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I thought this might be of interest.Even though it was done by an imaginary playmate in another universe....
In August 2000,several strains of cubensis were grown out on rye.One or two colonized rye grains of each was used to innoculate more rye in tiny (pinky sized) screw top test tubes.When the tubes were fully colonized,they were refrigerated.
In August 2002, 3 grains of this rye (EQ strain in this case) were removed and placed onto agar.Fresh mycelial growth on the grain was noted at 2 days,and leap off to the agar noted by 4 days.
So,this seems a viable (pun intended) method.One benefit over agar cultures is that a dozen or so rye grains occupies very little space.If carefully placed into little foil packages,one could have a pretty extensive culture library that fits inside of a film canister!
Hopefully my imaginary playmate will return in 2 more years and find out how the remaining cultures have held up after 4 years.It also may start some new tests to see how they fare without refrigeration.
Re: An Experiment In Long(ish) Term Culture Storage [Re: aural] #817271 - 08/14/02 11:08 AM (14 years, 6 months ago)
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