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Offlineusr
Interested.
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Registered: 09/16/07
Posts: 164
Loc: United States
Last seen: 8 months, 24 days
I've got a serious contamination problem...
    #8107263 - 03/05/08 05:49 PM (16 years, 17 days ago)

...and for the life of me, I dont know why.

Obviously is boils down to the fact that Im doing something wrong, but Im driving myself nuts trying to figure it out.

My first grow ever, around 6 months ago, I inoculated 24 half-pint jars and got multiple flushes from 20 of those.

Second attempt, I inoculated 20 jars and all but around 6 contaminated during colonization. I owned up to this one, as I felt I was a bit sloppy with my sterilization procedures.

Third, and most recent, attempt. I lost all 20 half-pint jars. I inoculated these jars around 3 weeks ago and I was seeing a good bit of mycelium growth. The thing is, these jars all show contamination later and later. As in when I checked after a week, around five had mold. Another few days, a few more, another few days, a few more, etc.

I used the exact same procedures as I did the very first time I ever attempted a grow, in the same location. It has to be something Im doing during inoculation, I guess. I cook all jars for even longer than required, just to be sure. I flamed the needle between each jar, use lysol between every few jars, wiped everything down with alcohol before ever starting, and during, shooting up the jars.

I know Im not doing something right, but what? Why did this procedure work the first time with 20 jars, and the time Im extra careful, I lose all my jars?

Confusing.

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Invisibleim_on_a_boat
Stranger

Registered: 04/06/06
Posts: 3,950
Re: I've got a serious contamination problem... [Re: usr]
    #8107302 - 03/05/08 05:56 PM (16 years, 17 days ago)

i dunno man i've been having a really tough time lately.. every grain jar is falling to cobweb..

could be your syringe or whatever you're using to inoc..

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OfflineJusListen
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Registered: 12/18/07
Posts: 403
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Re: I've got a serious contamination problem... [Re: usr]
    #8107312 - 03/05/08 05:57 PM (16 years, 17 days ago)

did you clean out wherever you incubate?

I had this same prob one time,
but, once I cleaned the TiT, I seen thats what the prob was.

I'd suggest cleaning most everything, not just the jars,
clean wherever you incubate, and also your FC, every grow.

just my 2 cents.

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Offlineusr
Interested.
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Registered: 09/16/07
Posts: 164
Loc: United States
Last seen: 8 months, 24 days
Re: I've got a serious contamination problem... [Re: JusListen]
    #8107788 - 03/05/08 07:34 PM (16 years, 17 days ago)

I actually didn't clean the incubation tubs this last time...

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OfflineThe shroomy 1
Luminous beings surround me
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Registered: 03/27/07
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Re: I've got a serious contamination problem... [Re: usr]
    #8108716 - 03/05/08 10:00 PM (16 years, 17 days ago)

In the future, ALWAYS, AND I DO MEAN ALWAYS, have a "control jar" If you have 20 jars prepped, only inoculate 19 and have the extra jar labeled "control". I've been preaching this for a while now. Do this with casing material too. In my opinion, it is invaluable. If a couple of jars go bad, and the "control jar" is contaminant free, then it eliminates half the possibilities of where you went wrong. Example. You had 20 jars go bad.... If your control jar was contaminant free, you could make a case for a bad syringe. Get my drift? I'm sorry for your loss, and I hope that you follow the advice above on your next grow.

The Shroomy 1


--------------------


AMU Q&A thread.

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Offlineusr
Interested.
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Registered: 09/16/07
Posts: 164
Loc: United States
Last seen: 8 months, 24 days
Re: I've got a serious contamination problem... [Re: The shroomy 1]
    #8110004 - 03/06/08 07:17 AM (16 years, 16 days ago)

Makes sense.

I'm not sure why it never crossed my mind. Maybe I was too confident in my first grow and got sloppy.

Going to try again this weekend. I'll sanitize and clean absolutely everything I possibly can.

I appreciate all the input!

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Invisiblelarge_dose
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Registered: 05/05/06
Posts: 1,346
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Re: I've got a serious contamination problem... [Re: usr]
    #8111713 - 03/06/08 03:53 PM (16 years, 16 days ago)

Quote:

Maybe I was too confident in my first grow and got sloppy.




We all have a failure, after a success...
for the exact reason stated above.

Quote:

Going to try again this weekend




Try working in the back of a trashbag, or in a closet with the air full of oust. The more you can keep the air from moving around, the better.

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Offlinefiasco
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Registered: 02/10/08
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Loc: sc/nyc
Last seen: 15 years, 27 days
Re: I've got a serious contamination problem... [Re: The shroomy 1]
    #8114436 - 03/07/08 12:56 AM (16 years, 15 days ago)

Quote:

The shroomy 1 said:
In the future, ALWAYS, AND I DO MEAN ALWAYS, have a "control jar"  If you have 20 jars prepped, only inoculate 19 and have the extra jar labeled "control".  I've been preaching this for a while now.  Do this with casing material too.  In my opinion, it is invaluable.  If a couple of jars go bad, and the "control jar" is contaminant free, then it eliminates half the possibilities of where you went wrong.  Example.  You had 20 jars go bad.... If your control jar was contaminant free, you could make a case for a bad syringe.  Get my drift?  I'm sorry for your loss, and I hope that you follow the advice above on your next grow.

The Shroomy 1




good idea:thumbup:


--------------------

Fiasco's Magic Quesdilla
///All posts are merely the result of a delusional mind.\\\

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Offlineusr
Interested.
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Registered: 09/16/07
Posts: 164
Loc: United States
Last seen: 8 months, 24 days
Re: I've got a serious contamination problem... [Re: fiasco]
    #8124705 - 03/09/08 07:26 PM (16 years, 13 days ago)

So as I type this, I have around 20 contaminated jars in the PC. I plan on leaving them in there for a good hour and a half, letting them cool overnight, then emptying the contents tomorrow and tossing them in the dishwasher before preparing them and tossing them in the PC tomorrow.

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