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Invisibleshroober
Myco Junkie


Registered: 01/02/08
Posts: 879
Re: my LCs germinated quick and strong and have now slowed down? [Re: JSshroom]
    #8136155 - 03/12/08 10:19 AM (15 years, 11 months ago)

Quote:

JSshroom said:
yes they will look different.  cobweb, the green and all the other fun colors will come out on a substrate :eek:




it will look different when inoculated into a substrate but not necessarily the LC itself. it is very possible for the LC to look normal but be loaded with contams. This is one of the inherent weaknesses to LC. If you are not sure about the LC you can always noc up a half pint jar to see if it colonizes with healthy myc, thats typically a sure-fire way to detect if there are any contams within your LC

But again, if you noc up your LC with healthy mycelium as opposed to spores you can be much more confident that your LC will be successful assuming you followed proper sterile procedures.


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Offlinedill705
Amazed
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Registered: 12/10/07
Posts: 3,779
Loc: The Cat's Cradle
Last seen: 6 years, 4 months
Re: my LCs germinated quick and strong and have now slowed down? [Re: shroober]
    #8136221 - 03/12/08 10:35 AM (15 years, 11 months ago)

Why should it matter if you inoculate an LC with spores?

It's no different then inoculating PF jars with spores. The contams would get there the same way anyways.

If your vendor is selling you spore syringes with contams IN them, I'd switch to Sporeworks!


--------------------
My advice is to find those things that give pleasure and do them often without too much attachment and relax and wait for the show to end.

-Icelander-

I like free markets and all. Truly I do, at least in general, but there needs to be some kind of oversight in recognition of sustainability. Life works the same way, on a bunch of sustainable systems. Why not honor what made us what we are and take some lessons? Nature FTW!

~dill705~


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Invisiblejeetered
Stranger
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Registered: 07/07/06
Posts: 3,055
Loc: no clue
Re: my LCs germinated quick and strong and have now slowed down? [Re: dill705]
    #8136228 - 03/12/08 10:37 AM (15 years, 11 months ago)

or ralphstersspores


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Invisibleshroober
Myco Junkie


Registered: 01/02/08
Posts: 879
Re: my LCs germinated quick and strong and have now slowed down? [Re: dill705]
    #8136242 - 03/12/08 10:42 AM (15 years, 11 months ago)

Quote:

dill705 said:
Why should it matter if you inoculate an LC with spores?

It's no different then inoculating PF jars with spores. The contams would get there the same way anyways.

If your vendor is selling you spore syringes with contams IN them, I'd switch to Sporeworks!




not everyone uses properly prepared spores, many people do trades and unfortunately sometimes you get some spores that have been contaminated. its not a problem if you use agar and are able to extract the healthy myc from the contam.

With a PF jar you can see contams pretty quickly. With an LC it will take much longer to notice contams and often times contams can go totally undetected in LC. Also, if you noc up a PF jar and it gets contamed you can simply throw away the jar and use the other healthy jars. With LC typically you don't make more than a couple of jars. I would much rather lose a PF jar to contams than an entire LC jar that has been colonizing for days or weeks.

Thats not to say you CANT noc up LC with spores, I've done it and it worked out fine. Its just safer IMO to noc up LC with mycelium so you can be more certain that your LC will be healthy.

And i agree, sporeworks kicks ass!


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Offlinedill705
Amazed
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Registered: 12/10/07
Posts: 3,779
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Re: my LCs germinated quick and strong and have now slowed down? [Re: shroober]
    #8136262 - 03/12/08 10:46 AM (15 years, 11 months ago)

5 shrooms for sporeworks, that's for sure.

I knew you'd come back with the "home print" thing. What can I say, but this guy I believe (or it was a different thread) already stated that he got his spores from Ralphsters, so his LC should be good. I'm ordering some Pan Cyan unless I can get a trade, and I'm nocing up LC directly for more genetic variation.

I hope we hear back about this "contam" issue soon...


--------------------
My advice is to find those things that give pleasure and do them often without too much attachment and relax and wait for the show to end.

