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Invisibleflavoraid
now with twicethe ketamine andopiates!
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Registered: 12/05/07
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Frozen Liquid Mycelium Culture
    #8121758 - 03/08/08 11:14 PM (16 years, 10 months ago)

Is it viable after being thawed?

Speaking of cubensis not shiitake or other edibles.

My lovely assistant P.Menace is going to test a LC of penis envy freezing it then thawing it and trying to use it as inoculant.

Feedback encouraged.


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coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.

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Offline81Renaissance
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Re: Frozen Liquid Mycelium Culture [Re: flavoraid]
    #8121768 - 03/08/08 11:16 PM (16 years, 10 months ago)

I think a hard freeze would kill mycelium just like intense heat does...but I'm no expert, so let him without ignorance cast the first stone.


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"Every passing moment is another chance to turn it all around."

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Invisibleflavoraid
now with twicethe ketamine andopiates!
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Re: Frozen Liquid Mycelium Culture [Re: 81Renaissance]
    #8121774 - 03/08/08 11:17 PM (16 years, 10 months ago)

P.Menace is going to freeze some LC at 3pm tomorrow.


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coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.

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OfflineP.Menace
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Re: Frozen Liquid Mycelium Culture [Re: 81Renaissance]
    #8121776 - 03/08/08 11:19 PM (16 years, 10 months ago)

Im not at the grow place now so i cant throw it in until tomorrow at 3 pm... now i have personally thrown a casing in the fridge in water for a dunk and froze that bitch solid for a few days accidentally... but it fruits today, no shit


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http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace


roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.

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Invisibleflavoraid
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Re: Frozen Liquid Mycelium Culture [Re: P.Menace]
    #8121779 - 03/08/08 11:19 PM (16 years, 10 months ago)

Quote:

RogerRabbit said:
If it doesn't survive, try it next time after putting a popsicle stick or tongue depressor, etc. in with the LC when you make it. The myc will begin to colonize the wood, and I believe that might help it survive. It's how oyster mushrooms in dead trees survive the winter. I use a piece of wood in all my test tube slants, and store them at 35F/+2C.
RR




--------------------
coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.

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Offline81Renaissance
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Re: Frozen Liquid Mycelium Culture [Re: flavoraid]
    #8121791 - 03/08/08 11:22 PM (16 years, 10 months ago)

Now THAT is pretty badass. and the reason why I didn't make a declaratory statement.


--------------------
"Every passing moment is another chance to turn it all around."

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OfflineP.Menace
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Re: Frozen Liquid Mycelium Culture [Re: 81Renaissance]
    #8123833 - 03/09/08 04:16 PM (16 years, 10 months ago)

at 3pm i placed a syringe full of PE LC in the Freezer... we decided it best to freeze it for 48-72 hours, so it shall be done... probably 72, as Flavoraid may forget it in his mail like he did some spore syringes once... Then i will get some supplies and make some PF cakes and inoculate them with the previously frozen, now thawed PE LC.... What do you think will happen?


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http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace


roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.

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Invisibleflavoraid
now with twicethe ketamine andopiates!
Male
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Posts: 1,678
Re: Frozen Liquid Mycelium Culture [Re: P.Menace]
    #8124566 - 03/09/08 07:01 PM (16 years, 10 months ago)

if you don't test it like this im going to.

2 lcs... one with wood in it another without

samples taken from lcs and frozen for 24 and 72 hours then thawed completely and used for an inoculant.

that would be a pretty scientific test.


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coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.

Extras: Filter Print Post Top
OfflineP.Menace
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Re: Frozen Liquid Mycelium Culture [Re: flavoraid]
    #8124579 - 03/09/08 07:02 PM (16 years, 10 months ago)

so i would have to make another one and it would take another day but ok i didnt think of that... i will do it that way


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http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace


roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.

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Invisibleflavoraid
now with twicethe ketamine andopiates!
Male
Registered: 12/05/07
Posts: 1,678
Re: Frozen Liquid Mycelium Culture [Re: P.Menace]
    #8124585 - 03/09/08 07:03 PM (16 years, 10 months ago)

mushroom dorkage


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coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.

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Invisiblesmokedout420
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Registered: 12/16/07
Posts: 484
Re: Frozen Liquid Mycelium Culture [Re: flavoraid]
    #8124927 - 03/09/08 08:17 PM (16 years, 10 months ago)

idk about lc, but on another site they tried spores and the out come was bad.

good luck

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OfflineP.Menace
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Re: Frozen Liquid Mycelium Culture [Re: smokedout420]
    #8124958 - 03/09/08 08:23 PM (16 years, 10 months ago)

flav froze spores and his were fine


--------------------

http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace


roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.

Extras: Filter Print Post Top
Invisibleflavoraid
now with twicethe ketamine andopiates!
Male
Registered: 12/05/07
Posts: 1,678
Re: Frozen Liquid Mycelium Culture [Re: P.Menace]
    #8124966 - 03/09/08 08:24 PM (16 years, 10 months ago)

the problem with frozen syringes is thawing too fast. the syringes crack and shit.

spores survive freezing for some reason I used to be under the impression that they burst or some shit.


--------------------
coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.

Extras: Filter Print Post Top
Invisibleflavoraid
now with twicethe ketamine andopiates!
Male
Registered: 12/05/07
Posts: 1,678
Re: Frozen Liquid Mycelium Culture [Re: flavoraid]
    #8137563 - 03/12/08 04:04 PM (16 years, 10 months ago)

the following experiments will be made

freezing of faht style corn jar. basically seeing if you can pull liquid culture from a prefrozen colonized grain.

freezing of a lc w/o wood in it

freezing of a lc with colonized wood in it


--------------------
coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.

Extras: Filter Print Post Top
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