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Shop: Left Coast Kratom Buy Kratom Extract   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag

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Offlineshoeareyou
Stranger


Registered: 01/07/07
Posts: 204
Last seen: 10 years, 21 days
Woooo!!
    #8144907 - 03/14/08 05:55 AM (16 years, 10 months ago)

Waaaay, Goodies arrived today!

4 x Saboroud Dextrose Agar Slants
1 x Bottle Saboroud dextrose agar
20 x sterile plastic petri dishes

I have a syringe refridgerating,
What should I do first guys!? :laugh:

(Unfortunately im still waiting on 12.5Kg of rye grain, its taking absoloutely ages.)

however, my other rye grain jars are at 4 days germination and beginning to see tiny signs of life!

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Offlineshevanel
Gone til November
 User Gallery


Registered: 01/27/08
Posts: 1,517
Last seen: 2 years, 11 months
Re: Woooo!! [Re: shoeareyou]
    #8144913 - 03/14/08 06:06 AM (16 years, 10 months ago)

Quote:

What should I do first guys!?





Take your picture down, unless it's a clever way of thowing off the D.

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Invisibleshroober
Myco Junkie


Registered: 01/02/08
Posts: 879
Re: Woooo!! [Re: shevanel]
    #8144938 - 03/14/08 06:31 AM (16 years, 10 months ago)

start isolating, mix up your agar and pour into the petri dishes, noc up a couple with your syringe, then start isolating (obviously you will need to read up on isolating and agar if you've never done it before)

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Offlineshoeareyou
Stranger


Registered: 01/07/07
Posts: 204
Last seen: 10 years, 21 days
Re: Woooo!! [Re: shroober]
    #8149113 - 03/15/08 11:41 AM (16 years, 10 months ago)

Yep, Done. I've read the mushroom cultivator like cover to cover 6 or 7 times now, and am constantly using it for reference.

I've innoculted 5 plates with a drop or two each (should be plenty) using my glovebox with sterile techique. Will post a propper growthread monday - tuesday when i get the pics off my phone.

was also pasteurizing some rye, and unfortunately one of my autoclave bags burst, i think the PC dried out a little and melted it. Nevermind, still got a 1/2 galon bag with self healing filter patch ready to go, its cooling atm.

Also, removed one of my previous rye jars which had wet spot. Pity, because i know exactly when it happened too - The lid just came off it for a second, and I said to myself in my head, yep, thats fucked. And sure enough it was.

The other ones are on day 5 but no sign of germination yet.

*waiting game..*

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Offlineshoeareyou
Stranger


Registered: 01/07/07
Posts: 204
Last seen: 10 years, 21 days
Re: Woooo!! [Re: shoeareyou]
    #8149157 - 03/15/08 11:52 AM (16 years, 10 months ago)

forgot to say, also did the four slants and everything is generally incubating at 30*C in my new aquarium heater style floating incubator.

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