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OfflineDERRAYLD
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Pastuerized Poo/straw life
    #816224 - 08/13/02 10:25 PM (18 years, 8 months ago)

I pastuerized some poo 2 days ago, left it covered in my shower to strain.
Forgot to use it last night, was wondering if it would still be useable tonight or if i`d have to pastuerize it again?


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Re: Pastuerized Poo/straw life [Re: DERRAYLD]
    #816261 - 08/13/02 10:41 PM (18 years, 8 months ago)

Once it's properly pasturized and at the correct moisture content, you could put it into a large zip-lock bag and store it for months in ready-to-use condition.

*EDIT* ?You haven't showered for over 2 days? PHEW! And I thought horse poo smelled!!


--------------------
================================================



"Have some congratulatory drugs." - C. Montgomery Burns

I'll probably always do drugs, so that just contributes to the addiction to The Shroomery... It's a vicious circle of bliss. :tongue2:

TM™ :cool:


Edited by TripMeister (08/13/02 10:43 PM)


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OfflineAnnoA
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Re: Pastuerized Poo/straw life [Re: TM]
    #816290 - 08/13/02 11:05 PM (18 years, 8 months ago)

>Once it's properly pasturized and at the correct moisture content, you could put it into a
>large zip-lock bag and store it for months in ready-to-use condition.

Hmmm, don?t think so. What you forget is that by pasteurizing alone many bacteria and some molds still survive.
If you leave the pasteurized substrate uninoculated, it will "spoil" eventually.

But not after 2 days.


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Re: Pastuerized Poo/straw life [Re: Anno]
    #816305 - 08/13/02 11:12 PM (18 years, 8 months ago)

In reply to:

But not after 2 days.


I think you mean not BEFORE 2 days. Probably not before 10 days at least.


--------------------
================================================



"Have some congratulatory drugs." - C. Montgomery Burns

I'll probably always do drugs, so that just contributes to the addiction to The Shroomery... It's a vicious circle of bliss. :tongue2:

TM™ :cool:


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OfflineAnnoA
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Re: Pastuerized Poo/straw life [Re: TM]
    #816310 - 08/13/02 11:15 PM (18 years, 8 months ago)

Yes, I ment not yet after 2 days.


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OfflineDERRAYLD
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Re: Pastuerized Poo/straw life [Re: TM]
    #816313 - 08/13/02 11:16 PM (18 years, 8 months ago)

Thanks gents.
What I needed to know.
More poo-straw to spawn puts a smile on my face.
Hope the PUerto Ricans do better this time.


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OfflineDERRAYLD
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Re: Pastuerized Poo/straw life [Re: DERRAYLD]
    #816317 - 08/13/02 11:18 PM (18 years, 8 months ago)

Anno?? you been okay with all this flooding at the moment?
Not flooding near you?
Saw it on the news for the first time last night.


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OfflineAnnoA
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Re: Pastuerized Poo/straw life [Re: TM]
    #816320 - 08/13/02 11:19 PM (18 years, 8 months ago)

>Probably not before 10 days at least.

Probably much sooner.
Read TMC, page 124 towards the bottom. Big letters.


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OfflineAnnoA
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Re: Pastuerized Poo/straw life [Re: DERRAYLD]
    #816322 - 08/13/02 11:20 PM (18 years, 8 months ago)

>Not flooding near you?
Luckily not.


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Re: Pastuerized Poo/straw life [Re: Anno]
    #816334 - 08/13/02 11:27 PM (18 years, 8 months ago)

I'll take your word for it. I always spawn it the day after pasturization anyway.


--------------------
================================================



"Have some congratulatory drugs." - C. Montgomery Burns

I'll probably always do drugs, so that just contributes to the addiction to The Shroomery... It's a vicious circle of bliss. :tongue2:

TM™ :cool:


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OfflineAnnoA
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Re: Pastuerized Poo/straw life [Re: TM]
    #816336 - 08/13/02 11:30 PM (18 years, 8 months ago)

Here you have it:

Paul Stamets  and J.S Chilton: The Mushroom Cultivator , page 124

Spawning Methods

Spawning methods, like spawn itself, have evolved over the years. As late as
1950 Agaricus brunnescens growers customarily planted walnut sized pieces
of manure spawn or kernels of grain spawn in holes poked into the compost at
regular intervals. Using this method spawn running was slow, and areas far from
the inoculum were more susceptible to invasion by competitors. The full potential
of grain spawn was not realized until the development of "mixed spawning". The principle of mixed spawning is the complete and thorough
mixing of the grain kernels throughout the substrate. In this manner all parts
of the substrate are equally inoculated, resulting in the most rapid and complete
colonization possible.

