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ismokeweed
Stranger


Registered: 11/19/05
Posts: 286
Last seen: 14 years, 10 months
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heres how i did my lc that will hopefully work after lots of tries. what do you think?
#8102261 - 03/04/08 05:06 PM (15 years, 11 months ago) |
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I used a jar I had used for cakes already so it had four holes in the top. (1/2 pint wide mouth) I used about 4% honey. I know too much honey can make for no growth at all but it may have been as little as 2% honey. (any suggestions on a way to know for sure without having a scale?) put the honey in first (just a very small amount) then filled up the jar with tap water.
covered the holes with my fingers and shook it up really well.
then, I covered the top with foil (just like steam sterilizing pf jars) and boiled it for about 30 minutes. no less but maybe up to ten minutes longer. there was a tiny amount of honey sediment after boiling but not much at all.
I found the mushroom I liked best for cloning and picked it. I wiped the whole stem down, a razor, the foil on top of the jar (took if off and then wiped the top of the jar) with isopropyl alcohol.
then I cut open the stem, near the base. cut out a small chunk from the inside and worked it into the biggest hole in the top of the jar, using only the razor (never my hands).
last, rubbed some isopropyl alcohol on the foil again and put it back on top of the jar. I put it in my TiT incubator, which is at 81 degrees) and dark.
It's been 3 days and I see no growth at all. Not even the slightest bit cloudy. I'm pretty sure it's too early to really be worried but I've fucked up 6 before this so I'm getting pretty frustrated with myself. this is the only thing i've had trouble with (though I haven't done anything except 2 successful pf tek grows.)
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bryanbzl
Spawn Runner



Registered: 03/11/07
Posts: 563
Last seen: 5 years, 10 months
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Re: heres how i did my lc that will hopefully work after lots of tries. what do you think? [Re: ismokeweed]
#8102456 - 03/04/08 05:46 PM (15 years, 11 months ago) |
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Sooner than later, everyone is going to switch to honey for the purpose of sediment. Believe it or not, i have never seen any of my karo LC's colonize as fast as my sediment infested honey LC jars. Mycelium seems to love starting its growth from the sediment before moving onto the actual solution. My latest honey LC is a prime example.  Less honey is always better than more anyways. I've used as little as 1% honey and had great success. I'm pretty sure I'm not the only on that notices that as the mycelium grows in the LC the solution gets clearer and clearer. I use about between a 1%-2% honey LC solution and when fully colonized, i get completely clear LC's. If you are really interested you can PM me and ill take some pics that show both the mycelium eating the sediment and the crystal clear jars. Good luck with your LC 
-------------------- Cheers, bzl -------------------------------------------------------------------------------------------------------------------------------------------------------------------------- "From 1898 through to 1910 heroin was marketed as a non-addictive morphine substitute and cough medicine for children." conclusion: poor fucking children of the early 1900's.
Edited by bryanbzl (03/04/08 05:47 PM)
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Civ
Pinning



Registered: 10/14/04
Posts: 2,537
Loc: California
Last seen: 7 months, 5 days
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Re: heres how i did my lc that will hopefully work after lots of tries. what do you think? [Re: ismokeweed]
#8102463 - 03/04/08 05:46 PM (15 years, 11 months ago) |
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That works. You can also take your mushroom and put it in a jar and shake it up with some h202/h20 solution then use the water from the jar to hit up your LC.
Cramming the tissue into the small holes might bruise it up a bit, but it shouldn 't be a problem if it wasn;t.
it can take up too two weeks to see growth. Most the time the myc is in a shock state and wont do anything for a few days anyways.
To messure your honey, just do 2:100 ratio so 2ml honey for each 100 ml of water, that should keep you at 2%.
Quote:
covered the holes with my fingers and shook it up really well.
The oils on your skin can are pretty acidic, and help along with brusing- use some gloves next time or a plastic baggie. GL
-------------------- "...Gal's seem to hate the thought of blending chicken shit in a blender. So, wash it well afterwards & DON'T tell them..." -Agar
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ismokeweed
Stranger


Registered: 11/19/05
Posts: 286
Last seen: 14 years, 10 months
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Re: heres how i did my lc that will hopefully work after lots of tries. what do you think? [Re: Civ]
#8102771 - 03/04/08 06:49 PM (15 years, 11 months ago) |
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alright civ. so you sayin stick the entire mushroom jar and shake it in the solution? what ratio of h202 to h20?
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Civ
Pinning



Registered: 10/14/04
Posts: 2,537
Loc: California
Last seen: 7 months, 5 days
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Re: heres how i did my lc that will hopefully work after lots of tries. what do you think? [Re: ismokeweed]
#8102790 - 03/04/08 06:55 PM (15 years, 11 months ago) |
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Its just another way- not a better way, and not the whole mushroom, a core peice.
you can also use your jars before you spawn with them- I belive its better than useing mushroom matter for sure. Fill up your colonized jar with some water and shake it up- draw out the water with a syringe, use it to hit up some LC.
But, yes, the first one works- but, when I do it now, I use a blender and some h202/h20 and make a slurry.
Store bough h202, use 1:1 with water. Some antibacterial soap (a drop or two) and help fight off some baddies.
-------------------- "...Gal's seem to hate the thought of blending chicken shit in a blender. So, wash it well afterwards & DON'T tell them..." -Agar
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