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OfflineJuke Adro
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Re: h202 discovery [Re: legallyhomeless]
    #8424718 - 05/20/08 04:35 PM (16 years, 2 days ago)

Quote:

legallyhomeless said:
I think that if you do try this again and document it, you will need to try 1 tub w/ oxy, 1 tub w/o oxy. 1 incubator w/ oxy, 1 incubator w/o oxy. even though it will be multispore it will still be better then only having 1 thing to compare to, it will just bring more negativity




ok il do that and see what happens il start asap and see where it takes us


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OfflineJuke Adro
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Re: h202 discovery [Re: MrCubensis]
    #8424727 - 05/20/08 04:37 PM (16 years, 2 days ago)

Quote:

MrCubensis said:
pics of anything?




i only have a camera on my phone 2.5 mega pixel they wont be the best but il start uploading and documenting friday as im free then :smile:


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Invisiblewisp

Registered: 04/13/08
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Re: h202 discovery [Re: Juke Adro]
    #8424800 - 05/20/08 04:53 PM (16 years, 2 days ago)

:thumbup:

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Offlinesephiroth3535
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Re: h202 discovery [Re: wisp]
    #8435894 - 05/23/08 05:18 AM (15 years, 11 months ago)

I just recently read this...I have some fruiting bodies going on, so I was like what the hell! I took a water bottle, filled it like 3/4 way with peroxide, and then threw some old silver dimes into the mix. WOW! Within MINUTES the coin starts bubbling like insane. Pure oxygen. Its been in there for a few hours now and is still bubbling, I don't see how this can not be good for the mushrooms! You can literally see the reaction and the oxygen going into the air, so I don't know how its even arguable that this doesn't release oxygen into your FC.

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OfflineJuke Adro
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Re: h202 discovery [Re: sephiroth3535]
    #8435896 - 05/23/08 05:20 AM (15 years, 11 months ago)

in the incubator not the fc most of us put it in the fc but this thread is about it being in the incubator :wink:


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OfflineDigital Reality
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Re: h202 discovery [Re: Juke Adro]
    #8436301 - 05/23/08 09:40 AM (15 years, 11 months ago)

Sorry to be off topic but god damn I hate your avatar so much. I can’t think about anything other than those fucking tits flopping around everywhere. lol that girl is so damn hot. It seems to be a love hate thing. Im like mesmerized by her but god damn I hate it cuz I can’t concentrate for more than 3 seconds on what im reading. lol

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Offlinexhooliganx
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Re: h202 discovery [Re: WeBeBad]
    #8436478 - 05/23/08 10:55 AM (15 years, 11 months ago)

Quote:

WeBeBad said:
Quote:

Juke Adro said:
its not broken fully down it's humidifying the incubator so its still hydrogen peroxide not oxygen!!!!!!!!!!!! it would be dry if it was broken down completely (I think lol) and its not damaging it cause its fine as you would notice if you read what i wrote previously.




I think you've stepped out of your area of expertise to be making assumptions like these. You, yourself, already said you used a dilute solution of hydrogen peroxide. Do you know what's in a solution of hydrogen peroxide? Water and H2O2, but you should know that. Whatever observable increased humidity levels being caused are coming from WATER vapor. Hydrogen peroxide breaks down into hydrogen and oxygen gas. Please don't make silly assumptions and please don't step out of your area of expertise. It'll only serve to confuse people.



I thought h2o2 breaks down into h2o and oxygen gas. that was my favorite part about this whole topic was that the OP is stuck on there being some kind of h2o2 humidity vapor stimulating growth in his incubator. when any kind of humidity would be h2o and there would be a slight increase in oxygen. RR already explained that increasing oxygen isn't optimal and if you wanted higher gas exchange with more oxygen you could just put a filter disc under the band with no restriction.

as for adding a scuba tank it would be more cost effective to just add a fan because a scuba tank isn't filled with oxygen it's filled with air. the same stuff that a fan would blow into a incubator.

to everyone bitching about people being negative or "brainy know it alls" if you can't handle sceptacism, logic, and science then maybe the advanced forum isn't the place to talk about experimental unproven methods.


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OfflineJuke Adro
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Re: h202 discovery [Re: xhooliganx]
    #8442846 - 05/25/08 06:03 AM (15 years, 11 months ago)

Quote:

xhooliganx said:
Quote:

WeBeBad said:
Quote:

Juke Adro said:
its not broken fully down it's humidifying the incubator so its still hydrogen peroxide not oxygen!!!!!!!!!!!! it would be dry if it was broken down completely (I think lol) and its not damaging it cause its fine as you would notice if you read what i wrote previously.




