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Offlinepothead_bob
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Is this mycelium started from liquid culture done yet?
    #8362897 - 05/04/08 08:53 PM (16 years, 8 months ago)

I started 9 1-pint jars using liquid culture 17 days ago. The strain is P. Cubensis Ecuador. I injected 10 ccs of LC into each jar (i know, its a lot), but I made the substrate extra dry to make up for the extra liquid. The mycelium grew differently than Im used to seeing when starting from spore - the substrate started turning whiter each day instead of little white spots forming and growing. Because the entire cake keeps turning whiter, how will I know when full colonization has taken place?

Over the past 5 days or so, I noticed that the bottom of the jars looks whiter and more like my last set of colonized cakes when compared to the upper part of the jar. Ive also been seeing a very slight purple or pink hue to the mycelium on the upper part of the cake, but its really hard to tell. I keep doubting myself when I look at it, so Im not sure if its contamination or just the way the light is hitting the jars.

I added some pics, but theyre kind of blurry. The first two pics were taken with flash, so they look whiter than they are. The second two pics were taken without the flash.









You think these are close to being done and ready for birthing?


--------------------
No knowledge can be certain, if it is not based
upon mathematics or upon some other knowledge
which is itself based upon the mathematical
sciences.
  -Leonardo da Vinci (1425-1519)

Speak well of your enemies.  After all, you made them.

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Offlinetpihkal
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Re: Is this mycelium started from liquid culture done yet? [Re: pothead_bob]
    #8362956 - 05/04/08 09:08 PM (16 years, 8 months ago)

I'd say give them a bit longer, maybe another week or so.


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Offlinepazzy
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Re: Is this mycelium started from liquid culture done yet? [Re: tpihkal]
    #8362966 - 05/04/08 09:10 PM (16 years, 8 months ago)

As soon as you can not see grains they are done.


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OfflineMad River
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Re: Is this mycelium started from liquid culture done yet? [Re: tpihkal]
    #8363166 - 05/04/08 09:54 PM (16 years, 8 months ago)

Why did you use 10cc's per jar?

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OfflineFahkface
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Re: Is this mycelium started from liquid culture done yet? [Re: Mad River]
    #8363288 - 05/04/08 10:29 PM (16 years, 8 months ago)

Quote:

As soon as you can not see grains they are done.




I guess then they would have been done, the very moment you injected, the LC, since it looks like he used verm/brf, not grains.

Beside the fact, that you should have used half pint jars instead of pint jars, making  "dry" cakes, to knock them up with 10cc of LC, isn't very clever.
First of all, you're very likely to fuck up the overall water content of your jars.
Furthermore you're not really able to distribute the LC (water) in your jars, evenly, which -together with using pint jars-, often results in water pools on the bottoms of the jars, while the top stays too dry.
This often causes a bacterial or mold infection, on the bottom and an absence of mycelium on the top.

Since your pics are not very good, I can't really tell, if the mycelium is OK, but it looks kind of weird, especially in the last pic.
The structure of the "mycelium" looks a little unusual, since it seems to starts growing from too many points.
Often mold infected substrate starts off like this.
Anyway, I wouldn't want to say it actually is mold, because I really can't tell from the pics.

However, anything purple/pink is indeed VERY bad, since this would be Neurospora, which recreates extremely fast.

Watch it carefully! As soon as you can confirm it's coloured, remove it! You wouldn't want this shit to infest your growing facilities, since it's almost impossible to remove it later!

Mh, sounds like a very negative response hu?

Isn't meant to...

good luck :thumbup:

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Offlinepothead_bob
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Re: Is this mycelium started from liquid culture done yet? [Re: Fahkface]
    #8364187 - 05/05/08 07:42 AM (16 years, 8 months ago)

When I did my last grow with 1/2 pint jars they took forever to colonize; they started fast and then about halfway down, they colonization slowed. Since I was using 1 pint jars this time (didn't have any 1/2 pint jars at the time and read that others used pint jars successfully) I wanted to make sure the colonization didn't take so long, so I used liquid culture to start and a lot of it. I read in another post that one person used 10 to even 15 cc's of LC per jar for fast colonization, so I figured I'd give it a try.

