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Killa_J
Killa

Registered: 05/12/02
Posts: 120
Loc: over here
Last seen: 15 years, 3 months

sterile technique

#809884 - 08/10/02 08:37 PM (22 years, 1 month ago)

No, this in not a general "Why did my PF cake contaminate after I sneezed on it" type of question. This is a question about why absolute sterility is such an issue in the cultivation of fungi. This past semester, I took a microbiology lab. We did numerous cultures of bacteria and yeast (or "east" as the damn biatch pronounced it). We didn't deal with anything like grain, but we worked with a lot of agar. Our only sterility precautions were:

a) rubber gloves
b) bunsen burner to sterilize wire loop
c) using a 10% bleach solution after we were done

I have not had a very good success rate with cultivating mushrooms. Up until probably the last two tries, at least half of my substrate contaminated. Luckily, I am doing better now. Anyway, why is it that out of all the experiments I did in my lab, only one contaminated, but my jars go to shit very easily? Keep in mind this lab was a room with an air conditioner blowing shit around constantly and 7 classes worth of people growing all sorts of fucking stufft. What is going on? Is there something I am missing here, or are fungi just about the most susceptible fucking thing there is to contamination? Anyway, if these are stupid questions, keep in mind that I just got home from a bar and am still wearing a bit of my shitface.

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I feel sorry for people who don't drink. When they wake up in the morning, that's as good as they're going to feel all day. - Frank Sinatra

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Seuss
Error: divide byzero

Registered: 04/27/01
Posts: 23,480
Loc: Caribbean
Last seen: 23 days, 14 hours

Re: sterile technique

[Re: Killa_J]
#810079 - 08/11/02 01:47 AM (22 years, 1 month ago)

My guess is that your home has a much higher free spore load than the lab you were working in. Things like carpet, drapes help hold spores. In the lab you probably had tile floors and plastic or metal blinds. The kitchen and bathroom in your house can also act like a breeding ground for contamination.

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Just another spore in the wind.

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DinoMyc
Ipsa scientiapotestas est

Registered: 11/13/99
Posts: 1,080
Last seen: 18 years, 3 months

Re: sterile technique

[Re: Killa_J]
#810189 - 08/11/02 05:16 AM (22 years, 1 month ago)

I have never seen a microbiology lab, or any biological lab for that matter, which did not have proper air filtration. The ac units you saw/felt very well may have been filtered air.

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If I made affront, I apologize.
If I made affirmation, I apologize.
I merely came to listen, came to say.

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Anonymous

Re: sterile technique

[Re: Killa_J]
#810480 - 08/11/02 08:07 AM (22 years, 1 month ago)

Maybe the agar has antibiotics or fungicides.

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Mushrooms, Mycology and Psychedelics > Advanced Mycology

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