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Invisiblewisp

Registered: 04/13/08
Posts: 5,304
Re: h202 discovery [Re: Juke Adro]
    #8414397 - 05/17/08 08:37 PM (16 years, 5 days ago)

h2o2 still has the same amount of oxygen as h20, just an extra hydrogen atom, so i can't see it being any different to humidifying with water.

Quote:

RogerRabbit said:
It actually sounds more like a coincidence. However, increased oxygen during colonization would not necessarily be a good thing. We use small gas exchange holes in our lids precisely to keep the CO2 levels high during colonization. Doing so prevents the mycelium from consuming too much of the substrate during this period. Increasing the O2 content of the air is one of the main pinning triggers, and during the jar colonization stage, you don't want to introduce pinning triggers.
RR




keeping this in mind it's probable that the growth changed because of the increased oxygen levels, but it still isn't necessarily something desirable. as oxygen is a pinning trigger, if you expose your mycelium to oxygen during incubation, come fruiting it might be problematic getting it to fruit.

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OfflineJuke Adro
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Re: h202 discovery [Re: wisp]
    #8414453 - 05/17/08 08:47 PM (16 years, 5 days ago)

yeah not sure trip, just be nice if someone else tried it so i know weather it was a coincidence or in fact quicker growth.

im skeptical too but i tried it again and same thing happened sooo i dunno 2 coincidences in a row ?


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Invisiblewisp

Registered: 04/13/08
Posts: 5,304
Re: h202 discovery [Re: Juke Adro]
    #8414481 - 05/17/08 08:53 PM (16 years, 5 days ago)

did you notice any early pinning? maybe i'll give this a go to see what happens

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OfflineJuke Adro
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Re: h202 discovery [Re: wisp]
    #8414512 - 05/17/08 09:01 PM (16 years, 5 days ago)

well the casing pinned very quick after i took it out but the grain did not pin and looked perfect now its in shit


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Invisiblewisp

Registered: 04/13/08
Posts: 5,304
Re: h202 discovery [Re: Juke Adro]
    #8414550 - 05/17/08 09:11 PM (16 years, 5 days ago)

how's it in shit?

putting h202 in my incubator would probably be a good thing as i just found 2 glasses with Neurospora (pink mould) in them, a particularly pernicious contam. fuck!

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OfflineJuke Adro
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Re: h202 discovery [Re: wisp]
    #8414685 - 05/17/08 09:45 PM (16 years, 5 days ago)

i spawned some to horse poo is what i meant lol


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OfflineTsukishade
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Re: h202 discovery [Re: wisp]
    #8415641 - 05/18/08 07:10 AM (16 years, 4 days ago)

Quote:

tripsis said:
h2o2 still has the same amount of oxygen as h20, just an extra hydrogen atom, so i can't see it being any different to humidifying with water.




Actually, it contains another oxygen atom. H2O2 is a very strong oxidizer. It decomposes spontaneously into oxygen and water in the following exothermic reaction:

2 H2O2 → 2 H2O + O2

Also, Juke, your product is 50% peroxide solution or 50 Volume? The difference is as follows: 1% peroxide releases 3.3 volumes of oxygen during decomposition. A 10 Vol. solution is at 3% concentration, a 50 Vol. is at 15%.


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Invisiblewisp

Registered: 04/13/08
Posts: 5,304
Re: h202 discovery [Re: Tsukishade]
    #8415654 - 05/18/08 07:24 AM (16 years, 4 days ago)

Oh shit yeah, what was I thinking? I blame being sick! Lol. Fuck that was daft.

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OfflineWeBeBad
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Registered: 05/08/07
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Re: h202 discovery [Re: Juke Adro]
    #8416106 - 05/18/08 12:10 PM (16 years, 4 days ago)

Quote:

Juke Adro said:
its not broken fully down it's humidifying the incubator so its still hydrogen peroxide not oxygen!!!!!!!!!!!! it would be dry if it was broken down completely (I think lol) and its not damaging it cause its fine as you would notice if you read what i wrote previously.




I think you've stepped out of your area of expertise to be making assumptions like these. You, yourself, already said you used a dilute solution of hydrogen peroxide. Do you know what's in a solution of hydrogen peroxide? Water and H2O2, but you should know that. Whatever observable increased humidity levels being caused are coming from WATER vapor. Hydrogen peroxide breaks down into hydrogen and oxygen gas. Please don't make silly assumptions and please don't step out of your area of expertise. It'll only serve to confuse people.

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Offlinepoof
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Registered: 08/07/07
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Re: h202 discovery [Re: Juke Adro]
    #8417164 - 05/18/08 04:45 PM (16 years, 4 days ago)

ok you found something great that works for you, whats your point in trying to convince these know-it-all losers that it works? hell, if i were you i would have kept it to myself just so others could fail harder than they already do.

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Invisiblelegallyhomeless
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Registered: 07/01/05
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Re: h202 discovery [Re: poof]
    #8417961 - 05/18/08 08:15 PM (16 years, 4 days ago)

This forum is about learning not hiding information.


