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Invisiblelegallyhomeless
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Registered: 07/01/05
Posts: 4,051
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Tissue to LC Question
    #8159546 - 03/17/08 09:59 PM (15 years, 10 months ago)

The other night I got some baby bottle from walmart. I filled the tips with silicone and let it dry. I then added 200ml water and 6ml karo surup.

I microwaved the mix for 5 mins. Let it cool and bit and then microwaved for another 5 mins. This brought the water to a boil each time.

I had some trouble because when the water in the bottles was cooling it created a suction and kept sucking the baby bottle nipples into the bottle which I then had to empty and repeat the process.

After they were finally cooled down, I took a fresh mushroom from my monotub and riped it down the middle. I then took a scalple and took a small tissue sample, diped it in h202 for a second, then put it into the baby bottle.

My questions is...

Since this was a small mushroom, possibly, from the cracked corn spawn and low rH, but also possibly from genetics (EQ strain) will my LC be a clone of this mushroom? I asume so but I would hope that I can get better results if I use the LC.

I just made a few LCs for fun because I have never had a successful one. 1 bottle tissue, 1 bottle multispore.


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Invisiblenalyudi
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Re: Tissue to LC Question [Re: legallyhomeless]
    #8159627 - 03/17/08 10:14 PM (15 years, 10 months ago)

you'll get a clone of the one you took tissue from. you should have used your purrdyest one. by purrdy i mean normal, large, and healthy.


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InvisibleSmushroom
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Registered: 02/02/05
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Re: Tissue to LC Question [Re: nalyudi]
    #8160202 - 03/18/08 12:09 AM (15 years, 10 months ago)

Like he said, you will get a clone of the mushroom you took the sample from. However that doesn't mean that the resulting fruits will all be small. Size is more environmental than genetic (from a given strain).

Using clones for LC will help out later. Just like cloning or isolating on agar. Avoids the problems with multispore.

I have a LC going that I also took from a tissue sample and one going from a biopsy of a casing. By biopsy I mean I took a syringe needle and jabbed it into the substrate (after removing a small chunk of the side) and put that into an LC.

The cloned one took off a lot faster but isn't a speedy grower, not compared to MS ones I have did in the past. Not sure why.


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Invisiblenalyudi
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Re: Tissue to LC Question [Re: Smushroom]
    #8160216 - 03/18/08 12:14 AM (15 years, 10 months ago)



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Invisiblelegallyhomeless
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Re: Tissue to LC Question [Re: nalyudi]
    #8162198 - 03/18/08 01:04 PM (15 years, 10 months ago)

Ah thanks nalyudi. I knew I read something like that but I couldn't find it. The mushroom was healthy, just small, due to my impatience


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