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OfflineRogerRabbitM
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Re: h202 discovery [Re: wisp]
    #8477521 - 06/02/08 10:54 PM (15 years, 11 months ago)

Quote:

1 cup sit's on the shelf in a room at temps between 14C and 20C




Note that when we say normal room temperature, it's a well defined range. 20C to 25C or 68F to 77F. Google it.

Your jars will still colonize at 20C, but will be slower.
RR


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OfflineJuke Adro
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Re: h202 discovery [Re: RogerRabbit]
    #8477537 - 06/02/08 10:57 PM (15 years, 11 months ago)

well i fucked that up now,

give me till tomorrow morning and i will work out something to keep the temp at that range.


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Invisiblewisp

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Re: h202 discovery [Re: Juke Adro]
    #8477716 - 06/02/08 11:48 PM (15 years, 11 months ago)

The joy of living in Melbourne huh? :rofl:

Do what I do. Get one of those little kambrook heaters with a thermostat. Set it on it's lowest setting and turn it on. Whenever in reaches that temp it switches off, and turns back on when it drops below it. Keeps the room at a pretty steady temp ~ 25C. It should be a small room though.

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OfflineJuke Adro
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Re: h202 discovery [Re: wisp]
    #8480530 - 06/03/08 06:14 PM (15 years, 11 months ago)

its done i just put a heat mat from a reptile cage and its timer in the cupboard i set it and seems to be working great its a big heat mat so should be fine.


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OfflineJuke Adro
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Re: h202 discovery [Re: Juke Adro]
    #8484317 - 06/04/08 05:07 PM (15 years, 11 months ago)

ok they all have growth, I will take pics and post them tonight

A little observation noted, was the substrate in the h2o2 jars seemed to be a little more dry than usual, which is a bad thing but the test continues.

Pics coming tonight/tomorrow


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Offlinexhooliganx
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Re: h202 discovery [Re: Juke Adro]
    #8496099 - 06/07/08 12:10 PM (15 years, 11 months ago)

Quote:

Juke Adro said:
its done i just put a heat mat from a reptile cage and its timer in the cupboard i set it and seems to be working great its a big heat mat so should be fine.




ok so if the test group that is supposed to be in an open room at roomtemp is in a closed cupboard with a heat mat isn't  that more like having it in a wooden incubator?


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OfflineJuke Adro
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Re: h202 discovery [Re: xhooliganx]
    #8496442 - 06/07/08 02:28 PM (15 years, 11 months ago)

have you seen my cupboards its a walk in wardrobe so its huge and i walk in and out of it everyday its on the top shelf and gets heaps of air, also the wardrobe is prolly as big as a small room so its fine and that one is not going the best either as i suspected :wink:


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Offlinexhooliganx
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Re: h202 discovery [Re: Juke Adro]
    #8497098 - 06/07/08 06:58 PM (15 years, 11 months ago)

Quote:

Juke Adro said:
have you seen my cupboards its a walk in wardrobe so its huge and i walk in and out of it everyday its on the top shelf and gets heaps of air, also the wardrobe is prolly as big as a small room so its fine and that one is not going the best either as i suspected :wink:



oh I thought you meant a small cupboard because I didn't think a reptile heating matt woul bring the air temp in a small room up 5 C.


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OfflineJuke Adro
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Re: h202 discovery [Re: xhooliganx]
    #8497114 - 06/07/08 07:06 PM (15 years, 11 months ago)

the heat mat is coiled around the glass not touching it ...it only gets to 22C so I didn't even need the thermostat
and its got shit loads of air around it


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OfflineJuke Adro
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Re: h202 discovery [Re: Juke Adro]
    #8501355 - 06/08/08 08:01 PM (15 years, 11 months ago)

Ok the results are in ......here are some shitty pics but you can still see whats going on

The jars in the TIT with h2o2 are almost 100% colonised



The jars in the TIT with no h2o2 only have growth at the inoculation site 2 inches by 2 inches

the jar by him self is about 70% colonised

so i find that a TIT is not good for incubation but if you add h2o2 it can work better than incubation in a open space ...i will keep on using my TIT with h2o2 from now on


if thats not enough proof that its working do it your self and then talk to me lol

but please try this I'm not good at these experiments and my pics are crap so if you want to try this please do and put a link in this thread so we can all watch it.
This may have been luck and may have been a pure fluke but I doubt it I will try get a better camera tomorrow and take some better pics


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Invisiblewisp

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Re: h202 discovery [Re: Juke Adro]
    #8501397 - 06/08/08 08:11 PM (15 years, 11 months ago)

Nice job man.

