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Offlineivangrozny
Stranger
Registered: 03/09/08
Posts: 19
Last seen: 11 years, 9 months
Returning to growing after a long break
    #8142323 - 03/13/08 05:11 PM (16 years, 10 months ago)

After 25 years away I want to start growing mushrooms again. Many things have changed and some interesting developments while I was away.

So I was thinking of starting out with PF jars because of the simplicty. After I have my first fruits I would like to get a tissue culture from them on agar, MEA or cornmeal dextrose agar, and then getting it to LC which I am most comfortable working with from past experience. As I get set up and comfortable with the processes again I want to move on to manure, straw and manure straw composts. I got great results in the past useing manure straw composts, and it is a beautiful site to see a big bed in full flush. Does this sound like a decent plan?

Thinking of taking advantage of one vendors special which is 7 syringes for 50 bux. How long will the syringes remain viable in the fridge? Can syringes really be autoclaved and reused?

Edited by ivangrozny (03/14/08 03:29 AM)

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Invisibleshaggydogman
Stranger


Registered: 04/20/07
Posts: 672
Re: Returning to growing adter a long break [Re: ivangrozny]
    #8142420 - 03/13/08 05:32 PM (16 years, 10 months ago)

Sounds like a good plan to me. :smile: Welcome back to the hobby...

I'm not sure how long a syringe would remain viable in the fridge, but once you start getting fruits you can practice spore printing and syringe making as well!! :smile:

I regularly PC syringes and needles and reuse them, although I tend to use less and less syringes and more and more Grain to grain transfers. 1 syringe > 8 grain  masters > g2g transfer to 8 jars from each master... 64 jars from one syringe! Could even take it to the 3rd generation for 512 jars! That spawn just keeps expanding!

Have fun and Good luck... :smile:


--------------------
Rye -- WBS | Grain LC -- G2G | Bulk -- Monotub | 50/50 -- Late Casing -- A Pinning Strategy
Disclaimer: My opinion is subject to change at short notice subject to but not limited by new information and knowledge being made available.

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Offlineivangrozny
Stranger
Registered: 03/09/08
Posts: 19
Last seen: 11 years, 9 months
Re: Returning to growing adter a long break [Re: shaggydogman]
    #8144797 - 03/14/08 03:39 AM (16 years, 10 months ago)

anyone on the question of spore syringe longevity under refrigeration? I doubt I want to do enough jars at 1 time to use up 7 syringes, but the deal is so good and they srea nice strains.

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Invisibleshaggydogman
Stranger


Registered: 04/20/07
Posts: 672
Re: Returning to growing adter a long break [Re: ivangrozny]
    #8144833 - 03/14/08 04:36 AM (16 years, 10 months ago)

I searched for "long +syringes +keep" and it gave me over 800 threads. I read a few and the general consensus seems to be the earlier you use them the better with about a year being the aim to use by period, there are also some reports of 6 year old syringes working. Well made syringes + good storage seem to be the key!

Here's 2 comments from a couple of old hands on the subject.
http://www.shroomery.org/forums/showflat.php/Number/6997742#Post6997742
http://www.shroomery.org/forums/showflat.php/Number/6712826#Post6712826


--------------------
Rye -- WBS | Grain LC -- G2G | Bulk -- Monotub | 50/50 -- Late Casing -- A Pinning Strategy
Disclaimer: My opinion is subject to change at short notice subject to but not limited by new information and knowledge being made available.

Edited by shaggydogman (03/14/08 04:46 AM)

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InvisibleMalkoth
Stalking Butler


Registered: 03/19/01
Posts: 85
Re: Returning to growing adter a long break [Re: shaggydogman]
    #8144878 - 03/14/08 05:14 AM (16 years, 10 months ago)

i cant say for certain how long they remain reliable in the fridge but ive used a couple of syringes that were left in a closet for 1 1/2 years and they still worked.

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Offlineivangrozny
Stranger
Registered: 03/09/08
Posts: 19
Last seen: 11 years, 9 months
Re: Returning to growing adter a long break [Re: Malkoth]
    #8145313 - 03/14/08 09:40 AM (16 years, 10 months ago)

Wow, thanks guys.
that will do just fine, I was really planning on useing them all within a 2-4 month period of time so it seems I am well within the safe zone.
Thanks for the search Shaggy, I guess I could have done that if I wasn't brain dead this morning.

So I guess it is time to rock on and take the plunge.

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