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OfflineDragonChaser
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Registered: 04/27/06
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RogerRabbits Sporalator
    #8148868 - 03/15/08 09:57 AM (16 years, 10 months ago)

I can't find the thread that had it... maybe I'm not spelling it right?
Thought it was 'sporalator', or maybe 'sporulator'...
Anyone care to help me out and link me up?
It sounds like a nice way to make syringes and skip the print making stage.


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My name is Mud

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OfflineDragonChaser
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Re: RogerRabbits Sporalator [Re: DragonChaser]
    #8152063 - 03/16/08 12:20 AM (16 years, 10 months ago)

No one can help me on this?


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My name is Mud

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OfflinePsuper
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Registered: 02/12/08
Posts: 2,878
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Re: RogerRabbits Sporalator [Re: DragonChaser]
    #8152122 - 03/16/08 12:47 AM (16 years, 10 months ago)

~Sporulate or sporulation is one thing....but you're talking about some kind of tek or something?


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OfflineDragonChaser
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Re: RogerRabbits Sporalator [Re: Psuper]
    #8153087 - 03/16/08 12:06 PM (16 years, 9 months ago)

its a jug thing, with an injection port at the bottom, and the lid has a hook in it... he pressure cooks it, and then takes a clean cap, opens it real quick, sticks the cap on the hook, closes it and the spores drop into the bottom making spore solution which is then sucked into a syringe through the injection port....
No one knows what I'm talking about?


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My name is Mud

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OfflineMana
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Registered: 03/10/08
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Re: RogerRabbits Sporalator [Re: DragonChaser]
    #8153133 - 03/16/08 12:20 PM (16 years, 9 months ago)

Mana can help.  :thumbup:

http://www.shroomery.org/forums/showflat.php/Number/4152603#4152603

Quote:

agar said:
You dont need to inject 2 or 3 ml of spore solution. Any amount (so long as it has a few viable compatable spores in it) will work. More - simply gets the action going faster, IMHO.

I certainly don't advocate WASTING spore solution. At the same time, I prefer to use the content of a spore laden syringe at one sitting, so I do not have partial syringes laying around.

Once - you master mush/cult & PRINT or SPORE SOLUTION MAKING you generaly have more spores in print or solution form - than a single person (or small group) could EVER USE.

I often use a SPORALATOR to make spore solution:



1/2 or 1 gallon wide mouth jar (Sun Tea type) - with spigot removed & replaced with an injection / asperation port - that reseals, installed in the hole in the jar - where the spigot was.

Lid altered with a gas exchange filter & install a clip of some sort to hold a cap suspended with a stainless steel treble hook through the caps stem area - inside the container.

Fill container with 1000 / 1500 / 3000 ml water (depending on size of container) & PC the whole container with water inside it 20 minutes @ 15 psi &  allow to cool to room temp.

When ready for use, harvest a large cap (veil about ready to tear), inside a G/B or F/H - swab cap top with h202, remove veil, peirce cap stem area with sterile treble hook, open container & attach cap to clip, which hangs over sterile water & will drop spores directly into it (repeat once or twice or more w/same strain cap).

The result is 1000 / 1500 / 3000 ml of spore solution that can be asperated through the injection / asperation port on the side of the jar via a sterile syringe.

Test first syringe on agar/petri/plate to insure spore solution contains no contaminates & (if not) presto - you now have a capacity to fill 100 / 150 / 300  syringes out of a single spore laden solution container - in aseptic conditions.  :thumbup:




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Everybody lies, but it doesn't matter since nobody listens.

How to become immortal: Read this signature tomorrow and follow its advice.

Edited by Mana (03/16/08 12:31 PM)

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OfflineDragonChaser
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Re: RogerRabbits Sporalator [Re: Mana]
    #8153250 - 03/16/08 12:56 PM (16 years, 9 months ago)

Fucking righteous dude. Thanks.


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My name is Mud

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