-Icelander-

I like free markets and all. Truly I do, at least in general, but there needs to be some kind of oversight in recognition of sustainability. Life works the same way, on a bunch of sustainable systems. Why not honor what made us what we are and take some lessons? Nature FTW!

~dill705~


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Invisibleshroober
Myco Junkie


Registered: 01/02/08
Posts: 879
Re: my LCs germinated quick and strong and have now slowed down? [Re: dill705]
    #8136310 - 03/12/08 10:57 AM (15 years, 11 months ago)

Quote:

dill705 said:
5 shrooms for sporeworks, that's for sure.

I knew you'd come back with the "home print" thing. What can I say, but this guy I believe (or it was a different thread) already stated that he got his spores from Ralphsters, so his LC should be good. I'm ordering some Pan Cyan unless I can get a trade, and I'm nocing up LC directly for more genetic variation.

I hope we hear back about this "contam" issue soon...




I agree, if i got spores directly from Ralphsters or Sporeworks I would feel much more comfortable inoculating an LC directly


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Offlinejonogt
Jon
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Registered: 12/27/07
Posts: 104
Last seen: 15 years, 8 months
Re: my LCs germinated quick and strong and have now slowed down? [Re: shroober]
    #8137234 - 03/12/08 02:37 PM (15 years, 11 months ago)

yup Cambodians from Ralph. My little test jar is for the LC in the pic, but I determined that the 2nd one was unfit for use, either by contams or damage or what have you (beige ish discolorations in the whisps were getting darker). I made a new one with slightly higher conc. of turbinado sugar (~2.8%) in a larger volume jar. Coffee filtered the sugar solution prior to PCing ~35 minutes at ~15psi to keep things clearer to see what's going on.

Sealed my room off from all air exchange, gave all the walls and ceiling a once over with lysol, hotboxed the place with my ionic breeze, and oust bombed from multiple areas. Let the jar come to 75F, went and sat down at the desk very still a few minutes, oust bombed, sat still again, oust again. Glazed my desk, the syringe, the jar, and misted my walls/ceiling (on the desk's side of the room), skullcap (with all my hair tight up in it), bandana (tied over mouth and nose), neck, whole torso, hands and arms up to the shoulders with rubbing alcohol. Sat perfectly still with the oust in my hand for 10 minutes, and with only moving my arm, dispensed it up over the desk until it was a wide thick haze. Once it started raining down, I popped the sheath off the needle under an alcy soaked paper towel, gave the whole syringe and needle a good whiping with it. Went straight to the small alcohol candle on the desk and brought the tip to a dull red. I think some spores in it got burned because they formed a crusty obstructive barrier that made squirting a few drops otu quite laborious. I was able to pick this stuff out with my fingernail through the alcy soaked paper towel pretty easily, and then I reheated it again, squirted a few drops to clear it, and quenched in the paper towel with no problem. I held it in the paper towel by the tip while I swabbed my injection port clean. Then I sorta formed the paper towel with the needle in it into a tent of the rubbing alcohol's vapors over the injection port and inoculated it. Pulled the needle through the pinched paper towel while withdrawing it, and formed the tent again to replace the sheath.

I think the extent I took things to mmmight have been borderline manic and just excessive, but I want to feel confident that if any contams show up, I'll be able to rule out the procedural sterility variables and investigate from there.

This one only got a fraction of the spores that the first one did... no massive chunks. so it probably won't spring into action like before. But w/e I'll wait n see

-Jon


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Offlinedill705
Amazed
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Re: my LCs germinated quick and strong and have now slowed down? [Re: jonogt]
    #8137251 - 03/12/08 02:41 PM (15 years, 11 months ago)

How about the jar your growing out?

Anything suspicious?


--------------------
My advice is to find those things that give pleasure and do them often without too much attachment and relax and wait for the show to end.

-Icelander-

I like free markets and all. Truly I do, at least in general, but there needs to be some kind of oversight in recognition of sustainability. Life works the same way, on a bunch of sustainable systems. Why not honor what made us what we are and take some lessons? Nature FTW!

