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Offlineperosiste
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Copper in a Liquid Culture
    #8213286 - 03/30/08 01:42 AM (16 years, 9 months ago)

Most of my research on liquid cultures talks about putting something in the jar to break up the myc so that you can get it into a syringe. So I was thinking what about copper, either a section of wire of a few bbs.

Copper has antimicrobial properties and should be able to help prevent/fight of contams.

Does anyone know why this wouldnt work? Would the copper also kill the myc or would the myc react the with the copper to form a harmfull chemical.


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InvisibleSmushroom
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Re: Copper in a Liquid Culture [Re: perosiste]
    #8213298 - 03/30/08 01:49 AM (16 years, 9 months ago)

I would imagine that putting bare copper in water would corrode it over time and it may eventually break it up. I have no clue on whether mushrooms require any if at all copper in their systems, I highly doubt it. I would imagine too much copper in the water would do way more harm than good.

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Offlineperosiste
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Re: Copper in a Liquid Culture [Re: Smushroom]
    #8213477 - 03/30/08 04:44 AM (16 years, 9 months ago)

I'm not considering putting copper in the water as a supplement the mushrooms, I am only considering this as a way to fight contams.

In order to test this I think will try the following experiment.

Take 6 half pint jars

Make enough liquid culture to fill all 6 jars in one batch

PC 3 of them and and in one place an extreme amount of copper(layer of copper bbs on the bottom of the jar) in the second place 3-5 copper bbs and in the third no bbs.

For the second set of 3 jars repeat the set up with the bbs but do not PC the jars

Knock up all six jars with the same syringe.
wait for results.

Repeat for both spore syringes and a liquid culture syringe with myc in it.

Give me a week or two and I will post the results here, in the mean time if someone else is bored and wants to run this at the same time then please do so. I would like to compare the results with what I get.


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OfflineMYSTIQUE
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Re: Copper in a Liquid Culture [Re: perosiste]
    #8213492 - 03/30/08 05:10 AM (16 years, 9 months ago)

You still need three controll jars with no copper in them and pced or you wont have anything to compare you findings too.

You should also use a isolate to knock up the jars or the mysc inside will just show you how genetics can be diffrent from plant to plant.


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InvisibleSmushroom
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Re: Copper in a Liquid Culture [Re: MYSTIQUE]
    #8213567 - 03/30/08 07:19 AM (16 years, 9 months ago)

I know you aren't wanting to use it as a supplement but the myc will more than likey absorb some copper which may be more than it can handle and kill off the myc.

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Invisiblekiowa27
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Re: Copper in a Liquid Culture [Re: Smushroom]
    #8213680 - 03/30/08 08:37 AM (16 years, 9 months ago)

the copper bb that you can commonly buy for airguns are merely coated with a minute amount of copper, it would be much better if you're serious about it to use a few chunks of copper wire. it is always interesting to see the directions peoples inquiring minds take them in, keep us posted :smile:


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OfflineReedviper
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Re: Copper in a Liquid Culture [Re: kiowa27]
    #8213896 - 03/30/08 10:40 AM (16 years, 9 months ago)

i have seen a little piece of broken glass used successfully to break up the myc without any cotams, maybe u could try one with glass and one with copper.. ive also seen marbles used, i myself would go with the broken piece of glass, but i really dont need anything to break up my myc, it just rips apart when i spin it in the jar

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InvisibleantiPock
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Re: Copper in a Liquid Culture [Re: Reedviper]
    #8213951 - 03/30/08 10:57 AM (16 years, 9 months ago)

How about silver? I think it has even better anitmicrobial properties.

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OfflineFamiliar Gnome
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Re: Copper in a Liquid Culture [Re: antiPock]
    #8214468 - 03/30/08 01:06 PM (16 years, 9 months ago)

In the best breweries; copper is used throughout the brewing process and contributes no ill effects to the brewing or fermentation process.

It would stand to reason that it wouldn't harm your L.C. either.

A previous post in this same thread by AntiPock; mentions silver. Very interesting idea as well. Colloidal Silver would be another interesting experiment to try too. Not as an agitator, rather as an adjunct or base to the L.C.

