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OfflineFingerfoods
Pale Blue Dot
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Registered: 11/14/06
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Last seen: 12 years, 6 months
WBS Jars Mystery
    #8148041 - 03/14/08 11:48 PM (16 years, 10 months ago)

Ok so about two weeks ago I inoculated 3 WBS jars that were about 15% full in order to make liquid culture from them later, with a multi spore B+ syringe. Its been two weeks and I still havent seen any growth the only thing I cant think of that might have effected it were that

1. I let the jars cool over night in the PC and didn't shake them until the next morning when I pulled em out.

2. The room I left them in for the first week might have been a little over 85 degrees. But the jars still have a bit of moisture on the bottom so I dont think they are dried out.

What could be the the problem you think? I mean spore is pretty resilient shouldn't it be growing a little? Or should I be patient and keep waiting, I have never had to wait this long though. Could it be bad spore? I got it from Hawk eye though so I dont think so.


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Offline81Renaissance
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Registered: 11/01/06
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Re: WBS Jars Mystery [Re: Fingerfoods]
    #8148050 - 03/14/08 11:52 PM (16 years, 10 months ago)

Any FAE?


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"Every passing moment is another chance to turn it all around."

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OfflineMana
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Registered: 03/10/08
Posts: 203
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Re: WBS Jars Mystery [Re: Fingerfoods]
    #8148068 - 03/14/08 11:59 PM (16 years, 10 months ago)

Quote:

Fingerfoods said:
1. I let the jars cool over night in the PC and didn't shake them until the next morning when I pulled em out.

2. The room I left them in for the first week might have been a little over 85 degrees. But the jars still have a bit of moisture on the bottom so I dont think they are dried out.




Reason #1 won't make a difference as long as you didn't shake them after innoculating. Did you?
Reason #2 shouldn't make a difference unless there is a pool of water on the bottom.
How many CCs of inoculate did you use? Did you aim the spores against the edge of the jar?


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Everybody lies, but it doesn't matter since nobody listens.

How to become immortal: Read this signature tomorrow and follow its advice.

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OfflineFingerfoods
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Re: WBS Jars Mystery [Re: Mana]
    #8148091 - 03/15/08 12:11 AM (16 years, 10 months ago)

I did shake on two of the jars after inoculating I remembered on the third that I wasn't supposed too.

There is no pool of moisture on the bottom you can just tell by looking its a little moist. Also I aimed the spore against the edge and used about 3 CC of multi spore.

I used 3 layers of Tyvek this time to minimize risk of contams do you think this could be the cause? By not allowing enough FAE? Only thing is though shouldn't have grown decently with the air thats already in the jar for a while then stalled or something if that were the case?


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http://palebluedot.ytmnd.com/ watch this shrooming and youll never think the same about the world agian.

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OfflinePsuper
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Registered: 02/12/08
Posts: 2,878
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??? [Re: Fingerfoods]
    #8148139 - 03/15/08 12:32 AM (16 years, 10 months ago)

~Wait, wait...why don't you guys shake after inoculation of wbs? I don't remember anyone saying not to do this...~Pixie~


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Clinical Management of High Dose Psilocybin Sessions:
https://www.youtube.com/watch?v=FbHOTIqjZLk

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InvisibleFooMan
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Re: ??? [Re: Psuper]
    #8148156 - 03/15/08 12:39 AM (16 years, 10 months ago)

Shaking doesn't matter immediately after inoculation. I know some people don't do it so that they get good colonization at the inoculation points, then shake to spread the myc around after, but it certainly won't hurt to shake early on.


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Quick WBS Prep

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Offlinegrod31
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Re: ??? [Re: Psuper]
    #8148168 - 03/15/08 12:41 AM (16 years, 10 months ago)

" used 3 layers of Tyvek this time to minimize risk of contams do you think this could be the cause? By not allowing enough FAE?"

if you think thats the problam i would poke holes in all 3 layers w/ a sterile needle and put micropore tape on the top holes. im purrty fucked up rite now so feel free to flame if i said something stupid?:o)


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Back the tape up.  I need it again!
Let it roll!  Just as high as the
fucker can go!  And when it comes
to that fantastic note where the
rabbit bites its own head off, I
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    Not me.  It would blast you through
the wall stone dead in ten
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OfflinePsuper
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Re: ??? [Re: FooMan]
    #8148173 - 03/15/08 12:44 AM (16 years, 10 months ago)

~Thanks Foomanshroom; I didn't think it would.  I am following Doc's Tek and even asked about this a few days ago in some random thread.  I shook my jars well right after inoculation and will again once they are mostly colonized.  :smile2: ~Pixie~


--------------------
Clinical Management of High Dose Psilocybin Sessions:
https://www.youtube.com/watch?v=FbHOTIqjZLk

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Invisiblemb13
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Registered: 03/07/08
Posts: 108
Re: ??? [Re: Psuper]
    #8148180 - 03/15/08 12:46 AM (16 years, 10 months ago)

Shake once at 30%. Shake again at spawn. No more, or it'll add 2-5 days.

