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Offlinehighc
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no-growers
    #8300498 - 04/18/08 10:48 AM (15 years, 11 months ago)

i have 6 cakes i knocked up bout 2 weeks ago. only one jar shows growth in one spot. but the growth is crazy fast. the other five don't smell and just are not showing anything.

procedure: i ousted my are flamed the needle and with only one hole uncovered at a time i inoculated as quickly as possible. no glove box was used but the room has one of those oreck air purifiers right next to where i was.

at first i thought bad syringe since i did not but from sponsor.....i know but too late now. But since at least one jar shows healthy growth that can't be.

then i was thinking when i inoculated i did not look at the jar only the syringe moving in haste. so if i stuck the needle right in the substrate instead of up on the glass how long could it take to show on the outside?


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OfflineGuffeyJon
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Re: no-growers [Re: highc]
    #8300544 - 04/18/08 11:03 AM (15 years, 11 months ago)

Did you pack down the cake or leave it lose?
-air purifiers are bad they just spread contamination.
where and at what temp are you storing your jars? Do you have an incubator?

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Offlinehighc
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Re: no-growers [Re: GuffeyJon]
    #8300556 - 04/18/08 11:07 AM (15 years, 11 months ago)

Quote:

GuffeyJon said:
Did you pack down the cake or leave it lose?
-air purifiers are bad they just spread contamination.
where and at what temp are you storing your jars? Do you have an incubator?




its snug. ive made tons of cakes all the same. verm is a lil looser. double tub incubator set at 72 degrees.


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OfflineGuffeyJon
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Re: no-growers [Re: highc]
    #8300568 - 04/18/08 11:10 AM (15 years, 11 months ago)

if your sure that it wasn't packed to tight i would wonder if you made the syringe or if maybe it got to hot during shipping?

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InvisibleBlutjager
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Re: no-growers [Re: highc]
    #8300572 - 04/18/08 11:11 AM (15 years, 11 months ago)

Did you wait until the next day to inoculate them after cooking them to make sure they were 100% cool all the way to the center of the jars ??

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Offlinehighc
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Re: no-growers [Re: Blutjager]
    #8300640 - 04/18/08 11:29 AM (15 years, 11 months ago)

Quote:

Blutjager said:
Did you wait until the next day to inoculate them after cooking them to make sure they were 100% cool all the way to the center of the jars ??




that may be another problem...i waited 10 days after pc then hit up. inbetween they were in a airtight spot. i think this may caused a problem. but i had jars that sat for 2 weeks and grew perfect. IDK


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