I made injections in four slots and tilted the needle in each hole to inject in the center of the cake as well as on the edge of the cake. I think the LC may have sank mostly to the bottom because the mycelium is very rooted on the bottom of the jar, but less so on the top. I'll probably just wait till 3 weeks are up and put the jars under a really bright light for inspection. If I can't detect a definite contamination, I'll pop a jar open, see if it smells like mushrooms and look how well the mycelium colonized.


--------------------
No knowledge can be certain, if it is not based
upon mathematics or upon some other knowledge
which is itself based upon the mathematical
sciences.
  -Leonardo da Vinci (1425-1519)

Speak well of your enemies.  After all, you made them.

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Invisibleshroober
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Re: Is this mycelium started from liquid culture done yet? [Re: Mad River]
    #8364700 - 05/05/08 11:32 AM (16 years, 8 months ago)

Quote:

Mad River said:
Why did you use 10cc's per jar?




I use 7-10ccs of LC all the time on my quart jars of grain, it has never fucked up the water content. And the colonization times are amazingly fast.

I don't understand why everyone always says that much LC will fuck up the water content, I just don't see how they are reaching that conclusion. I've used that amount dozens of times on dozens of jars and have never had a problem.

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OfflineRisen_from_Ashes
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Re: Is this mycelium started from liquid culture done yet? [Re: shroober]
    #8364761 - 05/05/08 11:45 AM (16 years, 8 months ago)

i use a decent amount of lc in my grain to but less then 10cc's more like 4-6 ish


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Offlinesunkiing
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Re: Is this mycelium started from liquid culture done yet? [Re: shroober]
    #8364766 - 05/05/08 11:46 AM (16 years, 8 months ago)

I have 3 jars of karo that i used to inoculate a few pint jars and i only used 3-6 cc and its been 4 days now and so far just tiny white spots so im waiting it out :tongue:

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OfflineMad River
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Re: Is this mycelium started from liquid culture done yet? [Re: shroober]
    #8364778 - 05/05/08 11:50 AM (16 years, 8 months ago)

Quote:

shroober said:
Quote:

Mad River said:
Why did you use 10cc's per jar?




I use 7-10ccs of LC all the time on my quart jars of grain, it has never fucked up the water content. And the colonization times are amazingly fast.

I don't understand why everyone always says that much LC will fuck up the water content, I just don't see how they are reaching that conclusion. I've used that amount dozens of times on dozens of jars and have never had a problem.




I would think the water content on jars of grain would not be quite as delicate as water content in BRF jars?

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Invisibleshroober
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Re: Is this mycelium started from liquid culture done yet? [Re: Mad River]
    #8364786 - 05/05/08 11:53 AM (16 years, 8 months ago)

Yeah, very true, pint jars of BRF will be much more sensitive to water content than quarts of grain

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Offlinepothead_bob
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Re: Is this mycelium started from liquid culture done yet? [Re: shroober]
    #8374528 - 05/07/08 07:26 PM (16 years, 8 months ago)

What kind of grain do you use, spent brewers grain? Does that work for PF TEK and P. Cubensis mushrooms? Is it much better than using brown rice flour?

I should probably expand my experience in mushroom cultivation to optimize my results.


--------------------
No knowledge can be certain, if it is not based
upon mathematics or upon some other knowledge
which is itself based upon the mathematical
sciences.
  -Leonardo da Vinci (1425-1519)

Speak well of your enemies.  After all, you made them.