The standard spawning rate used by Agaricus growers is seven liters/ton
of compost or one quart/8 sq. ft. If spawn is readily available and cheap, it
is advantageous to use high spawning rates which lead to more rapid colonization.
It is also advantageous to break up the grain spawn into individual kernels
the day before spawning. If the spawn is fresh, the grain should break apart
easily. If the spawn can not be used when fresh, it should be refrigerated at
38?F.

The basic principle of spawn running is the same regardless of the type of mushroom
or substrate. COLONIZATION MUST PROCEED AS RAPIDLY AS POSSIBLE TO PREVENT OTHER
ORGANISMS FROM BECOMING ESTABLISHED
. Once the mushroom mycelium becomes dominant,
natural antibiotics secreted into the substrate inhibit competitors. To prevent
invasion by competitors it is important that spawning take place under carefully
controlled hygienic conditions. Fungus gnats in particular must be excluded,
and for this purpose a tight, well sealed working area is best. This area and
all tools should be disinfected one day prior to spawning with a 10% bleach
solution. When using disinfectants be sure your skin is protected and avoid
breathing any fumes.   



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Re: Pastuerized Poo/straw life [Re: Anno]
    #816354 - 08/13/02 11:44 PM (18 years, 8 months ago)

Thanks Anno, but you know I've got the book. You didn't have to go through the trouble of scan/ocr, but I suppose it's a good post for those who don't have it.


--------------------
================================================



"Have some congratulatory drugs." - C. Montgomery Burns

I'll probably always do drugs, so that just contributes to the addiction to The Shroomery... It's a vicious circle of bliss. :tongue2:

TM™ :cool:


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OfflineAnnoA
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Re: Pastuerized Poo/straw life [Re: TM]
    #816363 - 08/13/02 11:50 PM (18 years, 8 months ago)

>You didn't have to go through the trouble of scan/ocr
Already had this chapter.

> but I suppose it's a good post for those who don't have it.
That?s why I posted it.


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OfflineDERRAYLD
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Re: Pastuerized Poo/straw life [Re: Anno]
    #816456 - 08/14/02 01:13 AM (18 years, 8 months ago)

Helped me on my way


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Invisiblematts
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[Re: DERRAYLD]
    #891730 - 09/18/02 07:24 PM (18 years, 7 months ago)



Edited by matts (09/18/02 07:25 PM)


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Invisiblemickey_rourke
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Re: Pastuerized Poo/straw life [Re: matts]
    #891735 - 09/18/02 07:26 PM (18 years, 7 months ago)

Pasteurizing allows for the proliferation of beneficial bacteria which compete with unwanted contaminants.


--------------------
"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five


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Invisiblematts
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[Re: mickey_rourke]
    #891741 - 09/18/02 07:30 PM (18 years, 7 months ago)



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Re: Pastuerized Poo/straw life [Re: matts]
    #891814 - 09/18/02 08:07 PM (18 years, 7 months ago)

Unfortunately, I'm not a microbiologist, so I will have to provide somewhat of a rudimentary answer. When material is pasteurized, the heat generated kills off bacteria that could compete with the mushrooms for food and even harm the mycellium. The remaining bacteria does not compete with the mycellium for food (or harm it for that matter) and can even make additional nutrients available.

Hope that clarifies things somewhat...


--------------------
"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five


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OfflineXiC_clone
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Re: Pastuerized Poo/straw life [Re: mickey_rourke]
    #892217 - 09/18/02 11:16 PM (18 years, 7 months ago)

I heard from Road that if you re-pasteurize something, you are killing off that beneficial bacteria. Is there any truth to that? Not that I would ever doubt road, but I see ppl posting "Yea, I pasteurize'd it 3 times, let it sit for a week, and am going to pasteurize it again" (I am streching it a bit, but just to provide a example)


-XiC


--------------------
Don't soak your WBS dummy! There's an =>easy<= way.
For Your Health!


