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Invisiblewisp

Registered: 04/13/08
Posts: 5,304
Re: h202 discovery [Re: flavoraid]
    #8547255 - 06/21/08 11:33 AM (15 years, 10 months ago)

It is more aggressive undoubtedly. But still, on the plates with H2O2 the Orissa did better than on the plates without. This is by no means a controlled experiment, just an observation.

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OfflineJuke Adro
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Registered: 04/05/08
Posts: 6,957
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Re: h202 discovery [Re: wisp]
    #8553154 - 06/23/08 04:45 AM (15 years, 10 months ago)

I will take the foil off tonight and take a pic of how the spawned poo looks i will post some pics in a day or 2....lets see if its ready or not to be fruited.


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Someone said:  im actually not using ms, im using prints.
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InvisibleMushHunter08
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Registered: 06/08/08
Posts: 522
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Re: h202 discovery [Re: Juke Adro]
    #8558595 - 06/24/08 02:25 PM (15 years, 10 months ago)

Come on with those pics man. This is a very interesting thread to me.


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"The road to excess leads to the palace of wisdom...for we never know what is enough until we know what is more than enough."
-William Blake-

The most simple method of growing mushrooms:
www.mushroomvideos.com

MultiSync's lazy bastard print/syringe guide

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InvisibleBlimeyGrimey
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Re: h202 discovery [Re: blackout]
    #8558608 - 06/24/08 02:29 PM (15 years, 10 months ago)

Quote:

blackout said:
People could be in a rush, going away on holidays etc. Many will toss grows that could get another small flush from, not being worth their while.




Its always best to bury spent cakes in the yard or garden or even throw them in a compost pile. Throwing them in the trash is a complete waste.


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Message me for free microscopy services on Psilocybe, Panaeolus, and Gymnopilus species.

Looking for wild Panaeolus cinctulus and Panaeolus olivaceus prints.

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OfflineJuke Adro
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Registered: 04/05/08
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Re: h202 discovery [Re: BlimeyGrimey]
    #8565602 - 06/26/08 06:29 AM (15 years, 10 months ago)

Ok sorry about how it took so long but the news is that the spawn stalled :frown: and the long awaited answer is that..... the first two of my test must have been luck
it seems that the h2o2 grain is a no go it may colonise fast as hell but it just wont continue to grow the poo i used is prime and it just wont take....

on the other hand the ones that were not exposed to a stupid amount of h2o2 have been spawned to the same shit and already fully colonised it and have been cased and its popping through and is 2 days off being fruited.

so there you go :frown:

I will post pics tomorrow same time same place I promice, I'm at work atm sorry guys

thanks for watching


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Someone said:  im actually not using ms, im using prints.
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Invisiblewisp

Registered: 04/13/08
Posts: 5,304
Re: h202 discovery [Re: Juke Adro]
    #8565662 - 06/26/08 07:12 AM (15 years, 10 months ago)

Ah well, can't say you didn't try. At least you gave it a proper go and wasn't daunted by other peoples doubts.:thumbup:

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OfflineJuke Adro
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Re: h202 discovery [Re: wisp]
    #8565677 - 06/26/08 07:21 AM (15 years, 10 months ago)

I cant believe it lol it was so promising I suppose the myc had enough it will still fruit one day but just not anytime soon

should listened to RR 120 posts back ha ha ha oh well


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Someone said:  im actually not using ms, im using prints.
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Invisiblewisp

Registered: 04/13/08
Posts: 5,304
Re: h202 discovery [Re: Juke Adro]
    #8565681 - 06/26/08 07:27 AM (15 years, 10 months ago)

:lol:

Well it wouldn't be science with out testing. No point in believing everything you are told. Experience is the best way to learn.

