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Offlinejazzillion
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Incubation of jars in a cooler question.
    #8339303 - 04/28/08 08:43 PM (15 years, 10 months ago)

Im just looking for verification on my incubation setup. I have a Rubbermaid cooler with a reptile heat mat in it that is plugged to a control gauge. I have two separate batches of jars that I have in separate bags (the jars are in black garbage bags put into brown paper shopping bags). I have the two batches stacked in the separate bags on top of each other, so that they are not too close to the heat mat. Is this okay? I heard of contamination jump. Should I check the jars more often? Is it just bad practice to stack? Can it cause heat build up?

Thanks.


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Re: Incubation of jars in a cooler question. [Re: jazzillion]
    #8339331 - 04/28/08 08:47 PM (15 years, 10 months ago)

They will produce some of there own heat, so dont get them to hot...Room temp is just fine...


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Invisiblegrymmtymm
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Re: Incubation of jars in a cooler question. [Re: jazzillion]
    #8339358 - 04/28/08 08:49 PM (15 years, 10 months ago)

yes, keep them away from the heat pad

no, you can stack them if you want but i have never found it beneficial or harmful, probably best not to do so though..

yes, possibly heat could build up but worse is that you could block gas exchange..

yes, check jars often, especially the closer they get to full colonization..

really no need to put them into a garbage bag then a paper bag, no bags necessary....


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InvisibleRickster
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Re: Incubation of jars in a cooler question. [Re: jazzillion]
    #8339367 - 04/28/08 08:50 PM (15 years, 10 months ago)

There is no need for an elaborate setup for incubating jars. I just put then in my oven or closet, wherever is most convenient. As far as contams jumping from jar to jar I have never heard of it. As long as they have proper filters for gas exchange and are dry you should be good! Usually my failure is due to lack of sterility!

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Offlinejazzillion
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Re: Incubation of jars in a cooler question. [Re: Rickster]
    #8339421 - 04/28/08 09:02 PM (15 years, 10 months ago)

The thing is where I live it's still pretty cold and our house stays about 65 degrees on average (cooler in the morning), so I didn't expect a good colonization process. I do have masking tape and foil over the jar lids. Should I remove them out of the bags, and just place them at the bottom of the cooler, no heat necessary? The lids are secured tightly. Do I need to take some venting measures?

Thanks for the advice.


--------------------
When it rains, it spores :shroompick:

"Consciousness is the Universe recognizing itself." Once we perceive that everything is conscious we can then ask, "How does consciousness take all these varied forms?" - The Primacy of Consciousness by Peter Russell

All works of poster are of absolute fiction to be used for no other purpose but amusement.

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Offlinejazzillion
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Re: Incubation of jars in a cooler question. [Re: Rickster]
    #8339505 - 04/28/08 09:16 PM (15 years, 10 months ago)

What type of filters?


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When it rains, it spores :shroompick:

"Consciousness is the Universe recognizing itself." Once we perceive that everything is conscious we can then ask, "How does consciousness take all these varied forms?" - The Primacy of Consciousness by Peter Russell

All works of poster are of absolute fiction to be used for no other purpose but amusement.

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Re: Incubation of jars in a cooler question. [Re: jazzillion]
    #8339531 - 04/28/08 09:20 PM (15 years, 10 months ago)

If you need an incubator, I'd go with a TiT setup. Heat pads tend to get too hot and dry out jars that are in direct contact with them. The TiT setup provides gentle, even heat. The other thing it the garbage bags you're using may suffocate your jars by cutting off gas exchange.


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InvisibleDCRC184
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Re: Incubation of jars in a cooler question. [Re: FooMan]
    #8339550 - 04/28/08 09:26 PM (15 years, 10 months ago)

I'm assuming that you have a layer of dry verm as a contaminant barrier so..
First, you should get rid of the garbage bags and remove the masking tape from the holes to allow for gas exchange. I would keep the foil on the lids as long as they are loose enough for gas exchange.
Then you should put them in a TiT setup like FooMan said.

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Offlinejazzillion
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Re: Incubation of jars in a cooler question. [Re: DCRC184]
    #8339585 - 04/28/08 09:33 PM (15 years, 10 months ago)

I do have a layer of dry verm. So I'll remove masking tape, loosen the lids, but is it okay to keep them in the cooler? My first jars have been taking awhile, this might be the culprit. I have a heat mat regulator, so it has been a constant 75. I'm not pushing it. I just can't really deal with any new expenditures.


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When it rains, it spores :shroompick:

"Consciousness is the Universe recognizing itself." Once we perceive that everything is conscious we can then ask, "How does consciousness take all these varied forms?" - The Primacy of Consciousness by Peter Russell

All works of poster are of absolute fiction to be used for no other purpose but amusement.

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InvisibleDCRC184
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Re: Incubation of jars in a cooler question. [Re: DCRC184]
    #8339628 - 04/28/08 09:43 PM (15 years, 10 months ago)

I would put the heat pad on the bottom of the cooler and rig up a way to keep the jars a few inches up off the pad.

Keep the lids tight. What i meant was for you to make sure the foil covering the lids is somewhat loose.

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OfflineRogerRabbitM
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Re: Incubation of jars in a cooler question. [Re: DCRC184]
    #8339661 - 04/28/08 09:50 PM (15 years, 10 months ago)

Let your jars colonize in the open. Sealing them up with plastic or in a container can cause the dry verm filter to get damp, ruining its effectiveness. I strongly dislike 'incubators'. I'd suggest a high bookshelf or on top of a cabinet. It's always warmer near the ceiling.
RR


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Offlinejazzillion
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Re: Incubation of jars in a cooler question. [Re: RogerRabbit]
    #8339702 - 04/28/08 09:58 PM (15 years, 10 months ago)

Do you just store your jars in a bin of some sort with the lids (covered with foil) fully exposed to the room's atmosphere? I have cats and thought that recipe was just asking for contamination. Not to mention the temp in my house varies between night and day pretty drastically. It appears that some of the dry verm on top of my first set of jars has become a little moist as it is. Any advice?


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OfflineRogerRabbitM
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Re: Incubation of jars in a cooler question. [Re: jazzillion]
    #8339736 - 04/28/08 10:04 PM (15 years, 10 months ago)

Leave them in the open and get the foil off. The foil is to prevent water that drips off the top of the boiling pot from getting into the jars. Once you remove jars from the sterilizer, you're supposed to remove the foil. Here's how I colonize jars.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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InvisibleDCRC184
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Re: Incubation of jars in a cooler question. [Re: jazzillion]
    #8339753 - 04/28/08 10:08 PM (15 years, 10 months ago)

65 degrees (out in the open) isn't ideal. Instead you should shoot for around 80 degrees (in the cooler).

RR knows his stuff, but i think it would be best to keep the jars in the cooler (maybe open the cooler a little bit)
As long as they are undisturbed, some moisture in the verm layer shouldn't be a major problem.

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