlinecloudpersona
I don't even...

Registered: 12/17/11
Posts: 1,285
Last seen: 6 years, 6 months
Re: h202 discovery (NEW HIGH RES PICS UPDATED) (NEW UPDATE 17-6-08) [Re: ghiajake]
    #17533770 - 01/11/13 04:54 PM (11 years, 4 months ago)

4 Year old thread bro.


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“The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.”
– Terence McKenna
“If the words ‘life, liberty, and the pursuit of happiness’ don’t include the right to experiment with your own consciousness, then the Declaration of Independence isn’t worth the hemp it was written on.”
-Terence McKenna

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OfflineJuke Adro
I love peach fluff
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Registered: 04/05/08
Posts: 6,957
Loc: Inside your head
Last seen: 5 years, 1 month
Re: h202 discovery (NEW HIGH RES PICS UPDATED) (NEW UPDATE 17-6-08) [Re: ghiajake]
    #17533885 - 01/11/13 05:18 PM (11 years, 4 months ago)

Hey mate, it may be 4 years old but it shows you are doing the right thing and searching before posting and that deserves credit.
After this thread i kept using h2o2 but never on any substrate just as a cleaning agent and in the incubator.
By all means try what ever makes you happy and good luck with your endeavors


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