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Offlinemakaveli8x8
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Re: h202 discovery [Re: Juke Adro]
    #8565702 - 06/26/08 07:38 AM (15 years, 10 months ago)

hmm so your saying it grew fine until you spawned it...your spawning containers where are they?

i ask because, the jars that were around the h2o2, if they were indeed growing quicker due to the h2o2, purhaps they became selective to it.  in other words they depended on it, and were producing certain emzemes to bread it down to speed up there growth, and when you removed that growth facter they were stuck without it.

so if your poo trays arn't near the h2o2 move them back?  or if thats where they already are just ignore my ramblings :lol:


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We were sent to hell for eternity :hellfire: Ø:omgawesome:h®
We play on earth to pass the time :foreheadslap:

Over-population the root of all Evil-brings the Elites Closer to the gates.

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Invisiblewisp

Registered: 04/13/08
Posts: 5,304
Re: h202 discovery [Re: makaveli8x8]
    #8565707 - 06/26/08 07:40 AM (15 years, 10 months ago)

Junkie fungi huh?:grin:

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OfflineJuke Adro
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Re: h202 discovery [Re: makaveli8x8]
    #8565728 - 06/26/08 07:51 AM (15 years, 10 months ago)

Quote:

makaveli8x8 said:
hmm so your saying it grew fine until you spawned it...your spawning containers where are they?

i ask because, the jars that were around the h2o2, if they were indeed growing quicker due to the h2o2, purhaps they became selective to it.  in other words they depended on it, and were producing certain emzemes to bread it down to speed up there growth, and when you removed that growth facter they were stuck without it.

so if your poo trays arn't near the h2o2 move them back?  or if thats where they already are just ignore my ramblings :lol:




yeah it was with h2o2 the whole time, so i think it just changed to fruit mode????


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Someone said:  im actually not using ms, im using prints.
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Offlineblackout
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Re: h202 discovery [Re: wisp]
    #8566704 - 06/26/08 01:09 PM (15 years, 10 months ago)

Quote:

tripsis said:
No point in believing everything you are told. Experience is the best way to learn.



Exactly, a lot of stuff people do in all sorts of hobbies and activities would have been laughed at, or told not to bother with at one stage.

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OfflineJuke Adro
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Re: h202 discovery [Re: blackout]
    #8570024 - 06/27/08 06:26 AM (15 years, 10 months ago)

First pic is the h2o2, second is non h2o2...the second one fully colonised the poo and is now cased in 50/50 and gypsum mix and is popping through already.

the h2o2 is still colonising but very very slow, I will fruit it when ever it decides to finish colonising the horse poo, I'm not going to case it or we will be here till Christmas lol i will update every few days :wink:





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InvisibleMushHunter08
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Registered: 06/08/08
Posts: 522
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Re: h202 discovery [Re: Juke Adro]
    #8584251 - 07/01/08 02:35 PM (15 years, 10 months ago)

Hey Juke Adro, when you don't get what you want. Experience is what you get. I think it was fantastic that you were so hard headed and continued the experiment though. Even though RR was right. Nice job anyway brother! :grin:


--------------------
"The road to excess leads to the palace of wisdom...for we never know what is enough until we know what is more than enough."
-William Blake-

The most simple method of growing mushrooms:
www.mushroomvideos.com

MultiSync's lazy bastard print/syringe guide

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OfflineSeussA
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Re: h202 discovery [Re: Juke Adro]
    #8586832 - 07/02/08 06:00 AM (15 years, 10 months ago)

>  and the long awaited answer is that..... the first two of my test must have been luck

I don't buy it (luck that is).  In science, failures are just as important (and often more important) than successes.  Form a new hypothesis to test to figure out why your first grow was so much better.  Something has to be different... we are fairly certain it isn't the peroxide, based upon you testing, so what else could it be?


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Just another spore in the wind.

