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Shop: North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures

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InvisibleRahz
Alive Again
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Registered: 11/10/05
Posts: 10,314
Tyvek lids and syringes
    #8138930 - 03/12/08 08:40 PM (16 years, 10 months ago)

This is just how I do it, might help someone out. Feel free to add ideas, variations, etc.

I cut the tyvek up into 4.75" squares. These will be used with wide mouth jars, much recommended. After loading the jars, I put the lid on, then place tyvek over the lid, and secure with a rim.

After the jars have cooled from cooking, I place a small dollop of high-temp RTV sealant on each of the tyvek covers for use as a self sealing inoculation port. These can be tapped flat using a soaked piece of wood (clothes pin or popsicle stick). I've tried other sealants and still use the RTV, even though I apply the sealant after cooking. It resists being cut as it's punctured, so nickle thin will do the job, though mine always end up a little thicker.

I'm curious to know if there are other suitable sealants. I've tried others, but had a few puncture wounds I didn't like.

I normally use 3/8" holes because I ruined a few needles trying to judge the center of the tyvek cover. If I wanted to use 1/4" holes, I would use an extra lid for perfect aim while inoculating. I'm lazy though, so I stick with 3/8" holes. Might be good in drier areas.

The reason I put the tyvek on top and use a sealant port? I work in non-sterile conditions, so it cuts the possibility of an open air contam getting in. At no point is there an entry way into the jar, except through or on the needle, and I would guess that the sealant would be somewhat effective at catching contams as the needle penetrated. At least better than just tyvek. Along with some alcohol, a clean syringe, and a flame, it works out to be very safe.

I've played with different ways of sterilizing needles including filling the syringe with alcohol, letting it sit, and rinsing with boiling water before use; placing the syringe in a jar and PCing it, which is better to me than wrapping in a piece of foil, same difference though. I've also boiled the needle while microwaving the syringe, but I'm more keen on keeping the needle attached the whole time. How do you guys sterilize syringes?


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rahz

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