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OfflineJuke Adro
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h202 discovery (NEW HIGH RES PICS UPDATED) (NEW UPDATE 17-6-08)
    #8410381 - 05/16/08 05:46 PM (16 years, 6 days ago)

i was cleaning my incubator and spilled some h202 in there and forgot to wipe it up, i put all my gear back in then 2 days later i checked my incubator to see how things were going as im impatient, my fingers started to burn slightly and went white as they do when one touches h202 and that was when i realized what had happened .....my jars and tubs looked all 100% colonized but had a blue tinge to them all on the surface and the h202 was being evaporated and made the whole chamber humid.

now the funny part 2 days before i had the spill things were only 50% colonized and 2 days later it was all 90% to 100% which made me believe it's something worth posting  what i think happened that it added oxygen to the chamber and the growth just took off and grew so fast i shit my self, it is not a contam as i have some pinning already, and i believe the blue tinge to the top of the myc is just where it got touched by h202


i tested this again to see what would happen i made up a new incubator put 4 50% colonized jars and 3 fresh casings in the chamber added 50mls of h202 and incubated at 25c for 3 days agian the myc took off like never before and the mycelium at the top of the substrate was slightly blue and the mycelium becomes very  ropey and thick completely different to any rizo myc or cottoned  myc it takes on a whole new look which i find fascinating

anyway can some other people test this and let me know if i stumbled across something good or just some thoughts on what happened.



above no h2o2


above h2o2


the above pic is the big jar which is even nearly finished which is from the h2o2 TIT :wink:


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Edited by Juke Adro (06/16/08 03:19 PM)

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Invisiblelegallyhomeless
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Re: h202 discovery [Re: Juke Adro]
    #8410474 - 05/16/08 06:16 PM (16 years, 6 days ago)

This sounds really interesting. How did the h202 touch the mycelium in the jars? Im curious how they turned blue.


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OfflineJuke Adro
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Re: h202 discovery [Re: legallyhomeless]
    #8410542 - 05/16/08 06:33 PM (16 years, 6 days ago)

it did not really touch them it was just a figure of speach more like i believe the humidity of the h202 breathed through the holes and foil, it was only very slight bluing,
and you can see where the myc climbed the walls trying to get out of the holes very very interesting

if you can imagine a fc full of humidity with h20, but this way its the incubator and h202 humidity but only a small amount of it.
i really think that it's something here the mycelium really changes from thin riz growth to very think riz growth and not cottony it also climbs the walls trying to get out and builds up near the holes to get out, but those areas are more blue and its not damaged cause it still grows fast as hell and fruits (well pinning atm)


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Edited by Juke Adro (05/16/08 06:40 PM)

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OfflinexFrockx
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Re: h202 discovery [Re: Juke Adro]
    #8410865 - 05/16/08 08:27 PM (16 years, 6 days ago)

It would seem to make sense that the excess 02 released by the peroxide would help growth, I think this could be one of the more promising things I've seen on here in a while when it comes to simplicity.

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Re: h202 discovery [Re: xFrockx]
    #8411068 - 05/16/08 09:35 PM (16 years, 6 days ago)

This is exactly one of the reasons that I put lots of H2O2 in the perlite of my fruiting chambers. Not only does it prevent it from getting funky, but I've always suspected that it boosts the oxygen levels in the chamber slightly. It certainly can't hurt.

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InvisibleJohn NadaDiscord
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Re: h202 discovery [Re: Juke Adro]
    #8411543 - 05/17/08 12:15 AM (16 years, 5 days ago)

Watch out for h202 especially if it's undiluted too much will delay or even kill mycelium.

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Invisibleflavoraid
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Re: h202 discovery [Re: John Nada]
    #8412129 - 05/17/08 07:47 AM (16 years, 5 days ago)

Quote:

paradis said:
Watch out for h202 especially if it's undiluted too much will delay or even kill mycelium.




it wasn't directly applied.


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coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.

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InvisibleJohn NadaDiscord
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Re: h202 discovery [Re: flavoraid]
    #8412521 - 05/17/08 11:34 AM (16 years, 5 days ago)

Oh I see. Sorry, well that shouldn't hurt then

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Invisibleflavoraid
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Re: h202 discovery [Re: John Nada]
    #8412633 - 05/17/08 12:19 PM (16 years, 5 days ago)

I'll have to try this, hell I might try attaching my scuba gear to my incubator even.

IMO.. i dont think it would make that much difference because people would have realised this by now and would use larger gas exchange on there covers for casigns while incubating.

fahster has experimented with his oven bag grows and he was change a fair bit of oxygen while his substrate colonized I think multiple changes a day, didn't report anything about increased colonization rate.


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coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.

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Re: h202 discovery [Re: flavoraid]
    #8412969 - 05/17/08 01:54 PM (16 years, 5 days ago)

You could also just use a fan with a filter over the intake.

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Re: h202 discovery [Re: John Nada]
    #8413003 - 05/17/08 02:03 PM (16 years, 5 days ago)

thinking of this... I actually don't think it's possible to have great increase in growth.

