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OfflinetelefunkenU47
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Registered: 08/03/02
Posts: 102
Loc: cartoon planet
Last seen: 13 years, 3 months
sterile cloning suprise
    #819203 - 08/16/02 03:40 AM (14 years, 3 months ago)

this is amazing. a while ago i tried the fresh stem, peroxide and water in a coffee grinder tek. 8 jars were innoculated. after 1 week: nothing. after 2 weeks: nothing. i gave up on them and didn't look at them for another 10-11 days and then i found my suprise. as i went to throw them out, i found that 7 of 8 were in some stage of colonization. some are 10% and some are 80%. almost all of the growth is very solid and rhizomorphic (more so than i have ever seen with spores). how is this possible? my understanding was that it would take 3-5 days to see growth, but it took 3 times as long as it would have with spores. isn't this supposed to cut down on incubation times?


--------------------
Central Scrutinizer:
This is the CENTRAL SCRUTINIZER...
That was Joe's first confrontation with The Law.
Naturally, we were easy on him.
One of our friendly counselors gave him
A do-nut...and told him to
Stick closer to church-oriented social activities.

-Frank Zappa's "Joe's Garage"


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OfflineMacey Howard
Formally MOE HOWARD
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Registered: 07/03/99
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Post deleted by Moe Howard [Re: telefunkenU47]
    #819212 - 08/16/02 03:51 AM (14 years, 3 months ago)



--------------------
Hugs and Kisses!


Edited by Macey Howard (08/16/02 03:52 AM)


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OfflineKilla420
Alien Lover
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Registered: 06/26/02
Posts: 1,055
Loc: Down South
Last seen: 17 days, 4 hours
Re: sterile cloning suprise [Re: telefunkenU47]
    #819474 - 08/16/02 06:57 AM (14 years, 3 months ago)

I had kind of like the same thing when I cloned a wild shroom. I did the blender tek but only inaculated 2 half pint jars. After about a week there were no signs of anything so I just left them there and a couple of days later I finally noticed that they were growing. I think it has something to do with the h2o2 that has to breakdown or something but I could be wrong. I too had really nice looking myclieum, better than I have seen from spores and after a couple of myclieum transfers to other jars, they are in the final stage of colognizing my 2 quart jars. I cant wait till they are done b/c I have never cloned anything yet especially a wild cube.


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OfflinetelefunkenU47
numerator
Registered: 08/03/02
Posts: 102
Loc: cartoon planet
Last seen: 13 years, 3 months
Re: sterile cloning suprise [Re: Macey Howard]
    #819694 - 08/16/02 08:26 AM (14 years, 3 months ago)

pf substrate (verm., brf, yadda yadda yadda)

1/2 pint jars.

incubation temps were kept between 75-80.

always in the dark except for when i would pull them out and curse them for not starting colonization within 3-5 days.

minimum of 2.5 cc's per jar although some received up to 4 cc's.

sorry i didn't include this info. in the earlier post, but i was late for work and i was trying to eat cocoa pebbles and type at the same time.


--------------------
Central Scrutinizer:
This is the CENTRAL SCRUTINIZER...
That was Joe's first confrontation with The Law.
Naturally, we were easy on him.
One of our friendly counselors gave him
A do-nut...and told him to
Stick closer to church-oriented social activities.

-Frank Zappa's "Joe's Garage"


Post Extras: Print Post  Remind Me! Notify Moderator
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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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