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Sporulator
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Re: Mycelium on Agar Picture Thread [Re: Feasoghorm] 3
#25995296 - 05/15/19 10:59 PM (4 years, 10 months ago) |
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Quote:
Timboslice said: Seriously does look like a bad case of psoriasis
Most sclerotia do so.
Morchella importuna
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myceliups
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Re: Mycelium on Agar Picture Thread [Re: Sporulator] 1
#25995323 - 05/15/19 11:25 PM (4 years, 10 months ago) |
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Quote:
Sporulator said:
Quote:
Timboslice said:
Quote:
Sporulator said:
That looks wack.
Close up
Your cincts always come out the same that mine do
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Sporulator
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Re: Mycelium on Agar Picture Thread [Re: myceliups]
#25995396 - 05/16/19 01:04 AM (4 years, 10 months ago) |
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Quote:
myceliups said: Your cincts always come out the same that mine do
Probably a similar agar medium.
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Feasoghorm
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Re: Mycelium on Agar Picture Thread [Re: Sporulator]
#25995409 - 05/16/19 01:33 AM (4 years, 10 months ago) |
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Hehe yeah see, more skin irritation. At first it made me thnk of a cat with two assholes. But im not sure where i wud have seen that b4
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Ferather
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Re: Mycelium on Agar Picture Thread [Re: Feasoghorm]
#25995616 - 05/16/19 06:09 AM (4 years, 10 months ago) |
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@ Timboslice
You're a bit rude and unusual, my first reaction was to Google images of 'Panaeolus cinctulus on agar'. It turned out Sporulator's images look odd, but sort of normal for Panaeolus cinctulus.
I guessed it focused on mini sclerotia more than colonizing.
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MacMerdin
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Re: Mycelium on Agar Picture Thread [Re: Ferather] 2
#25996014 - 05/16/19 11:35 AM (4 years, 10 months ago) |
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Cinctulus invitro fruiting on agar.
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Adas
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Re: Mycelium on Agar Picture Thread [Re: MacMerdin]
#25996035 - 05/16/19 12:01 PM (4 years, 10 months ago) |
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Quote:
MacMerdin said:
Cinctulus invitro fruiting on agar.
Holy shit! Didn't know that was even possible!
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Himinji
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Re: Mycelium on Agar Picture Thread [Re: MacMerdin]
#25996926 - 05/16/19 09:31 PM (4 years, 10 months ago) |
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What will cause fruiting in a agar culture?
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Sporulator
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Re: Mycelium on Agar Picture Thread [Re: Himinji] 4
#25997009 - 05/16/19 10:32 PM (4 years, 10 months ago) |
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Quote:
Himinji said: What will cause fruiting in a agar culture?
Concerning Panaeolus cinctulus: If your agar medium is rich in nutrients you will get bluing sclerotia. If the nutrient content of your agar medium is moderate to low you will get shrooms.
Here's an example...when the mycelium meets the nutrient rich layer in the jar consisting of oat flakes it starts to form sclerotia:
Edited by Sporulator (05/16/19 11:43 PM)
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Feasoghorm
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Re: Mycelium on Agar Picture Thread [Re: Sporulator]
#25997104 - 05/16/19 11:50 PM (4 years, 10 months ago) |
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Panaeolus are a bit beyond my ability at the moment.
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Ferather
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Re: Mycelium on Agar Picture Thread [Re: Sporulator]
#25997335 - 05/17/19 05:21 AM (4 years, 10 months ago) |
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@ Sporulator
Is that growing upwards, and the new layer on top is the nutrient rich media? If so, it's got bluer the higher up it went, with more nutrition.
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Sporulator
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Re: Mycelium on Agar Picture Thread [Re: Ferather] 3
#25997465 - 05/17/19 07:28 AM (4 years, 10 months ago) |
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Quote:
Ferather said: @ Sporulator
Is that growing upwards, and the new layer on top is the nutrient rich media? If so, it's got bluer the higher up it went, with more nutrition.
No, it grows from top to bottom. The substrate consists of sterilized composted horse manure and a layer of oat flakes in the middle.
When the mycelium reaches the oat flakes layer it thickens and starts to form sclerotia whereas the mycelium in the horse manure layers doesn't change.
My intention is to work out if it is possible to get some nice big Panaeolus cinctulus stones.
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Ferather
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Re: Mycelium on Agar Picture Thread [Re: Sporulator]
#25997492 - 05/17/19 07:51 AM (4 years, 10 months ago) |
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I see, very good. I was wondering why it's producing antioxidants so far down, but now that makes sense, top to bottom. Very interesting work, it's visibly producing less bruising the further down it forms sclerotia (lower O2).
