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Offlinegrod31
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Registered: 12/06/07
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pan petris
    #8333682 - 04/27/08 10:33 AM (15 years, 9 months ago)

Four days ago i swabbed two petri dishes with Pan subhanburi spores from a print labeled 1/08... so now I'm looking at them and nothing... i have never had to wait 4 days to see growth on a dish before(usually if no myc then a contam of some sort), four days seems like a while, dose this happen? should i have hydrated the spores ?.
I am only trying to get clean growth on the plate not an isolate i have read isolates from pans are hard to work with and many people don't get one they are happy with.
after i get a decent amount of growth should i mix sterile water up in the petri to make some myco laden water or should i cut chunks out and put them in my jars ?
which way would be the least contam resistant ?


--------------------
Back the tape up.  I need it again!
Let it roll!  Just as high as the
fucker can go!  And when it comes
to that fantastic note where the
rabbit bites its own head off, I
want you to  THROW THAT FUCKING
RADIO INTO THE TUB WITH ME!
    Not me.  It would blast you through
the wall stone dead in ten
seconds and they'd make me explain
VVVVV Free Myco Thread


Edited by grod31 (04/27/08 08:45 PM)


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Offlinegrod31
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Registered: 12/06/07
Posts: 2,077
Loc: New York
Last seen: 15 years, 16 days
Re: pan petris [Re: grod31]
    #8335311 - 04/27/08 07:34 PM (15 years, 9 months ago)

anyone ?


--------------------
Back the tape up.  I need it again!
Let it roll!  Just as high as the
fucker can go!  And when it comes
to that fantastic note where the
rabbit bites its own head off, I
want you to  THROW THAT FUCKING
RADIO INTO THE TUB WITH ME!
    Not me.  It would blast you through
the wall stone dead in ten
seconds and they'd make me explain
VVVVV Free Myco Thread


Edited by grod31 (04/27/08 07:55 PM)


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Offlinecanid
irregular meat sprocket
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Loc: looking for zeebras, n. c...
Last seen: 1 month, 15 days
Re: pan petris [Re: grod31]
    #8336194 - 04/27/08 11:34 PM (15 years, 9 months ago)

yeah, i've had plates inoculated with dried spores take over a week to show. these days i follow germination at 640x or so with the scope.


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Attn PWN hunters: If you should come across a bluing Psilocybe matching P. pellicolusa please smell it.
If you detect a scent reminiscent of Anethole (anise) please preserve a specimen or two for study and please PM me.


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Offlinemedicine man
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Re: pan petris [Re: canid]
    #8336244 - 04/27/08 11:52 PM (15 years, 9 months ago)

If your sterile it doesn't which way you go. I like LC but I also make some petri dishes to use in jars if I want a good isolation for the LC. It is a little slower for RGS jars but for BRF cakes, it's like magic water.


Edited by medicine man (04/28/08 09:14 AM)


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