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wisp

Registered: 04/13/08
Posts: 5,304
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QuantumReality
Mycopath 🗡



Registered: 05/20/07
Posts: 3,203
Loc: BoobyTraps
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Quote:
legallyhomeless said:
Quote:
Dizzwizzle said: yea just a dust mask will do it.
wearing a dust mask and cleaning wiht 90% ISO is nasty.
True, Better than no mask at all... just dont stick ya head right over where ya cleaning and youll be k
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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 6 months, 11 days
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Quote:
RogerRabbit said:Personally, I think the effects of adding O2 to colonizing jars are deleterious, even if it does speed up colonization. You don't want your substrate being consumed prior to the fruiting stage, so that the nutrients in the substrate are available for the fruitbodies.
Some may value the alleged speeding up more than the decrease in yield. In another thread you mentioned people not being concerned with BE, which is true, grain is cheap.
Homebrewers will aerate their wort prior to pitching yeast, the additional oxygen promotes a fast yeast growth, this ensures a vigorous brew to take off quickly. This speeds up the brew time, and decreases the potential for contams to take hold. Oxygen is then restricted since you do not want the sugars used up making new yeast rather than alcohol.
This could work in a similar way, speed up colonisation so contams have less chance to take hold. You sacrifice some of your substrate to get this.
If something reduced the maturation time of sclerotia to 50%, but lowered the yield to 50% then many might do it.
BTW My grow area is usually only 16C, so I do incubate http://en.wikipedia.org/wiki/Room_temperature Most here tend to be growing in bedrooms, where 18C (64F) is the "recommended room temp". If you want air circulating in a colder room then you could put the jars on electronic devices in standby mode, like dvd players
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MushHunter08
Mycological Pupil



Registered: 06/08/08
Posts: 522
Loc: B.F.E
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Re: h202 discovery [Re: blackout]
#8535125 - 06/17/08 08:24 PM (15 years, 10 months ago) |
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I'm curious to see the results of further testing of this possible variable. I would try it, but not until I see more results for myself.
-------------------- "The road to excess leads to the palace of wisdom...for we never know what is enough until we know what is more than enough." -William Blake- The most simple method of growing mushrooms: www.mushroomvideos.com MultiSync's lazy bastard print/syringe guide
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Juke Adro
I love peach fluff


Registered: 04/05/08
Posts: 6,957
Loc: Inside your head
Last seen: 5 years, 1 month
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I'm just hoping that the juice is worth the squeeze.
-------------------- Someone said: im actually not using ms, im using prints.
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wisp

Registered: 04/13/08
Posts: 5,304
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Re: h202 discovery [Re: Juke Adro]
#8535409 - 06/17/08 10:05 PM (15 years, 10 months ago) |
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Here's an interesting bit of information. I have some petri dishes with Ps. cubensis Orissa strain culture on them. Half are plain MEA agar, the other half H2O2 MEA agar. The ones with the H2O2 are all doing far better than the ones without. This in my experience is quite unusual, the H2O2 usually slows the growth of mycelium markedly but I'm having very different results with this particular strain.
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Juke Adro
I love peach fluff


Registered: 04/05/08
Posts: 6,957
Loc: Inside your head
Last seen: 5 years, 1 month
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Re: h202 discovery [Re: wisp]
#8535423 - 06/17/08 10:09 PM (15 years, 10 months ago) |
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Do you mean h2o2 in your actual MEA or in the TIT with the MEA?
-------------------- Someone said: im actually not using ms, im using prints.
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wisp

Registered: 04/13/08
Posts: 5,304
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Re: h202 discovery [Re: Juke Adro]
#8535453 - 06/17/08 10:19 PM (15 years, 10 months ago) |
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H2O2 in the actual MEA. Different to what you're talking about, I know, but still relevant.
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Juke Adro
I love peach fluff


Registered: 04/05/08
Posts: 6,957
Loc: Inside your head
Last seen: 5 years, 1 month
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Re: h202 discovery [Re: wisp]
#8535458 - 06/17/08 10:21 PM (15 years, 10 months ago) |
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cool I wonder how that works, either way it is good
-------------------- Someone said: im actually not using ms, im using prints.
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wisp

Registered: 04/13/08
Posts: 5,304
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Re: h202 discovery [Re: Juke Adro]
#8535465 - 06/17/08 10:23 PM (15 years, 10 months ago) |
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Yeah I'm not too sure really, it's quite strange. Usually I've had the polar opposite effects as mycelium really doesn't like H2O2.
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LesChanga
Cosmically F'd