People have like open room incubators with virtually limitless amounts of oxygen..... that's my point :P


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coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.

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OfflineJuke Adro
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Re: h202 discovery [Re: flavoraid]
    #8413372 - 05/17/08 03:24 PM (16 years, 5 days ago)

Quote:

flavoraid said:
thinking of this... I actually don't think it's possible to have great increase in growth.

People have like open room incubators with virtually limitless amounts of oxygen..... that's my point :P




yeah but this is different, its not normal oxygen its making the incubator humid with h202 oxygen .....not normal stale dry oxygen, please read properly before you make assumptions


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Edited by Juke Adro (05/17/08 03:28 PM)

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OfflineJuke Adro
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Re: h202 discovery [Re: flavoraid]
    #8413384 - 05/17/08 03:26 PM (16 years, 5 days ago)

Quote:

flavoraid said:
I'll have to try this, hell I might try attaching my scuba gear to my incubator even.

IMO.. i dont think it would make that much difference because people would have realised this by now and would use larger gas exchange on there covers for casigns while incubating.

fahster has experimented with his oven bag grows and he was change a fair bit of oxygen while his substrate colonized I think multiple changes a day, didn't report anything about increased colonization rate.




I know what your saying but try it before you make a assumption, it really makes a big difference the stuff i use is called .....oxy plus active ingredient 50% hydrogen peroxide, im guessing the other 50% is water it does not say on the bottle what the other 50% is.

anyway you will see how the myc tries to climb out of your jars its really cool


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OfflineGrogan
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Re: h202 discovery [Re: Juke Adro]
    #8413965 - 05/17/08 06:46 PM (16 years, 5 days ago)

What percentage concentration was it thats pretty damn important to know if we wanted to try this out!! I will give it a go for sure some day soon.


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OfflineRogerRabbitM
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Re: h202 discovery [Re: Juke Adro]
    #8413968 - 05/17/08 06:47 PM (16 years, 5 days ago)

Many growers have placed peroxide in perlite and humidifiers, but with no increased growth. To test peroxide, I once brought home an air analyzer from work and tested the O2 content of the air, then added 35% industrial grade peroxide to the humidifier(no water). There was no noticeable increase in O2 levels in the fruiting chamber.

It actually sounds more like a coincidence. However, increased oxygen during colonization would not necessarily be a good thing. We use small gas exchange holes in our lids precisely to keep the CO2 levels high during colonization. Doing so prevents the mycelium from consuming too much of the substrate during this period. Increasing the O2 content of the air is one of the main pinning triggers, and during the jar colonization stage, you don't want to introduce pinning triggers.
RR


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OfflineJuke Adro
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Re: h202 discovery [Re: Grogan]
    #8413973 - 05/17/08 06:49 PM (16 years, 5 days ago)

50% hydrogen peroxide the actual product is from growtek and called oxy plus


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OfflineJuke Adro
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Re: h202 discovery [Re: Juke Adro]
    #8413985 - 05/17/08 06:53 PM (16 years, 5 days ago)

RR I'm talking about in the incubator....not the fc.
It could very well be a coincidence but why would the mycelium change its appearance and start trying to grow out of the jars with slight bluing on the top and more blue where it tries to climb up


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OfflineJuke Adro
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Re: h202 discovery [Re: Juke Adro]
    #8414017 - 05/17/08 07:02 PM (16 years, 5 days ago)

another observation, the substrate seems to dry out faster than normal.

I understand about the pinning but i saw no problems as yet, but in jars of grain I would not think it would but who knows? I saw no pinning until it went in the fc, when re incubating casings with a fresh layer, its what you would want is it not?

but i can only wait till someone else tries it to confirm or close the case


also RR i know you know your shit so maybe you could add some to your incubator and observe it for 3 days and get back with your findings ?


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Edited by Juke Adro (05/17/08 07:07 PM)

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Invisibleflavoraid
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Re: h202 discovery [Re: Juke Adro]
    #8414166 - 05/17/08 07:47 PM (16 years, 5 days ago)

Quote:

Juke Adro said:
Quote:

flavoraid said:
thinking of this... I actually don't think it's possible to have great increase in growth.

People have like open room incubators with virtually limitless amounts of oxygen..... that's my point :P




yeah but this is different, its not normal oxygen its making the incubator humid with h202 oxygen .....not normal stale dry oxygen, please read properly before you make assumptions




uh so you'de have two parts - h202 which damages mycelium and oxygen.... all h202 does when broken down is release oxygen


--------------------
coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.

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OfflineJuke Adro
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Re: h202 discovery [Re: flavoraid]
    #8414207 - 05/17/08 07:57 PM (16 years, 5 days ago)

its not broken fully down it's humidifying the incubator so its still hydrogen peroxide not oxygen!!!!!!!!!!!! it would be dry if it was broken down completely (I think lol) and its not damaging it cause its fine as you would notice if you read what i wrote previously.

just because the mycelium is slightly blue does not mean its fucked my guess was it grew too fast which caused that to happen and got slightly too much h202


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