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Invisibleda_Vinci7
Stranger
Registered: 03/17/02
Posts: 22
Psilocybe cubensis "cambodian" on rye, cased with coir/verm.
    #832759 - 08/21/02 11:42 AM (14 years, 3 months ago)

Hello all, I have a friend (let?s call him "Dave"), that has taken a interest in mycology(purely for educational value) and has succesfully cultivated using various BRF based substrates/coir casings many times in the past. Dave has just this past spring begin to work with bulk substrates- grain and straw. The last four months have been a real learning process for him. Frustrated by recent failures he became even more determined to succeed. He knew he was going to have to change his way of thinking in order to have success on the next level. His old setup, while sufficient for small amounts of substrate, was not allowing enough air exchange for larger/deeper bulk substrates. His latest attempt has included a total redesign of his system and deeper level of understanding of the mushroom life cycle. Dave would like to share his recent work to help others that may be struggling with the same problems he has faced "moving to the next level"

Dave?s latest project was started just about two months ago, he has taken A LOT of pictures throughout a large portion of it. It will take some time for me to post everything to catch up to where Dave?s project is now, as I have a job and a life unlike Dave. Well, with that being said let?s get started....

Dave will skip on the important but lengthy details, if anybody wants to know exactly how Dave does something just ask. He started with 3 Qt jars of rye that were innoc. w/ 2cc?s apiece of a Cambo syr. Growth was evident in all 3 by Day 5. They were shaken on Days 6 and 12. This is what they looked like on day 18......

Closeup of Jar#2...

Closeup of Jar#1...

Tools that come in handy w/ jars....


Like I said before if anybody wants details on anything, ask.

Dave?s plan for part 2 is to take his 3 master jars of rye and turn them into 15 QT jars. At this time Dave would also point out that he had 10 1/2pint jars of Thai K.S. on a mix of BRF/RyeFlour/Quinoa(innoc at same time as master QT?s)....

Dave has had trouble with this strain all summer for various reasons(he feels he may have gotten a bad batch/strain as 2 of his syr. were contaminated and the other 2 never seemed quite right. These cakes proved no different- while they seemed to colonize just fine when they were crumbled and cased they contaminated quickly. Here?s a couple of pictures of the cakes after removal from the growing area...

.
The contamination was discovered on the 6th day after casing pretty much by accident when Dave deep scratched a small area and noticed the green. No green was noticed anywhere in the casing layer, only on the pieces of cakes. Whatever it was spread through those pieces very fast, almost all were covered with it.

Back to the rye, here?s a shot of the incubating jars after transfer from masters...

Two more pictures taken approx. 1 week after transfers....



Here?s a picture of the type of tray that will be used for casing....


Out of 15 jars, Dave ended up throwing out 2 of them, 1 had a green spot on the bottom and the other was very slow colonizing and didn?t look quite right to him. His plans were to put 5 Qt. jars in Tray#1, and 4 QT. jars apiece in #2&3. Dave uses a combination of coir/vermiculite/chalk/gypsum for his casing(sterilized in oven for 90Min at 350F). After cooling, he applied approx. 3/4" layer to each tray.
The trays were then put into the incubation/fruiting terraniums. The air pump ran on a timer so that it came on 4 times a day for 1/2 hour at a time. Dave found that this worked fine for his trays, providing some gas exchange for the case run without chasing out too much humidity or CO2. During the case run, the trays were checked on twice a day - am/pm- to check temps/humidity and look for problems.
Here?s a picture of tray?s #1/2 on Day 5 after casing.....

A closeup of Tray#1 on Day 5......

Tray?s #1/2 on Day 7.....

Tray #3 on Day 7.....

A must have tool imho that comes in really handy for pasteurization and taking substrate temps.....


The next two pictures are a comparison of tray#1 on Day 7 and Day 9....



Tray?s #1/2 on Day 9.....

Closeup of Tray #2 - Day 9....


Here are some good closeups of mycelium growth in casing-Day 9.....



Tray#3 - Day 9......


Day-12 Photo?s......
Tray?s #1&2....

Tray #3....

Closeup Tray #1

Mycelium Closeup?s.....



Negative Images......



On Day 12 the air pump was switched on a 1.5 hr on/ .5 hr off schedule and the fruiting lids were placed on both cases. The 12 hr on/off lighting schedule was started the next day. During the case run the temps stayed between 78.4 to 85.4(Low/HI for entire caserun)

Dave has overloaded me with pictures to post , so I?ve decided to split this grow log into two parts. The second part will be strictly fruiting phase to completion.



Edited by Annom (02/07/05 10:21 AM)


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OfflineGreen_Velvet
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Registered: 07/26/02
Posts: 412
Loc: La La Land
Last seen: 7 years, 10 months
Re: Cambo's on Rye(Updated 8/22) [Re: da_Vinci7]
    #835726 - 08/22/02 12:15 PM (14 years, 3 months ago)

Damn looking nice man. Looks like its about time to initiate fruiting eh?