Registered: 11/18/06
Posts: 402
Loc: SlimeCounty
Last seen: 15 years, 8 months
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Re: h202 discovery [Re: blackout]
#8537049 - 06/18/08 12:42 PM (15 years, 10 months ago) |
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Quote:
blackout said If something reduced the maturation time of sclerotia to 50%, but lowered the yield to 50% then many might do it.
this is already true... if you reduce the incubation time, you will harvest less sclerotia...
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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 6 months, 11 days
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Re: h202 discovery [Re: wisp]
#8537304 - 06/18/08 02:32 PM (15 years, 10 months ago) |
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Quote:
tripsis said:the H2O2 usually slows the growth of mycelium markedly but I'm having very different results with this particular strain.
If you added it to hot agar it may have degraded quickly turning to oxygen and not having much of a disinfectant/slowing effect.
Edited by blackout (06/18/08 02:39 PM)
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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 6 months, 11 days
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Re: h202 discovery [Re: LesChanga]
#8537316 - 06/18/08 02:38 PM (15 years, 10 months ago) |
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Quote:
LesChanga said:
Quote:
blackout said If something reduced the maturation time of sclerotia to 50%, but lowered the yield to 50% then many might do it.
this is already true... if you reduce the incubation time, you will harvest less sclerotia...
Yep, you got me there! Most say mature sclerotia is stronger though, maybe sclerotia is not the best example. But do you see my point? say your cube grow of 1 kilo of dry grain gave a fresh yield of 1 kilo over 6 weeks. If you could get 1/2 kilo in 3 weeks many might prefer it. People could be in a rush, going away on holidays etc. Many will toss grows that could get another small flush from, not being worth their while.
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Kagenical
Stranger

Registered: 05/27/08
Posts: 128
Last seen: 10 years, 6 months
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Just figured I'd chime in here... I had 3 jars that were just *not* colonizing fully... They smelled fine, and showed no signs of bacterial contamination... They wouldn't colonize the bottom even after I flipped them upside down.
I added 100ml of H2o2 into the bottom of a small Tupperware container (about 1cm up the jars), and put them in it, and closed the lid...
Came back 3 days later to find that one had fully colonized... And two hadn't moved at all.
Unforuntately, one of the ones that hadn't fully colonized yet pinned invitro (I'd assume from the burst of oxygen and lower temperature due to evaporation of the H2o2 that was covering the bottom of the glass jars.
Not sure what this means, and I didn't bother to read the entire thread, but I figured I'd relay my results.
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abitavenger
3rd gear vtak



Registered: 01/15/08
Posts: 545
Loc: east coast
Last seen: 12 years, 3 months
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Re: h202 discovery [Re: Kagenical]
#8538483 - 06/18/08 08:36 PM (15 years, 10 months ago) |
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interesting thread
this is next on my list of need to trys
-------------------- Karma.
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wisp

Registered: 04/13/08
Posts: 5,304
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Re: h202 discovery [Re: blackout]
#8538921 - 06/18/08 10:48 PM (15 years, 10 months ago) |
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Quote:
blackout said:
Quote:
tripsis said:the H2O2 usually slows the growth of mycelium markedly but I'm having very different results with this particular strain.
If you added it to hot agar it may have degraded quickly turning to oxygen and not having much of a disinfectant/slowing effect.
Nope, that certainly didn't happen. I added it when it was cool enough to handle. I also knocked up a few other dishes with another species and had the polar opposite effect. This culture hates the H2O2 but is growing fine on the agar without, so it definitely has not degraded.
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Juke Adro
I love peach fluff


Registered: 04/05/08
Posts: 6,957
Loc: Inside your head
Last seen: 5 years, 1 month
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Re: h202 discovery [Re: Kagenical]
#8539123 - 06/18/08 11:47 PM (15 years, 10 months ago) |
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Quote:
Kagenical said: Just figured I'd chime in here... I had 3 jars that were just *not* colonizing fully... They smelled fine, and showed no signs of bacterial contamination... They wouldn't colonize the bottom even after I flipped them upside down.
I added 100ml of H2o2 into the bottom of a small Tupperware container (about 1cm up the jars), and put them in it, and closed the lid...
Came back 3 days later to find that one had fully colonized... And two hadn't moved at all.
Unforuntately, one of the ones that hadn't fully colonized yet pinned invitro (I'd assume from the burst of oxygen and lower temperature due to evaporation of the H2o2 that was covering the bottom of the glass jars.
Not sure what this means, and I didn't bother to read the entire thread, but I figured I'd relay my results.
Of course it would have pinned lol but my silly theory is that if you do it from the start the myc gets use to it or whatnot.
what percentage was your h2o2?
-------------------- Someone said: im actually not using ms, im using prints.
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MushHunter08
Mycological Pupil



Registered: 06/08/08
Posts: 522
Loc: B.F.E
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Re: h202 discovery [Re: Juke Adro]
#8540513 - 06/19/08 11:39 AM (15 years, 10 months ago) |
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Tripsis exactly what amount of H2O2 did you add the the petri dish with the Orissa?
-------------------- "The road to excess leads to the palace of wisdom...for we never know what is enough until we know what is more than enough." -William Blake- The most simple method of growing mushrooms: www.mushroomvideos.com MultiSync's lazy bastard print/syringe guide
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wisp

Registered: 04/13/08
Posts: 5,304
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1ml of 3% H2O2 to 150ml of agar. It sounds strange I know, but the Orissa culture seems to love it yet the other species I have going wouldn't touch it and I had to transfer them last night.
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flavoraid
now with twicethe ketamine andopiates!

Registered: 12/05/07
Posts: 1,678
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Re: h202 discovery [Re: wisp]
#8547242 - 06/21/08 11:28 AM (15 years, 10 months ago) |
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prehaps a better idea is your orissa isolation is just more aggresive.
h2o2 attacks living cells.
-------------------- coda said: imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.
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