Would you mind trying a wedge onto T-Gel, if you don't mind, even cheap black tea works. T-Gel contains no sugar, starch or cellulose *, so results will be interesting.
* No leaf, cellulose with leaf.
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In addition, it looks like it gets more, or does better, on the grain over the composted horse manure. When I put a truffle onto WL-Tek it just colonized (wispy) and fruited as mushrooms.
Perhaps it prefers proteins over ureic and ammonical nitrogen?
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Edited by Ferather (05/17/19 08:05 AM)
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Sporulator
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Re: Mycelium on Agar Picture Thread [Re: Ferather] 3
#25997576 - 05/17/19 08:59 AM (4 years, 10 months ago) |
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I will give it a try. Did you adjust the pH value of the T-Gel agar medium?
The nitrogen source seems to be a very important factor in the life cycle of Panaeolus cinctulus. A few years ago I used an agar medium based on Glutamine and Glycine as a source of nitrogen and I got one big sclerotia nugget on the agar. No aerial hyphae at all were visible.
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Ferather
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Re: Mycelium on Agar Picture Thread [Re: Sporulator] 1
#25997600 - 05/17/19 09:11 AM (4 years, 10 months ago) |
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I use hard water, which is about pH 7.4, it contains calcium bicarbonate. The end pH of T-Gel should be about 6.0-6.5, varying with the tea and age.
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Wow, that's an amazing sclerotia, nice work. You can also do T-Gel + gelatin (pig skin, 240B, animal proteins).
Here is Cubensis knots on modded T-Gel: 1.6g Black Tea (0.6g Soluble), 0.8g Sucrose, 0.04g MG.
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Thanks for the great info.
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Sporulator
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Re: Mycelium on Agar Picture Thread [Re: Ferather] 1
#25997670 - 05/17/19 09:53 AM (4 years, 10 months ago) |
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Thanks for all the interesting informations!
It looks that agar medium low in nutrients leads to fructification for a number of mushrooms.
I have to workout if that works also for Panaeolus cyanescens .
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Ferather
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Re: Mycelium on Agar Picture Thread [Re: Sporulator]
#25997694 - 05/17/19 10:06 AM (4 years, 10 months ago) |
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It could be a spend or store scenario, my guess is with low nutrition, the pan mycelium will try and sporulate. I would say with higher nutrition (or more usable materials) it stores resources for later.
Other mycelium might fruit only with higher nutrition or certain nutrients. If I enrich an oyster grow, I actually get more fruit mass.
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Ferather
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Re: Mycelium on Agar Picture Thread [Re: Ferather] 1
#25999186 - 05/18/19 06:13 AM (4 years, 10 months ago) |
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Azure vendor wood spawn sample to T-Gel (plain, no ME, no additives):
Visible multi-genetics, I will be isolating 9 o'clock. The azure mycelium is producing enzymes, new cells, sugars, and other components, from the decay of phenols-other. Phenols-other are not composed of sugar units (like starch, cellulose), the mycelium utilizes phenols-other as a 'carbon' source, converted.
The black tea extract also contains all the necessary, non carbon, macro-micro nutrients needed to grow out healthy.
Isolated growth should be visibly uniform, and radial, like these Cubensis images.
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T-Gel recipe and data, here.
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Sporulator
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Re: Mycelium on Agar Picture Thread [Re: Ferather]
#25999200 - 05/18/19 06:29 AM (4 years, 10 months ago) |
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That's very interesting, thanks for sharing. I have to try that with Psilocybe ovoideocystidiata. No ME means no malt extract?
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Ferather
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Re: Mycelium on Agar Picture Thread [Re: Sporulator]
#25999208 - 05/18/19 06:37 AM (4 years, 10 months ago) |
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Yes, it's optional, and it's included in the recipe. I'm intentionally not adding sugar, to prevent unwanted infection. The oxidation process of tea also kills bacteria, very few can survive. It's effectively pro mycelial.
Also note ME is made by adding enzymes to grain, it's better to use grain water. That way the mycelium produces all the enzymes needed for grain.
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If you isolate on grain water agar, the mycelium should do very well on hydrated grain.
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For giggles, here is Cubensis growing on calcium carbonate, and a transfer of the carbonate to low nutrient paper *.
There is bruising top right of image 5, and other areas (active).
* Not suggested, enrich.
Edited by Ferather (05/18/19 07:05 AM)
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