|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
flavoraid
now with twicethe ketamine andopiates!

Registered: 12/05/07
Posts: 1,678
|
Frozen Liquid Mycelium Culture
#8121758 - 03/08/08 11:14 PM (16 years, 10 months ago) |
|
|
Is it viable after being thawed?
Speaking of cubensis not shiitake or other edibles.
My lovely assistant P.Menace is going to test a LC of penis envy freezing it then thawing it and trying to use it as inoculant.
Feedback encouraged.
-------------------- coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.
|
81Renaissance
Mad Scientist



Registered: 11/01/06
Posts: 457
Last seen: 10 months, 28 days
|
Re: Frozen Liquid Mycelium Culture [Re: flavoraid]
#8121768 - 03/08/08 11:16 PM (16 years, 10 months ago) |
|
|
I think a hard freeze would kill mycelium just like intense heat does...but I'm no expert, so let him without ignorance cast the first stone.
-------------------- "Every passing moment is another chance to turn it all around."
|
flavoraid
now with twicethe ketamine andopiates!

Registered: 12/05/07
Posts: 1,678
|
Re: Frozen Liquid Mycelium Culture [Re: 81Renaissance]
#8121774 - 03/08/08 11:17 PM (16 years, 10 months ago) |
|
|
P.Menace is going to freeze some LC at 3pm tomorrow.
-------------------- coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.
|
P.Menace
Contradicting the Status Quo




Registered: 11/17/07
Posts: 4,797
Loc: Let Me See Potato Salad!!...
Last seen: 10 years, 10 months
|
Re: Frozen Liquid Mycelium Culture [Re: 81Renaissance]
#8121776 - 03/08/08 11:19 PM (16 years, 10 months ago) |
|
|
Im not at the grow place now so i cant throw it in until tomorrow at 3 pm... now i have personally thrown a casing in the fridge in water for a dunk and froze that bitch solid for a few days accidentally... but it fruits today, no shit
--------------------
http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace
roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
|
flavoraid
now with twicethe ketamine andopiates!

Registered: 12/05/07
Posts: 1,678
|
Re: Frozen Liquid Mycelium Culture [Re: P.Menace]
#8121779 - 03/08/08 11:19 PM (16 years, 10 months ago) |
|
|
Quote:
RogerRabbit said: If it doesn't survive, try it next time after putting a popsicle stick or tongue depressor, etc. in with the LC when you make it. The myc will begin to colonize the wood, and I believe that might help it survive. It's how oyster mushrooms in dead trees survive the winter. I use a piece of wood in all my test tube slants, and store them at 35F/+2C. RR
-------------------- coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.
|
81Renaissance
Mad Scientist



Registered: 11/01/06
Posts: 457
Last seen: 10 months, 28 days
|
Re: Frozen Liquid Mycelium Culture [Re: flavoraid]
#8121791 - 03/08/08 11:22 PM (16 years, 10 months ago) |
|
|
Now THAT is pretty badass. and the reason why I didn't make a declaratory statement.
-------------------- "Every passing moment is another chance to turn it all around."
|
P.Menace
Contradicting the Status Quo




Registered: 11/17/07
Posts: 4,797
Loc: Let Me See Potato Salad!!...
Last seen: 10 years, 10 months
|
Re: Frozen Liquid Mycelium Culture [Re: 81Renaissance]
#8123833 - 03/09/08 04:16 PM (16 years, 10 months ago) |
|
|
at 3pm i placed a syringe full of PE LC in the Freezer... we decided it best to freeze it for 48-72 hours, so it shall be done... probably 72, as Flavoraid may forget it in his mail like he did some spore syringes once... Then i will get some supplies and make some PF cakes and inoculate them with the previously frozen, now thawed PE LC.... What do you think will happen?
--------------------
http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace
roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
|
flavoraid
now with twicethe ketamine andopiates!

Registered: 12/05/07
Posts: 1,678
|
Re: Frozen Liquid Mycelium Culture [Re: P.Menace]
#8124566 - 03/09/08 07:01 PM (16 years, 10 months ago) |
|
|
if you don't test it like this im going to.
2 lcs... one with wood in it another without
samples taken from lcs and frozen for 24 and 72 hours then thawed completely and used for an inoculant.
that would be a pretty scientific test.
-------------------- coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.
|
P.Menace
Contradicting the Status Quo




Registered: 11/17/07
Posts: 4,797
Loc: Let Me See Potato Salad!!...
Last seen: 10 years, 10 months
|
Re: Frozen Liquid Mycelium Culture [Re: flavoraid]
#8124579 - 03/09/08 07:02 PM (16 years, 10 months ago) |
|
|
so i would have to make another one and it would take another day but ok i didnt think of that... i will do it that way
--------------------
http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace
roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
|
flavoraid
now with twicethe ketamine andopiates!

Registered: 12/05/07
Posts: 1,678
|
Re: Frozen Liquid Mycelium Culture [Re: P.Menace]
#8124585 - 03/09/08 07:03 PM (16 years, 10 months ago) |
|
|
mushroom dorkage
-------------------- coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.
|
smokedout420
The EnlightenedOne



Registered: 12/16/07
Posts: 484
|
Re: Frozen Liquid Mycelium Culture [Re: flavoraid]
#8124927 - 03/09/08 08:17 PM (16 years, 10 months ago) |
|
|
idk about lc, but on another site they tried spores and the out come was bad.
good luck
|
P.Menace
Contradicting the Status Quo




Registered: 11/17/07
Posts: 4,797
Loc: Let Me See Potato Salad!!...
Last seen: 10 years, 10 months
|
Re: Frozen Liquid Mycelium Culture [Re: smokedout420]
#8124958 - 03/09/08 08:23 PM (16 years, 10 months ago) |
|
|
flav froze spores and his were fine
--------------------
http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace
roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
|
flavoraid
now with twicethe ketamine andopiates!

Registered: 12/05/07
Posts: 1,678
|
Re: Frozen Liquid Mycelium Culture [Re: P.Menace]
#8124966 - 03/09/08 08:24 PM (16 years, 10 months ago) |
|
|
the problem with frozen syringes is thawing too fast. the syringes crack and shit.
spores survive freezing for some reason I used to be under the impression that they burst or some shit.
-------------------- coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.
|
flavoraid
now with twicethe ketamine andopiates!

Registered: 12/05/07
Posts: 1,678
|
Re: Frozen Liquid Mycelium Culture [Re: flavoraid]
#8137563 - 03/12/08 04:04 PM (16 years, 10 months ago) |
|
|
the following experiments will be made
freezing of faht style corn jar. basically seeing if you can pull liquid culture from a prefrozen colonized grain.
freezing of a lc w/o wood in it
freezing of a lc with colonized wood in it
-------------------- coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.
|
|