I don't know how the silver's antibacterial properties or effects on enzymes and other proteins would effect the L.C.

As I said; interesting experiment.

Please keep us all posted with all of these tests.


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Offlineperosiste
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Re: Copper in a Liquid Culture [Re: Familiar Gnome]
    #8214869 - 03/30/08 02:27 PM (16 years, 9 months ago)

Mystique you mention using 3 control jars with no copper in them. I don't label it specifically as a control but in my vaguely written experimental procedure I mention two jars with no copper one pced one not. Do you think that I need more control samples or do you think that those would be sufficient.

Also can someone point me in the direction of how I can identify what contaminated LCs look like or how I can otherwise know when a jar has turned south.

I do agree with you that I should use an isolate to prevent genetic differences from showing through. As soon as I have a fruit to isolate then I will repeat this test again in the mean time all I have are syringes and myc so I will use myc extracted from a grain jar for the time being.

I appreciate all the interest and support on this one and I will keep everyone posted.


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Re: Copper in a Liquid Culture *DELETED* [Re: perosiste]
    #8214890 - 03/30/08 02:30 PM (16 years, 9 months ago)

Post deleted by dumbum39

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OfflineMagicMaker
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Re: Copper in a Liquid Culture [Re: dumbum39]
    #11685415 - 12/20/09 10:39 AM (15 years, 1 month ago)

was there any verdict on this, i have been trying to use a penny as an agitator as i didnt have any glass, but now as i write this post i im thinking i should have just broken a bottle or something anyway i have had no luck with spore germination and was wondering if maybe the penny could be inhibiting?  (in a LC)

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Re: Copper in a Liquid Culture [Re: MagicMaker]
    #13041793 - 08/12/10 02:19 PM (14 years, 5 months ago)

Yes, yes, I know. This is over 7 months old but I wanted to tell of my experience.

All I wanted to say was that I also tried putting pennies in my LC to help break of the mycellium (pennies were just the first thing I thought of) and it it has prevented four jars (two from one batch and two from a separate batch) from germinating at all so yes, I would say that something about copper does prevent mycellium from growing. Just for future reference if anyone else wants to try it...don't.


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Re: Copper in a Liquid Culture [Re: cdawg3731]
    #13041985 - 08/12/10 02:54 PM (14 years, 5 months ago)

Interesting... I want to experiment now.


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OfflineIssius
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Re: Copper in a Liquid Culture [Re: cdawg3731]
    #13042107 - 08/12/10 03:15 PM (14 years, 5 months ago)

You just had some bad luck.  I use pennies in my LC's.  I'll try to post some pics of them when I get back to my apartment (very soon).

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OfflineIssius
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Re: Copper in a Liquid Culture [Re: Issius]
    #13042181 - 08/12/10 03:33 PM (14 years, 5 months ago)

I lied.  Just looked at my LCs and the ones I have pennies in failed to grow, while the ones I have nothing in have gobs and gobs of mycelium floating around. 

Should have realized that was the culprit....btw, I have 8 jars.  4 with pennies and 4 with nothing.  The 4 with nothing all are gorgeous, the ones with pennies have no growth or the beginning of growth that then failed.

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OfflinePfffffff
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Re: Copper in a Liquid Culture [Re: Issius]
    #13042413 - 08/12/10 04:29 PM (14 years, 5 months ago)

Sometimes I see posts that make me not feel stupid for doing shit without searching first.  This is one of those times.  Copper FTL!


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OfflineRogerRabbitM
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Re: Copper in a Liquid Culture [Re: Pfffffff]
    #13042668 - 08/12/10 05:29 PM (14 years, 5 months ago)

Copper is fungicide.

If you'll use a 14 gauge needle, the mycelium will pass right through it.
RR


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OfflineMagicMaker
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Re: Copper in a Liquid Culture [Re: RogerRabbit]
    #13044918 - 08/13/10 04:11 AM (14 years, 5 months ago)

So BTW the cheapest thing i think to use instead of pennies is take a glass bottle, and break it.... now you have 100 or more inert agitators, just be careful with them cause they be sharp!

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