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OfflineMana
Substance ofSouls


Registered: 03/10/08
Posts: 203
Loc: Where Everything Good Is ...
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Re: ??? [Re: Psuper]
    #8148182 - 03/15/08 12:48 AM (16 years, 10 months ago)

Quote:

Psuper said:
~Wait, wait...why don't you guys shake after inoculation of wbs? I don't remember anyone saying not to do this...~Pixie~




Quote:

RogerRabbit said:
Shaking right after inoculation was a mistake. You should allow the spores to germinate in close proximity to each other so they can pair up and become dikaryotic. Monokaryotic mycelium is thinner and much slower growing than dikaryotic mycelium. By shaking the jar, you required it to colonize with the slower growing monokaryons.

It will recover, but be slower. Next time, shoot the spores down the side of the glass at the edges. Don't shake afterward for several days until 20% or so of the rye berries are colonized.
RR




Edited by Mana (03/15/08 12:55 AM)

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OfflinePsuper
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Registered: 02/12/08
Posts: 2,878
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Re: ??? [Re: Mana]
    #8148195 - 03/15/08 12:54 AM (16 years, 10 months ago)

Quote:

Mana said:

Quote:

RogerRabbit said:
Shaking right after inoculation was a mistake.  You should allow the spores to germinate in close proximity to each other so they can pair up and become dikaryotic.  Monokaryotic mycelium is thinner and much slower growing than dikaryotic mycelium.  By shaking the jar, you required it to colonize with the slower growing monokaryons.

It will recover, but be slower.  Next time, shoot the spores down the side of the glass at the edges.  Don't shake afterward for several days until 20% or so of the rye berries are colonized.
RR




To be honest I haven't done many experiments with this I just took his word for it. 




~Thanks Mana...I am going to have to try both ways now to  see this.  I appreciate the reply. :hug: ~Pixie~


--------------------
Clinical Management of High Dose Psilocybin Sessions:
https://www.youtube.com/watch?v=FbHOTIqjZLk

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InvisibleFooMan
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Re: ??? [Re: Mana]
    #8148398 - 03/15/08 03:07 AM (16 years, 10 months ago)

Quote:

Mana said:
Quote:

Psuper said:
~Wait, wait...why don't you guys shake after inoculation of wbs? I don't remember anyone saying not to do this...~Pixie~




Quote:

RogerRabbit said:
Shaking right after inoculation was a mistake.  You should allow the spores to germinate in close proximity to each other so they can pair up and become dikaryotic.  Monokaryotic mycelium is thinner and much slower growing than dikaryotic mycelium.  By shaking the jar, you required it to colonize with the slower growing monokaryons.

It will recover, but be slower.  Next time, shoot the spores down the side of the glass at the edges.  Don't shake afterward for several days until 20% or so of the rye berries are colonized.
RR








RR has you guys hypnotized :eek:  :lol:


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Quick WBS Prep

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OfflineMana
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Registered: 03/10/08
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Re: ??? [Re: FooMan]
    #8148971 - 03/15/08 10:48 AM (16 years, 10 months ago)

:justdontknow:


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Everybody lies, but it doesn't matter since nobody listens.

How to become immortal: Read this signature tomorrow and follow its advice.

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OfflineFingerfoods
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Re: ??? [Re: Mana]
    #8149078 - 03/15/08 11:27 AM (16 years, 10 months ago)

So you guys think its the fact that I shook that I am not seeing any growth?


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OfflineMrIzzy11B
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Re: ??? [Re: Fingerfoods]
    #8149086 - 03/15/08 11:30 AM (16 years, 10 months ago)

i shook mine and im starting to see growth i would just wait a bit longer before i freak out to much you will be ok

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OfflineMana
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Posts: 203
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Re: ??? [Re: Fingerfoods]
    #8149142 - 03/15/08 11:48 AM (16 years, 10 months ago)

http://www.shroomery.org/forums/showflat.php/Number/6403281#6403281

This guy had the same problem and also didn't have good lids for gas exchange. They should start growing soon anyway like Cowboy says but 3 filters might be too much. 1 tyvek filter has always worked for me. I don't think the problem was shaking since there's one jar you didn't shake and it's not growing either.


--------------------
Everybody lies, but it doesn't matter since nobody listens.

How to become immortal: Read this signature tomorrow and follow its advice.

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Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Injection Grain Bag   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   MagicBag.co All-In-One Bags That Don't Suck   Myyco.com Golden Teacher Liquid Culture For Sale   Left Coast Kratom Buy Kratom Extract   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Mushroom-Hut Liquid Cultures


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