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OfflineKingBolete
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Registered: 06/14/02
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Loc: Gulf Coast
Last seen: 13 years, 4 months
Re: Cambo's on Rye(Updated 8/22) [Re: da_Vinci7]
    #837085 - 08/22/02 11:04 PM (14 years, 3 months ago)

Hey there,

Juan/Javier sees the air lines but where do they go and have you changed the setup during the process? Juan/Javier sees a blue device laying in the perlite in the bottom of the tub and later it is missing and some sort of air diffusion device is mounted on the walls of the tub? What is going on in the there for humidity, etc.? Anyway, nice looking setup you have created...especially the air pump box. Is it as quiet as it looks in the pictures?

Juan/Javier is not sure why he is asking questions as he has basically wasted all the spawn mycelum he has worked with...and not by contamination either. It just sorta sits there, does not spread (colonize) through any of the substrate mediums he has tried and eventually dies away. Creating it in BRF/verm, rye berries, etc is a snap. Getting it to do anything past there has been a real challenge and he is not sure if it has anything to do with his technique so much as it has to do with "other issues". It looks like for indoor cultivation he may have to wait until he is in a different physical location; either that or figure out how to keep his friends and neighbors out his "science projects". He has a feeling he has been getting everything he does too wet and is going to make corrections for that this next pass of EightGrain/BRF/verm pint jars. He has found that EightGrain and TenGrain hot cereal mixes from Uncle Bob's Red Mill work good along with Uncle Bob's Red Mill BRF which is from organic brown rice. All are free of any additives and are in the organic section of the grocery store. Of course rye berries work very well and are great for doing grain transfers. He found that only a small spoonful into each new jar is all that is required. Actually a single colonized rye berry kernel would mostly likely work fine. . Juan/Javier will continue his "science projects" until he is successful and satisfied and has a good bounty of shrooms in hand...heheh!!

Best regards,

King Bolete


Edited by KingBolete (08/22/02 11:08 PM)


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Invisibleda_Vinci7
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Registered: 03/17/02
Posts: 22
Re: Cambo's on Rye(Updated 8/22) [Re: KingBolete]
    #841851 - 08/25/02 09:20 AM (14 years, 3 months ago)

Hello, yes Dave decided to change his setup during the caserun. The blue stones are bubble stones that he bought. They are still in the case but are not hooked up to an airline. He didn't know how his humidity level would hold up with this setup so he bought them just in case he needed them to provide humidity. He has since found out that he really doesn't need them as the humidity levels stay high enough with just the casings and a good misting of the sides when needed. Dave has dry perlite in the bottom of his cases to catch and hold any excess water. The airlines you see are attached to bubble walls that are held by suction cups to the side of the cases just above the casings(one on each side - opposite ends). The bubble walls are approx. 14 " long and were poked with a sewing needle once every 3/4" or so in a straight line to provide an even line of air output across the length of the wall. Dave decided that to have the fresh air pumped in just above the top the fruiting containers was best so the excess CO2 would be push out the check valve line at the bottom of the case. When he tested his airpump(250Litres of air per hour/per outlet) he discovered that it pushed sufficient air to power 2 of the 14" bubble walls per airline, and yes the airpump is very quiet - even on the high setting you would have to be pretty close to hear it, and with a fan or air purifier on it's next to impossible to hear unless your right next to it.

As far as your problems go, sorry to hear it btw, it may be that you have a gas exchange problem, as Dave had perfectly colonized grain earlier this year only to have it stall during the case run. He was using the same size substrate containers that he uses now but had a much smaller terranium with no provisions for Co2 escape in either the terranium or the substrate container. Hence the setup change. So far his problems have been solved. Good luck!


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OfflineKingBolete
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Registered: 06/14/02
Posts: 235
Loc: Gulf Coast
Last seen: 13 years, 4 months
Re: Cambo's on Rye(Updated 8/22) [Re: da_Vinci7]
    #844124 - 08/26/02 12:42 PM (14 years, 3 months ago)

Hey there,

Looks like Dave's stuff is going to happen!! I assume you got the bubble walls at a pet supply or pet supply section of a larger general consumer store (i.e. Wally World etc.). By the way, are those Pan. Cambodginiensis? They are supposed to be quite potent....

Juan/Javier has a few unused aquarium's he may bring online to try some more work with...forturnately some of the cake pieces that Juan/Javier put into an outside tek type situation are starting to come to fruitation so to speak. Juan/Javier was quite nicely surprised by a boomer coming up Thursday morning that was ready to pick Saturday morning. Juan/Javier got a nice 2" cap with medium mocha chocolate center with nipple turning tan to the edges. He wisely made two prints from this cap and after doing the yard work Saturday, he plucked the cap from it's print making and commenced to scarf it up. He then took an interesting shower, shaved (mirror, mirror on the wall...hehehe!) and settled in to watch "Kiss of the Dragon" with Jet Li while quite mesmerized. The cap turned out to be extremely potent and Juan/Javier was quite satisfied with his first experience with these items. He noticed another area that was getting ready to give a him a nice size outdoor bounty...he has plenty of material to work with and will continue to work on his cultivation techniques.

Best regards,

King Bolete


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation >> Mushroom Cultivation Archive >> Grow Logs

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