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OfflineAnnoA
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Cloning Pan. cyanescens
    #818977 - 08/16/02 12:24 AM (14 years, 3 months ago)

When cloning species where the mushroom flesh is thin and thus it?s difficult to obtain a clean tissure, it?s allways a good idea to cut out several pieces of tissure. They can even be placed at the same dish.



As you can see, out of 5 pieces placed at the dish, only 2 show healthy growth, the other 3 are contaminated with bacteria.
I will transfer the 2 healthy spots to each 1 dish tomorow.


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Offlinezeronio
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Re: Cloning Pan. cyanescens [Re: Anno]
    #819208 - 08/16/02 03:47 AM (14 years, 3 months ago)

Very nice.
The dishes have to big to make it work. I usually use small dishes and have to make 10 or more to get few without contamination.
Do panaeolus grow on peroxidated media?


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OfflineAnnoA
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Re: Cloning Pan. cyanescens [Re: zeronio]
    #819408 - 08/16/02 06:27 AM (14 years, 3 months ago)

>Do panaeolus grow on peroxidated media?
Yes.


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Re: Cloning Pan. cyanescens [Re: Anno]
    #819779 - 08/16/02 08:53 AM (14 years, 3 months ago)

why don't u use some antibiothic agar? it really helps a lot...


Edited by CLuB99 (08/16/02 08:56 AM)


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Anonymous

Re: Cloning Pan. cyanescens [Re: Anno]
    #819964 - 08/16/02 10:15 AM (14 years, 3 months ago)

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OfflineAnnoA
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Re: Cloning Pan. cyanescens [Re: CLuB99]
    #820241 - 08/16/02 11:40 AM (14 years, 3 months ago)

>why don't u use some antibiothic agar? it really helps a lot...
As you can see it?s not really needed for this....

It?s good if you have contaminated rare spores though....


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Re: Cloning Pan. cyanescens [Re: ]
    #820254 - 08/16/02 11:42 AM (14 years, 3 months ago)

>Do you always parafilm?
Never.
What I do is I cut a 1 inch piece off a polyetyilene kitchen wrap foil and use this.

It?s the same stuff as http://www.glad.com/plasticwrap_faq.html .


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Re: Cloning Pan. cyanescens [Re: CLuB99]
    #821368 - 08/16/02 04:49 PM (14 years, 3 months ago)

Ive found antibiotics are selective in what bacteria they are able to prevent - better of just using a few petris and quickly transfering to peroxdated agar - works well for me...


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