~dill705~


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Offlinejonogt
Jon
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Registered: 12/27/07
Posts: 104
Last seen: 15 years, 8 months
Re: my LCs germinated quick and strong and have now slowed down? [Re: dill705]
    #8138825 - 03/12/08 08:24 PM (15 years, 11 months ago)

I see a red-ish dot near the edge of the jar, accompanied with 3 or so specs elsewhere in the paste that are something between red and brown (to small to tell really well) It looks like how a speck of rust looks that gets smeared out by cloth or moving liquid. It's about 1/2mm across so I'm keeping an eye on it to see if it grows.
Any guess what it might be? Are there any white/clear contams that grow red-ish once in a substrate? Pretty much everything went wrong from the start with this little thing, and I just didn't redo it immediately because if it came back clean with how shitty it was made, I could be sure it was good. Has anyone observed a contam like this come FROM THE LC? Or would you guys hypothesize that it came in during the sloppy inoculation?
If there are no known contams that can hide in an LC that turn red like this, I'll certainly retest it and try to clear this jar's name (-:


The paste is aweful light in color for "brown" rice and while I think I see some white on the surface, it's very hard to tell. I can't determine what's myc and what's light reflections.

I looked Agar's thread up again (what inspired me to use turbinado) and his pics make me feel a little better about how my culture grew.
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/6378527/page/0/fpart/1/vc/1
(bout 3/4 the way down)

I've started occasionally mixing it with short high speed bursts on the magnetic stirrer, taking it up to speeds where the vortex is barely even cone shaped and it saturates the liquid with tiny air bubbles. It seems to help break the myc into more ropey pieces instead of just cohesive balls, and I'm fairly certain it's grown a bit more as a result. Right after I take it off the stirrer, it looks almost as heavy with myc pieces as agar's pic.

There are other people in that thread that say their turbinado LCs are stopping with a curiously small amt of myc in them. While I'll still be retesting mine for contam, surely all these people can't have them. Is just turbinado sugar to inbalanced of a diet for the myc to continue showing rigorous growth? monstermitch's recipe for example uses 3 or 4 (i beleive?) different ingredients. Surely this variety of complex and simple sugars is beneficial, no?


Edited by jonogt (03/12/08 09:51 PM)


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Offlinejonogt
Jon
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Registered: 12/27/07
Posts: 104
Last seen: 15 years, 8 months
Re: my LCs germinated quick and strong and have now slowed down? [Re: jonogt]
    #8139970 - 03/13/08 12:11 AM (15 years, 11 months ago)

I don't have a coffee grinder to get rice very well powdered and I tried to use a blender and it worked alright but still left chunks and the rice is just to light to see myc very well and this BRF paste thing is just a mess and really not my cup of tea.

Would there be any harm (beyond the obvious loss of time/materials) in skipping that and making a few half pint grain jars to inoculate with my LCs as a test? That way if it's good, I already have spawn on its way. If it's not, I'm only out a few ounces of rye berries. Wouldn't this also be a good way to test different levels of moisture? Make each one with a tad bit more or less and see which one the myc takes to best?

If it seems like I'm dragging my feet and making everything more difficult than it should be etc, I can't really help it. My current location (college town) doesn't have jack shit as far as ingredients and proper anything to do ANY of this stuff. Spring break starts friday and when I'm home I'll be obtaining good supplies of organic rye berries, light dry malt, dextrose, my hydrofarm heat mat, some hypoderm needles that aren't designed to puncture the great wall of china, and w/e else I find I need while there.

Unless someone has some objection to me making these half pint grain jars, I'm gonna do those while home too. If properly sterilized and kept with tin foil over the top, are grains pretty safe to leave un-inoculated for a couple days? Should they be kept cool or room temp or what? Situation being there might be a time gap between when we make em and when I get back up to school, cause I'm not gonna try and take my LCs with me driving halfway across the state, so I can't inoculate till I get back.



sorry that this has gotten a bit off topic
thanks for the help
-Jon


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