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Offlinecokaznrebel
BBQ CHK CHZ STK

Registered: 11/08/04
Posts: 328
Loc: Mile High
Last seen: 4 years, 4 months
Would this LC method work?
    #8142345 - 03/13/08 05:17 PM (16 years, 10 months ago)

Okay so im going to be trying to make some LC's for my third attempt and would like to know if thier is anything wrong with my procedure.

Im going to be using 3, 1/2 pint ball jars with a nail sized hole poked into the lid.

Ill add 250 ml' of water with 1 tablespoon of karo light to each jar.

Put the lid on, then a tyvek cutout, then the ring.

PC for 15 minutes at 15 PSI

Let cool overnight inside the PC.

Remove jars and bring to a glove-box to inoculate in sterile conditions.

Put micropore tape over inoculation site.

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Offlinedizzy_jester
I aint noclown...

Registered: 03/16/07
Posts: 158
Last seen: 15 years, 10 months
Re: Would this LC method work? [Re: cokaznrebel]
    #8142400 - 03/13/08 05:29 PM (16 years, 10 months ago)

put a marble in the pint jar when you sterilize...it will help for when the LC is done in breaking up the mycellium.

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Offlinecokaznrebel
BBQ CHK CHZ STK

Registered: 11/08/04
Posts: 328
Loc: Mile High
Last seen: 4 years, 4 months
Re: Would this LC method work? [Re: dizzy_jester]
    #8142477 - 03/13/08 05:44 PM (16 years, 10 months ago)

Quote:

dizzy_jester said:
put a marble in the pint jar when you sterilize...it will help for when the LC is done in breaking up the mycellium.




Good addition, i didnt think of that.

Does anybody notice any flaws with my procedure?

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Offlinedizzy_jester
I aint noclown...

Registered: 03/16/07
Posts: 158
Last seen: 15 years, 10 months
Re: Would this LC method work? [Re: cokaznrebel]
    #8142886 - 03/13/08 07:15 PM (16 years, 10 months ago)

i use the silicone on the inoculation hole method.... remember after pc'ing that the jar will create a vacuum. so when you go to inject the spores it will literally suck your syringe. So if you do this all you need is a lil prick. but i noticed with this method you should also get a sterilized syringe to inject AIR in to the jar to help the mycellium grow. some peeps around here dont think its necessary but ive noticed much more growth injecting more air into the jar

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