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Re: Pastuerized Poo/straw life [Re: XiC_clone]
    #892221 - 09/18/02 11:19 PM (18 years, 7 months ago)

Sterilizing will certainly kill all the bacteria, but I wasn't aware that multiple pasteurizations would kill the organisms that were resistant to the temperatures pasteurization maintains. Maybe Roadkill will chime in and clarify this for us.


--------------------
"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five


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Re: Pastuerized Poo/straw life [Re: mickey_rourke]
    #892225 - 09/18/02 11:20 PM (18 years, 7 months ago)

I linked him the thread, hopefully he will clear this up =)


-XiC


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For Your Health!


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OfflineDERRAYLD
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Re: Pastuerized Poo/straw life [Re: XiC_clone]
    #892237 - 09/18/02 11:27 PM (18 years, 7 months ago)

It`s not necessary to get Roadkill's advice.
Come on.
He knows what he`s talking about, YEs.
But so do alot of us here as well.
I`ve grown out enough bulk tubs to be able to give my 5c worth.
I have re-pasteurised poo 3 times and the spawned it and it worked perfectly for me.


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Re: Pastuerized Poo/straw life [Re: DERRAYLD]
    #892238 - 09/18/02 11:28 PM (18 years, 7 months ago)

Yes, this is what I feel too.  I was hoping Roadkill would chime in and clarify what he said.. :wink:  I don't want to speak for him. 


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"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five


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Re: Pastuerized Poo/straw life [Re: XiC_clone]
    #892263 - 09/18/02 11:51 PM (18 years, 7 months ago)

I am a studying microbiologist/chemist. I have not come into contact with detailed info reguarding the practice of pasteurisation. As I understand it, the more aggressive organisms tolerate natural conditions (normal temp ranges), while the organisms that tolerate higher temp conditions are not as aggressive...therefore pose a lesser threat to the mycelium of a standard species (P. cube).

I have personally found that p[asteurisation does not work for the micro-organisms in my area. I have had several consecutive pasteurisations go bad. When I sterilised my dung in a PC I found colonisation speed to increase and contamination to cease.

I have not examined this phenomenone in detail, but will continue to PC my dung.

I believe the idea behind pasteurisation is that beneficial organisms will remain that will either help to fight foreign contams or to induce fruiting in certain species. According to Stamets however, P. cubes do not require any bacteria or other organisms to induce healthy fruiting. I don't think that sterilising dung will decrease the successfull spawn run of P. cubes, but actually help in certain environments.


I am drunk and reached real far up my shizznit hole to provide the previous info. I am sure Anno will come along to correct me shortly (the dude is crazy smart).

Joshua


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Re: Pastuerized Poo/straw life [Re: XiC_clone]
    #892878 - 09/19/02 09:39 AM (18 years, 7 months ago)

Quote:

I heard from Road that if you re-pasteurize something, you are killing off that beneficial bacteria. Is there any truth to that?



I never said that!
What I said was >> I would never sterilize horse poo!
The reason I wouldn't sterilize it is this... you would lose some beneficial bacteria.

The purpose of pasteurization is to kill or neutralize all harmful organisms.

I have never had to re-pasteurize any poo... so I really don't know if it can be done.
I always spawn my poo the day after pasteurizing it.


Derrayld said
Quote:

"It`s not necessary to get Roadkill's advice.
Come on.
He knows what he`s talking about, YEs.
But so do alot of us here as well." 



Very true Derrayld...and I don't have the answers to everything...I'm not an Expert!

In fact my motherboard went out on my computer....that is why I haven't posted in over 12 hours.
Thank God a friend brought over his spare computer for me to borrow.

What would you do if I dissapeared for a week or longer?
Answer :
Ask others that know what they are talking about.
There are lots....I repeat lots of people that know what they are talking about on this site.

I don't have the answers for everything!
Hell...I don't think I'll ever have the answers to everything!!
I read alot...and try to learn all that I can about mycology.
Life is a learning experience.....learn all that you can!
I still ask a few questions myself... and who do I ask...people like Anno, Joshua, Mushroom John...etc.


Joshua had a problem with pasturized poo and has been sterilizing his poo and hasn't had any problems.
I'm sure he will go far in life....He has the ambition and drive!

So who truely knows what works best?

Do what works best for you!!!

:smile:


     


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineDERRAYLD
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Re: Pastuerized Poo/straw life [Re: Roadkill]
    #893043 - 09/19/02 10:56 AM (18 years, 7 months ago)

Informative and precise as usual Roadkill.


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