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OfflineJuke Adro
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Re: h202 discovery [Re: wisp]
    #8501407 - 06/08/08 08:14 PM (15 years, 11 months ago)

Thanks mate
I'm ashamed of my shitty pics tho :frown:
I will grab a good camera and post better pics tomorrow so all can see it perfectly


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OfflineFirstAvailable
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Re: h202 discovery [Re: Juke Adro]
    #8502340 - 06/09/08 02:07 AM (15 years, 11 months ago)

This is interesting, however, I don't think the product you are using is 50% H2O2.  It might be, but I don't think so.  H2O2 in high concentrations is considered a hazardous material.  The highest concentration that I am aware of that is easy to obtain commercially without special permits is 35%.  Most likely, it is 50% H2O2 solution, with the remaining 50% additives for growth.

Have you tried doing this experiment using just regular H2O2, like the kind you would get at a drug store?  This would be a good idea in order to rule out  the chance that the other "mystery stuff" in your product is the cause of the added growth rate.

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Re: h202 discovery [Re: FirstAvailable]
    #8502377 - 06/09/08 02:39 AM (15 years, 11 months ago)

so you have glasses with foil ontop currect and nothing else?  Well i kind of have a hypothesis then as to why this may be helping growth, its keeping everything alot cleaner


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Re: h202 discovery [Re: makaveli8x8]
    #8502949 - 06/09/08 09:18 AM (15 years, 11 months ago)

We specifically use small gas exchange holes in our jar lids to keep CO2 at a high level during colonization.  You don't want lots of O2.  The reason is to prevent the mycelium from consuming too much of the substrate prior to fruiting.

Increased O2 is a major pinning trigger.  If you colonzize the jars in a high O2 environment, not only does the mycelium consume more of the substrate that you want to save for fruiting, you also remove a major pinning trigger when you move the substrates to your fruiting environment.  It will be interesting to see how these fruit, how many flushes, etc.
RR


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OfflineJuke Adro
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Re: h202 discovery [Re: RogerRabbit]
    #8503875 - 06/09/08 03:05 PM (15 years, 11 months ago)

Im really sorry about my crap pics here are some better ones


above no h2o2


above h2o2


the above pic is the big jar which is even nearly finished which is from the h2o2 TIT :wink:


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OfflineJuke Adro
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Re: h202 discovery [Re: FirstAvailable]
    #8503892 - 06/09/08 03:10 PM (15 years, 11 months ago)

Quote:

FirstAvailable said:
This is interesting, however, I don't think the product you are using is 50% H2O2.  It might be, but I don't think so.  H2O2 in high concentrations is considered a hazardous material.  The highest concentration that I am aware of that is easy to obtain commercially without special permits is 35%.  Most likely, it is 50% H2O2 solution, with the remaining 50% additives for growth.

Have you tried doing this experiment using just regular H2O2, like the kind you would get at a drug store?  This would be a good idea in order to rule out  the chance that the other "mystery stuff" in your product is the cause of the added growth rate.





Yes it is 50% h2o2 no additives and yes it is very dangerous.
I have had multiple burns with this product and had a few other issues with it .....I have a good friend at the hydro shop where I buy my equipment 

No I have not used regular h2o2 I only use 50%

I'm also not willing to do anymore testing of it i will just spawn and show my pics so you all can see it does not affect growth


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OfflineJuke Adro
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Re: h202 discovery [Re: RogerRabbit]
    #8503917 - 06/09/08 03:18 PM (15 years, 11 months ago)

Quote:

RogerRabbit said:
We specifically use small gas exchange holes in our jar lids to keep CO2 at a high level during colonization.  You don't want lots of O2.  The reason is to prevent the mycelium from consuming too much of the substrate prior to fruiting.

Increased O2 is a major pinning trigger.  If you colonzize the jars in a high O2 environment, not only does the mycelium consume more of the substrate that you want to save for fruiting, you also remove a major pinning trigger when you move the substrates to your fruiting environment.  It will be interesting to see how these fruit, how many flushes, etc.
RR




I will record everything and take pictures on the way from spawning which will be done asap, i just have to pasteurise my horse poo, I will only use h2o2 test jars to spawn, that poo which I spawn too will also go in the h2o2 TIT

so please keep this thread open till my last picture of my last flush is finished :smile:
and then hopefully someone else can give it a shot, and give us there feed back


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Edited by Juke Adro (06/09/08 05:03 PM)

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Invisiblewisp

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Re: h202 discovery [Re: Juke Adro]
    #8504454 - 06/09/08 05:39 PM (15 years, 11 months ago)

Nice new pics man. God strong growth. It's been very interesting following this. It's hard to argue with results. Strange coloured myc there though.

By the way, where do you get 50% H2O2 from?

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OfflineJuke Adro
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Re: h202 discovery [Re: wisp]
    #8504499 - 06/09/08 05:51 PM (15 years, 11 months ago)

I get 50% from a mate he owns a shop down here...I asked him if it was really 50%  a while back when someone else doubted that i had 50%, my mate told me to tell them to get stuffed lol and assured me that it is. also any test is welcome on it .....

and trip if you will do this experiment I will send you one brand new bottle of oxyplus h2o2 50%.


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