I think you've stepped out of your area of expertise to be making assumptions like these. You, yourself, already said you used a dilute solution of hydrogen peroxide. Do you know what's in a solution of hydrogen peroxide? Water and H2O2, but you should know that. Whatever observable increased humidity levels being caused are coming from WATER vapor. Hydrogen peroxide breaks down into hydrogen and oxygen gas. Please don't make silly assumptions and please don't step out of your area of expertise. It'll only serve to confuse people.



I thought h2o2 breaks down into h2o and oxygen gas. that was my favorite part about this whole topic was that the OP is stuck on there being some kind of h2o2 humidity vapor stimulating growth in his incubator. when any kind of humidity would be h2o and there would be a slight increase in oxygen. RR already explained that increasing oxygen isn't optimal and if you wanted higher gas exchange with more oxygen you could just put a filter disc under the band with no restriction.

as for adding a scuba tank it would be more cost effective to just add a fan because a scuba tank isn't filled with oxygen it's filled with air. the same stuff that a fan would blow into a incubator.

to everyone bitching about people being negative or "brainy know it alls" if you can't handle sceptacism, logic, and science then maybe the advanced forum isn't the place to talk about experimental unproven methods.




i completely see what your saying but.....if it broke down into h20 it would not burn my hands by touching the lid of the incubator......so what your hypothisis is....is just a hypothisis and just because RR said something it dont mean you have to take his advice, he is not god and il admit he knows his shit, but it dont mean it wont work in fact it does work ....it speeds up growth weather you like it or not and it does get in the jars and will burn the mycelium try it and see

it might eat up all your nutes and it might dry out your jars/casings and it might initiate fruiting ....but it makes the mycelium grow faster by at least 25% so unless you are going to do it and see for your self i dont want your hypothisis or smart arse remarks

and if i choose to put it in advanced mycology thats where it will be.

Adding h2o2 in the incubator makes mycelium grow faster weather its good for it or not thats my fucking point now lets see you come up with anything that works, all i am trying to do is show what i found not make a argument


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OfflineSeussA
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Re: h202 discovery [Re: Juke Adro]
    #8442892 - 05/25/08 06:35 AM (15 years, 11 months ago)

> and if i choose to put it in advanced mycology thats where it will be.

And if I choose to move it to the dump, that is where it will remain. Enough with the attitude. That goes for everybody.

> but it makes the mycelium grow faster by at least 25%

Have you performed a controlled experiment? Has it been repeated by others? If not, then you are guessing. (A controlled experiment would compare growth with and without the h2o2, holding everything else equal, in several different environments, to ensure that it is the h2o2 and not something else that is causing the increase in growth.)


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OfflineJuke Adro
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Re: h202 discovery [Re: Seuss]
    #8442899 - 05/25/08 06:39 AM (15 years, 11 months ago)

Yeah im doing at as we speak.

I have asked other to do it also, I think tripsis said he is going to do it in pm


Sorry about my attitude if I was rude I'm sorry, I just cant handle people picking without trying it, my bad.


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OfflineRogerRabbitM
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Re: h202 discovery [Re: Juke Adro]
    #8444072 - 05/25/08 04:37 PM (15 years, 11 months ago)

If you think this is bad, try going to a scientific research site. They're rabid, but if it passes muster with them, you then have something to take to the bank.

As Seuss said, can the attitudes. I almost dumped this thread last week, but decided to let it run its course.

Perhaps the O2 is helping to make up for the lost rate of growth due to having your jars in a silly incubator instead of where they can have air circulating around them like you're supposed to do? If you have jars stuck down in a tub, there's no doubt they're sitting in stale air and O2 would help offset that somewhat. However, IF that's the case, the same increase in growth(or loss of the decrease) would be achieved by placing the jars on an open shelf. Remember, many controlled experiments have shown virtually no increase in rate of growth from 75F to 82F, and above 82F, growth begins to fall off. If you have 'normal room temperature' which is mid 70's you don't need that box anyway.
RR


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Offlineozgrove
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Re: h202 discovery [Re: RogerRabbit]
    #8445292 - 05/25/08 11:23 PM (15 years, 11 months ago)

Hey people, Wsup ? I don`t know why peroxide on Incubating chamber would be a detriment. This is some GOOD SHIT!!
Just clik this!Then clik on FAQ on Peroxide. Read this , and make up your mind, whether you like H2O2 or not!!

Peroxide

Later, OZGROVE

Edited by ozgrove (05/25/08 11:26 PM)

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OfflineJuke Adro
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Re: h202 discovery [Re: RogerRabbit]
    #8446087 - 05/26/08 07:07 AM (15 years, 11 months ago)

Quote:

RogerRabbit said:
If you think this is bad, try going to a scientific research site. They're rabid, but if it passes muster with them, you then have something to take to the bank.