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Invisibleflavoraid
now with twicethe ketamine andopiates!
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Registered: 12/05/07
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Re: h202 discovery [Re: MushHunter08]
    #8586839 - 07/02/08 06:07 AM (15 years, 10 months ago)

Quote:

MushHunter08 said:
Hey Juke Adro, when you don't get what you want. Experience is what you get. I think it was fantastic that you were so hard headed and continued the experiment though. Even though RR was right. Nice job anyway brother! :grin:




agreed.

Experimenting is needed for mycology to progress.


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coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.

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OfflineJuke Adro
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Re: h202 discovery [Re: flavoraid]
    #8592780 - 07/03/08 07:19 PM (15 years, 10 months ago)

Well The first time it was a accident so i was not sure exactly how much peroxide was left in the tub, so I estimated for the second, which was the exact same amount this as in this test, I am not experienced enough to understand what happened or how this time it failed well not failed but is just so slow???

it seems that from inoculation to spawn time its super fast for some reason and without peroxide its normal

update on the h2o2 tub is fully colonised
it is now cased and put in fruiting conditions but I did not take a pic of the casing

Il pop pics up when it fruits and when the non h2o2 fruits

EDIT: I know I said I was not going to case it, but I I cased the non h2o2 one so I guess I had to :wink:



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Edited by Juke Adro (07/03/08 09:18 PM)

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OfflineJuke Adro
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Re: h202 discovery [Re: Juke Adro]
    #8599081 - 07/05/08 10:54 PM (15 years, 10 months ago)

Sorry guys the experiment is over,

all my work was lost (not to any contam) other reasons :frown:

I will consider doing it again in the next week or so


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Offlinerobanero
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Registered: 09/05/06
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Re: h202 discovery [Re: Juke Adro]
    #8600443 - 07/06/08 11:20 AM (15 years, 10 months ago)

Sorry you lost them. It was still very interesting to follow. I hope you or someone else,Tripsis, will continue this and post results. Happy growing.


--------------------

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Offlinecutemushie
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Registered: 05/31/08
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Re: h202 discovery [Re: RogerRabbit]
    #8966739 - 09/22/08 02:41 AM (15 years, 7 months ago)

Quote:

RogerRabbit said:
We specifically use small gas exchange holes in our jar lids to keep CO2 at a high level during colonization.  You don't want lots of O2.  The reason is to prevent the mycelium from consuming too much of the substrate prior to fruiting.

Increased O2 is a major pinning trigger.  If you colonzize the jars in a high O2 environment, not only does the mycelium consume more of the substrate that you want to save for fruiting, you also remove a major pinning trigger when you move the substrates to your fruiting environment.  It will be interesting to see how these fruit, how many flushes, etc.
RR




What about using it in the fruiting chamber, say totally enclosed fruiting chamber?  Would the increased O2 be beneficial to the mushrooms?  Would it cause less contamination on the casing due to the increased O2?  Is the level of increased O2 significant?  What about adding a little bleach to the peroxide since it will speed up decomposition?  I think what RR was referring to is that O2 does increase mycelium growth just like aeration in LC but it is not a good thing during spawn run.  But for fruiting, would it be positive?

If the above works for spawn run, how about applying it to agar plates chamber to help mycelium outgrow contam?

I also read somewhere that RR mentioned that frequent air exchanges will prevent trich spores from germinating.  But the substrate is full of mini pores, especially if it is loose.  Maybe they won't germinate or sporulate on the surface but will they germinate subsurface and the resulting trich mycelium eats up the mushroom mycelium even without the trich sporulating?  Any comments/answers?  Thanks.


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HAVE: Paneolus cyanescence, Paneolus cambodgiensis, Psilocybe cubensis, Flammulina velutipe (white, and brown Korean strain), Hericium erinaceous, Coprinus atramentarius, Coprinus comatus, Agrocybe aegerita, Pleurotus eryngii, Psilocybe subaeruginosa, Ganoderma lucidum, Pleurotus nebrodensis, Lepista nuda, Agaricus augustus, Pleurotus cystidiosus, Volvariella speciosa.

LOOKING FOR: Those that I don't have.

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