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ademordna31
Registered: 04/03/17
Posts: 14
Last seen: 4 years, 19 days
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Re: Mycelium on Agar Picture Thread *DELETED* [Re: FunGuy77]
#24419213 - 06/19/17 10:47 PM (6 years, 7 months ago) |
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Post deleted by ademordna31
Reason for deletion: no
Edited by ademordna31 (08/22/19 12:04 AM)
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Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,266
Last seen: 11 hours, 20 minutes
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Re: Mycelium on Agar Picture Thread [Re: ademordna31]
#24430924 - 06/24/17 06:01 AM (6 years, 7 months ago) |
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Morchella snyderi 05-10-17

Morchella snyderi 06-24-17, the same dish



http://mushroomobserver.org/274559
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lipa

Registered: 07/24/07
Posts: 2,684
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What are your plans with the sawdust/grain mixture?
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Sporulator


Registered: 03/08/11
Posts: 1,643
Loc: Europe
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Re: Mycelium on Agar Picture Thread [Re: lipa]
#24431648 - 06/24/17 12:54 PM (6 years, 7 months ago) |
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Cool, Alan becomes a cultivator! 
Morchella costata

After the mycelium colonized the substrate completely, the nutrients are translocated to the upper nutrient poor part of the substrate and the mycelium turns into one massive layer of sclerotium.


Edited by Sporulator (06/24/17 01:51 PM)
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Quadman
Challenged


Registered: 04/23/16
Posts: 2,529
Loc: IL
Last seen: 1 year, 3 months
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Re: Mycelium on Agar Picture Thread [Re: Sporulator]
#24432214 - 06/24/17 04:36 PM (6 years, 7 months ago) |
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Just started messing with morel. They are cool and very interesting. Nice pics guys!
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Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,266
Last seen: 11 hours, 20 minutes
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Re: Mycelium on Agar Picture Thread [Re: lipa]
#24435055 - 06/25/17 05:49 PM (6 years, 7 months ago) |
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Quote:
lipa said: What are your plans with the sawdust/grain mixture?
I am going to go to Mexico and it's staying in the USA...so it'll probably just sit there. Maybe it'll make sclerotia and maybe I'll be able to talk some people into doing some g2g transfers.
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Steevo
Just a cog in the death wheel



Registered: 03/02/17
Posts: 2,702
Loc: Here and There
Last seen: 1 year, 9 months
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Its tough to get a good pic through these mini rounds
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Mycelium on Agar Picture Thread [Re: Steevo]
#24435639 - 06/25/17 10:50 PM (6 years, 6 months ago) |
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Unused and tossed to the side Pan cambo plate, shit is aggressive as fuck.
-------------------- JOIN THE POW WOW
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DrMushroom
Human Farmer


Registered: 04/28/12
Posts: 722
Loc: Australia
Last seen: 21 days, 5 hours
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Cubensis Escondido on non-sterilized peroxide MEA agar, made with 2mL 6% peroxide before boiling, and another 3mL 6% right before pouring.
Xfered from a huge mass of mycelium that crept far up the side of a grain jar displaying desirable traits. The jar itself was inoculated with a very small slither of a 9 month old multispore agar culture.
Overuse of peroxide is why i think it looks like this, and why the first 10 days saw no growth whatsoever.
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
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Re: Mycelium on Agar Picture Thread [Re: BlimeyGrimey] 2
#24606587 - 09/05/17 05:54 PM (6 years, 4 months ago) |
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Hey family, I'm in the middle of my first isolation process and wanted to share this.