As Seuss said, can the attitudes. I almost dumped this thread last week, but decided to let it run its course.

Perhaps the O2 is helping to make up for the lost rate of growth due to having your jars in a silly incubator instead of where they can have air circulating around them like you're supposed to do? If you have jars stuck down in a tub, there's no doubt they're sitting in stale air and O2 would help offset that somewhat. However, IF that's the case, the same increase in growth(or loss of the decrease) would be achieved by placing the jars on an open shelf. Remember, many controlled experiments have shown virtually no increase in rate of growth from 75F to 82F, and above 82F, growth begins to fall off. If you have 'normal room temperature' which is mid 70's you don't need that box anyway.
RR





yeah you may be right, thats why I'm testing it so I should be able to give some really good answers in 10 days.

2 jars in a open room on a shelf, 2 jars in a tub in tub with 50mls h2o2 held at 75f and 2 and another tub in tub with no h2o2 held at 75f

God, you guys are really set in your ways yeah.


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Invisiblewisp

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Re: h202 discovery [Re: Juke Adro]
    #8446090 - 05/26/08 07:10 AM (15 years, 11 months ago)

It the scientific approach. It needs to be rigourous. But it'll be all the better if you get positive results.

Edited by wisp (05/26/08 07:15 AM)

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InvisibleJaComet
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Re: h202 discovery [Re: ozgrove]
    #8446102 - 05/26/08 07:26 AM (15 years, 11 months ago)

H2O2 based oxygen products intended for agricultural use are stabilized with additives in order to control natural reactivity of the product, which increases with temperature.

H2O2 does give off reactive vapor which, in higher concentrations, will cause notable sanitizing / bleaching.

H2O2 can be carried by water vapor at any concentration level.


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OfflineJuke Adro
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Re: h202 discovery [Re: wisp]
    #8446141 - 05/26/08 07:57 AM (15 years, 11 months ago)

Quote:

tripsis said:
It the scientific approach. It needs to be rigourous. But it'll be all the better if you get positive results.




I better get positive results otherwise these guys in here will say ....I told ya so, and il look like a fool but either way I'm happy to provide a result weather it is good or bad.


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OfflineJuke Adro
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Re: h202 discovery [Re: JaComet]
    #8446150 - 05/26/08 08:05 AM (15 years, 11 months ago)

Quote:

JaComet said:
H2O2 based oxygen products intended for agricultural use are stabilized with additives in order to control natural reactivity of the product, which increases with temperature.

H2O2 does give off reactive vapor which, in higher concentrations, will cause notable sanitizing / bleaching.





H2O2 can be carried by water vapor at any concentration level.





Thank you, that explains why my knuckles turned white and burned when they touched the inside of the lid.

So now it would also explain why the mycelium turns blue on the surface of the substrate

so we are getting somewhere.


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InvisibleJaComet
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Re: h202 discovery [Re: Juke Adro]
    #8454063 - 05/28/08 06:11 AM (15 years, 11 months ago)

You're welcome JA.

Be certain to treat this stuff with due respect and caution. 50% is a very high concentration. I note the current Oxy-Plus product is listed at 35%, which is the concentration used in aerobic sterilization of food packaging.

Keep liquid and vapors away from eyes and mucus membranes.

It degrades most plastics.

If opting to use Food Grade, store in freezer.

Good luck with your trials.


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OfflineJuke Adro
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Re: h202 discovery [Re: JaComet]
    #8475586 - 06/02/08 04:29 PM (15 years, 11 months ago)

ok I know It has taken some time but I'm too busy with work,

ok so normal drinking glasses were observed to have had the boosted growth so its drinking glasses I will use and brown rice was the substrate so brown rice will be used the only thing im not 100 percent sure of was how much h2o2 to the mls so i will use 50ml of 50% h202

5 cups were inoculated today at 6am with multi spore syringes all of the same strain 2cc were used per cup 10cc all up

1 cup sit's on the shelf in a room at temps between 14C and 20C

2 cups sit in a TIT at a constant 23C with 50ml h202 chamber is 80ltrs

2 cups will sit in a TIT at a constant 23C with no h2o2 chamber is 80ltrs

I'm so confident it works that I put one large jar not sure of the size and a larger cup in the h2o2 TIT cause Im sure they will be fully colonised before the other non h2o2 ones

anyway one more note I might have to add more h2o2 in the TIT if it runs out but i will let you know if i do and how much .....this is my first test ever besides school so if you want to help please tell me how to make it better..






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Invisiblewisp

Registered: 04/13/08
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Re: h202 discovery [Re: Juke Adro]
    #8476143 - 06/02/08 06:35 PM (15 years, 11 months ago)

Interesting man. I can't help with the experiment anymore, I deconstructed my TiT. But this is certainly something to keep in mind.

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