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Invisiblewisp

Registered: 04/13/08
Posts: 5,304
Re: h202 discovery [Re: legallyhomeless]
    #8419285 - 05/19/08 06:55 AM (16 years, 3 days ago)

agreed, it would never progress if everyone hid everything they learned and knew.

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InvisibleJohn NadaDiscord
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Re: h202 discovery [Re: wisp]
    #8419560 - 05/19/08 10:03 AM (16 years, 3 days ago)

Ha, this should be moved to the regular cultivation forum. I just realized this was in the Advanced forum.

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OfflineRogerRabbitM
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Re: h202 discovery [Re: John Nada]
    #8420250 - 05/19/08 02:24 PM (16 years, 3 days ago)

I'll leave it here for now, but if the thread continues to degrade with no additional data, I'll just lock it.

Personally, I think the effects of adding O2 to colonizing jars are deleterious, even if it does speed up colonization. You don't want your substrate being consumed prior to the fruiting stage, so that the nutrients in the substrate are available for the fruitbodies. That's the reason we use very small holes for gas exchange rather than simply placing a filter over the top of the jar. It's important to keep the CO2 levels high during colonization.
RR


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OfflineJuke Adro
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Re: h202 discovery [Re: Tsukishade]
    #8420449 - 05/19/08 03:20 PM (16 years, 3 days ago)

Quote:

Tsukishade said:
Quote:

tripsis said:
h2o2 still has the same amount of oxygen as h20, just an extra hydrogen atom, so i can't see it being any different to humidifying with water.




Actually, it contains another oxygen atom. H2O2 is a very strong oxidizer. It decomposes spontaneously into oxygen and water in the following exothermic reaction:

2 H2O2 → 2 H2O + O2

Also, Juke, your product is 50% peroxide solution or 50 Volume? The difference is as follows: 1% peroxide releases 3.3 volumes of oxygen during decomposition. A 10 Vol. solution is at 3% concentration, a 50 Vol. is at 15%.




it says on the bottle 50% active ingredient hydrogen peroxide the other 50% would be just water imo but i can not be 100% cause all it says is 50% hydrogen peroxide here is the bottle

i dont dilute it just use it straight out of the bottle


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Edited by Juke Adro (05/19/08 03:30 PM)

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OfflineJuke Adro
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Re: h202 discovery [Re: WeBeBad]
    #8420486 - 05/19/08 03:28 PM (16 years, 3 days ago)

Quote:

WeBeBad said:
Quote:

Juke Adro said:
its not broken fully down it's humidifying the incubator so its still hydrogen peroxide not oxygen!!!!!!!!!!!! it would be dry if it was broken down completely (I think lol) and its not damaging it cause its fine as you would notice if you read what i wrote previously.




I think you've stepped out of your area of expertise to be making assumptions like these.  You, yourself, already said you used a dilute solution of hydrogen peroxide.  Do you know what's in a solution of hydrogen peroxide?  Water and H2O2, but you should know that.  Whatever observable increased humidity levels being caused are coming from WATER vapor.  Hydrogen peroxide breaks down into hydrogen and oxygen gas.  Please don't make silly assumptions and please don't step out of your area of expertise.  It'll only serve to confuse people.




look mate i understand what your saying ...but
i am just telling what i observed so either you can try it and see for your self or simply just ignore it,
it wont confuse anyone im not a braniac but i saw a big difference and thought i would share it, weather you like it or not.
I dont want negativity i just wanted to share what happened and maybe for someone to confirm or deny

and lastly i will be adding h202 in my fc till the day i die :wink:


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OfflineJuke Adro
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Re: h202 discovery [Re: Juke Adro]
    #8420522 - 05/19/08 03:36 PM (16 years, 3 days ago)

I'm going to test it one more time, 3 all up this time i will start from ms, inoculate, spwan to poo incubate, case incubate and fruit i will document everything with times pictures the works


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OfflineJuke Adro
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Re: h202 discovery [Re: legallyhomeless]
    #8420530 - 05/19/08 03:38 PM (16 years, 3 days ago)

Quote:

legallyhomeless said:
This forum is about learning not hiding information.




:thumbup: ture even if its not practical at least we will know "not to add h202 to a fc" either way you find out something


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Invisiblelegallyhomeless
mooch
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Registered: 07/01/05
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Re: h202 discovery [Re: Juke Adro]
    #8423302 - 05/20/08 07:17 AM (16 years, 2 days ago)

I think that if you do try this again and document it, you will need to try 1 tub w/ oxy, 1 tub w/o oxy. 1 incubator w/ oxy, 1 incubator w/o oxy. even though it will be multispore it will still be better then only having 1 thing to compare to, it will just bring more negativity


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OfflineMrCubensis
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Re: h202 discovery [Re: Juke Adro]
    #8424002 - 05/20/08 12:19 PM (16 years, 2 days ago)

pics of anything?


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