Most of those are from a ms agar plate. These are fourteen transfers in. Any thing marked cl-... Is from a clone. Do you think I can isolate on my next transfer on some of these?
If anyone wants an up close picture of any of these, just ask. I'll back light it and post it. I'd love to know what you folks think.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Probably a few more transfers on all of those to get an isolate. I would just work with what you have now then take some awesome clones
Nice work tho killer job
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,807
Loc: Canada
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Re: Mycelium on Agar Picture Thread [Re: bodhisatta]
#24606653 - 09/05/17 06:14 PM (6 years, 4 months ago) |
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Definitely killer work. Dedicated. Once you have isolates the real job begins. But there is probably something awesome in there for sure.
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
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Re: Mycelium on Agar Picture Thread [Re: Pastywhyte] 1
#24606714 - 09/05/17 06:30 PM (6 years, 4 months ago) |
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Thanks guys. I knew It would be a lot of work but holy fuck are there more genetics in a drop or two than I thought possible. I'm sure that some great shit is already available but I'm going to see it through to isolation. I can't help myself.
All of those are in shoeboxes and monos at the moment as well and I'll be cloning the best for sure.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,807
Loc: Canada
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Yep I remember the first time I tried to isolate PE. Unbelievable how much is in a drop. A learning experience for sure.
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ShroomyToons
Be Calm and Grow On



Registered: 01/16/17
Posts: 3,867
Loc: Rebel Flag
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You can go to a lt of grain on what you have there. Once you are working from clones you will have better luck with repeating what you want. I super isolated AA+ and ended up with A+. It was kind of musing. Nice fruits though, and now I actually have both strains. lol.
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
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Quote:
verum subsequentis said: Hey family, I'm in the middle of my first isolation process and wanted to share this.

Most of those are from a ms agar plate. These are fourteen transfers in. Any thing marked cl-... Is from a clone. Do you think I can isolate on my next transfer on some of these?
If anyone wants an up close picture of any of these, just ask. I'll back light it and post it. I'd love to know what you folks think.

Transfer 17.
Bottom row is mostly clones
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
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Re: Mycelium on Agar Picture Thread [Re: bodhisatta]
#24688564 - 10/06/17 01:47 PM (6 years, 3 months ago) |
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I'm almost there. I've never done this (isolation) before but it seems like the myc is taunting me. Most of my ms plates have, what seem to be, monoculture sectors that I could transfer out and be "done". But there is always a faster more rizo sector (or ten) just staring me down.
I don't know if they are actually healthier and worth pursuing or if they are just forced to try harder due to the monoculture networks presence surrounding them. I don't even know if that makes sense.
Any thoughts.
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Demitrifruit
Stranger


Registered: 05/27/17
Posts: 12
Last seen: 5 years, 11 months
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hello to all contributing to this thread. I just discovered it and have learned allot from the pages I have read thus far. I have a question to those who may have some insight. I recently did my first tissue sample to two agar dishes, along with a pin which I split in half into two other dishes. A total of four. I practiced the most ocd of sterile methods that one is able to with only a still air box, flame sterilized scalpel, and plenty of lysol and alcohol between each inoculation of agar. I know that I was sterile because two dishes are contam and bacteria free. However, the two which show green mold spots and bacteria are showing a bit of a pattern. I saran wrapped the first two from the pin transfer on top of each other, re-sterilized, and inoculated the next two from tissue and wrapped them the same way. What I found was that only the TOP dishes on each two dish stacks grew mold. The bottom dishes in each two dish stack are perfectly clean and healthy. Anyone have any ideas on what caused only the top dishes to contaminate when sterilization was done to the highest degree. keep in mind the two types of transfers were done separately so I know that if one dish was contaminated it wouldn't have cross contaminated. And even if it did somehow, that wouldn't explain the top dish contam trend. thanks to all that may can help shed some light on the issue. MushLove - Demitrifruit
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Om Namo Shivaya


Registered: 05/03/15
Posts: 749
Last seen: 7 days, 14 hours
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Re: Mycelium on Agar Picture Thread [Re: Demitrifruit]
#24693580 - 10/08/17 10:17 AM (6 years, 3 months ago) |
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I've recently put supposed bisporus spores onto agar and was curious if anyone has opinions on how this looks. I've read and seen pictures of bisporus mycelium being quite grey, but this is very white mycelium so far.

The left plate is spores from a vendor syringe, the right is transfer from the center. I'm wondering if the grey patch of growth at the 12 o clock position is the bisporus and the white growth is maybe something else?
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