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Invisibleshroober
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Re: Is this mycelium started from liquid culture done yet? [Re: pothead_bob]
    #8374789 - 05/07/08 08:20 PM (16 years, 8 months ago)

Rye Berries or Wild Bird Seed

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Offlinepothead_bob
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Re: Is this mycelium started from liquid culture done yet? [Re: shroober]
    #8376773 - 05/08/08 08:38 AM (16 years, 8 months ago)

Checked the jars today and there is definatly a pink hue to all of them with some dark pink spots that sprouted over the last couple of days. Since all 9 jars are showing the same contamination pattern, I'm guessing that my liquid culture technique wasn't clean enough. Maybe some contam grew in the liquid culture, but I didn't see anything. Since I only have half a spore syringe left of Ecuador, I guess I'll probably just be starting from spore since I was able to do that correctly during my last grow. Better luck next time with the liquid culture, I guess.


--------------------
No knowledge can be certain, if it is not based
upon mathematics or upon some other knowledge
which is itself based upon the mathematical
sciences.
  -Leonardo da Vinci (1425-1519)

Speak well of your enemies.  After all, you made them.

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OfflineNeobean
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Re: Is this mycelium started from liquid culture done yet? [Re: pothead_bob]
    #8376784 - 05/08/08 08:44 AM (16 years, 8 months ago)

Quote:

pothead_bob said:
Checked the jars today and there is definatly a pink hue to all of them with some dark pink spots that sprouted over the last couple of days. Since all 9 jars are showing the same contamination pattern, I'm guessing that my liquid culture technique wasn't clean enough. Maybe some contam grew in the liquid culture, but I didn't see anything. Since I only have half a spore syringe left of Ecuador, I guess I'll probably just be starting from spore since I was able to do that correctly during my last grow. Better luck next time with the liquid culture, I guess.




Have you thought about taking the agar route? A clean wedge from an agar plate ==> clean LC.


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If y0u want s0meting gr0wn right, y0u g0tta gr0w it y0urself!!!

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Offlinepothead_bob
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Re: Is this mycelium started from liquid culture done yet? [Re: Neobean]
    #8381188 - 05/09/08 09:54 AM (16 years, 8 months ago)

No, I haven't really thought about agar at all. I guess I was avoiding that because I don't like ordering stuff online for this and I can't get the agar locally (at least I don't think). If I'm starting from spore, would I be able to use agar? Or is that just for doing tissue cultures?


--------------------
No knowledge can be certain, if it is not based
upon mathematics or upon some other knowledge
which is itself based upon the mathematical
sciences.
  -Leonardo da Vinci (1425-1519)

Speak well of your enemies.  After all, you made them.

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Offlinehottamali
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Re: Is this mycelium started from liquid culture done yet? [Re: pothead_bob]
    #8381213 - 05/09/08 10:06 AM (16 years, 8 months ago)

You can use Agar with spores, tissue, transfer existing agar with mycelium (an agar wedge ), and LC. Once you have some mycelium growing on the agar you could use that to make more agar dishes or more LC.

Edited by hottamali (05/09/08 10:28 AM)

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OfflineNeobean
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Re: Is this mycelium started from liquid culture done yet? [Re: pothead_bob]
    #8381214 - 05/09/08 10:07 AM (16 years, 8 months ago)

You can use spore syringes for Agar work. Agar should be available from your local health store (I think its used to lose weight as well lol) but if you have an asian supply store, you will find its cheaper there...


--------------------
If y0u want s0meting gr0wn right, y0u g0tta gr0w it y0urself!!!

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Offlinepothead_bob
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Re: Is this mycelium started from liquid culture done yet? [Re: Neobean]
    #8386244 - 05/10/08 05:30 PM (16 years, 8 months ago)

I do have an asian supply store where I live, actually. I'll stop and ask if they sell agar. Any ideas where I can get some petri dishes to put the agar in?


--------------------
No knowledge can be certain, if it is not based
upon mathematics or upon some other knowledge
which is itself based upon the mathematical
sciences.
  -Leonardo da Vinci (1425-1519)

Speak well of your enemies.  After all, you made them.

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Offlinemephist00
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Re: Is this mycelium started from liquid culture done yet? [Re: pothead_bob]
    #8386301 - 05/10/08 05:46 PM (16 years, 8 months ago)

you can find many things to use as petri dishes..

& you can order them off Mycosupply too i think (a sponsored site) tons of other websites sell them too


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