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OfflineFexor
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Question about Syringe
    #8092188 - 03/01/08 11:41 PM (16 years, 10 months ago)

So a couple of days ago I brought a pack of 10 syringes from the local pharmacy, except they were the wrong ones I wanted, they were 1cc and had the tip on them that is used for shooting up.. well I used them anyways on 12 cakes and two Lcs I reused them a couple of times... do you think it would of worked?? much risk of contam due to using the needle more then once? Thanks.

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Invisibletahoe
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Re: Question about Syringe [Re: Fexor]
    #8092313 - 03/02/08 12:08 AM (16 years, 10 months ago)

so you used a 1cc syringe on twelve cakes?
Did you make the syringes? Were they spore syringes?
So did you use 1 syringe per cake?
Did you sterilze the syringes that you re-used?
Are you as stupid as you sound?


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


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OfflineGrowBuddy
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Re: Question about Syringe [Re: tahoe]
    #8092427 - 03/02/08 12:46 AM (16 years, 10 months ago)

Those are somewhat "sketchy" needles that aren't very resiliant looking. If anything I'd toss up contamination due to the fact they certainly aren't meant for multiple uses, although you may get lucky. Don't worry now though since you already did it!


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OfflineFexor
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Re: Question about Syringe [Re: GrowBuddy]
    #8092584 - 03/02/08 02:40 AM (16 years, 10 months ago)

I used 10, 1cc on 10 cakes and two liquid cultures, the reason so is because I did not want to go back into the pharmacy where I got them from, I flamed the needle tip each time before putting the needle into the spore solution and also before injecting into the cakes.

And no I aint stupid I'm just new to this, so stop trying to insult people over the internet it's very immature of you!

Thanks Growbuddy! well there is 10 of them there so maybe I got lucky with one... hopefully :smile: If not I still have cakes fruiting so plenty more spore prints to come :grin:

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OfflineFahkface
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Re: Question about Syringe [Re: Fexor]
    #8092644 - 03/02/08 03:45 AM (16 years, 10 months ago)

I imagine that 1cc syringes are a pain in the ass to work with, since the syringe making process is THE number one contaminants + it's annoying (in my opinion).

Anyway, one CC per jar is OK and if you've worked cleanly and had a little bit of luck, you have good chances to be successful with it.

Re-using syringes and needles isn't a problem at all.
All syringes you buy from the pharmacy are said to be used one time, but since you're not dealing with a matter of human health, but a maximum of fucked up substrate, there's no real problem with using them multiple times.
In fact I still use syringes that were used to inoculate about 30 different jars, with 10 different strains and species for over a year now.
As soon as I get a contams from one of the syringes I throw them out (one 20cc syringe including the needle costs 40 cents here, so there's no problem with that).

Be sure to PC the syringes after every inoculation process, to sterilize them and to flame the needle after every jar, to prevent cross contamination.

As long as you don't have any contam problems with the syringes, and as long as they work fine, you can use them, as often as you want.

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InvisibleBlutjager
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Re: Question about Syringe [Re: Fahkface]
    #8092709 - 03/02/08 04:59 AM (16 years, 10 months ago)

I'm guessing providing you were clean in your procedures that some of them should turn out fine and any that don't just chock up to being new and don't even blame the syringes for being so small....it just makes them a pain in the ass ,it doesn't really make them unusable...Good luck:thumbup:

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InvisibleGretchenmeister
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Re: Question about Syringe [Re: Blutjager]
    #8092786 - 03/02/08 06:36 AM (16 years, 10 months ago)

Farm stores...like tractor supply...have all sized needles and syringes...generally near the horse feed area somewhere...I like a 60CC with a 18guage needle.


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Invisiblemyelination
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Re: Question about Syringe [Re: Gretchenmeister]
    #8092823 - 03/02/08 07:10 AM (16 years, 10 months ago)

if it was a spore syringe, the gauge of the needle was probably too small to pass many spores through. those insulin needles are usually really tiny.

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OfflineFahkface
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Re: Question about Syringe [Re: myelination]
    #8092869 - 03/02/08 07:52 AM (16 years, 10 months ago)

If he pushed in the whole syringe, it's likely, that enough spores got through the needle, so this shouldn't be a bigger problem.

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OfflineFexor
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Re: Question about Syringe [Re: Fahkface]
    #8095739 - 03/02/08 11:29 PM (16 years, 10 months ago)

Thanks all for your posts, I will keep you all up to date on how it goes! So far no signs of mycelium growth, it has only been a few days though,

I put 5 holes in each container so roughly 5 ml of spore solution in each, I know this would of increased my risk of contam but well we will see how it goes ^^

The liquid cultures though there is defiantly something growing in them, will get pictures soon.

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Offlinedestindiver1980
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Re: Question about Syringe [Re: Fexor]
    #8095769 - 03/02/08 11:37 PM (16 years, 10 months ago)

I am surprised your needles didn't get clogged. Those suckers get clogged if the littlest bit of crap gets in them. Hopefully it works out well for you. Oh, and didn't you know Tahoe is better than everyone. He's the best pro in the world, or so he thinks.


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:3some: Original poster of this is highc: 

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Offlinemozartvicious
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Re: Question about Syringe [Re: destindiver1980]
    #8095784 - 03/02/08 11:41 PM (16 years, 10 months ago)

I've never heard of a syringe with only 1cc
got a pic?


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Invisibletahoe
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Re: Question about Syringe [Re: Fexor]
    #8096676 - 03/03/08 08:49 AM (16 years, 10 months ago)

Quote:

Fexor said:
Thanks all for your posts, I will keep you all up to date on how it goes! So far no signs of mycelium growth, it has only been a few days though,

I put 5 holes in each container so roughly 5 ml of spore solution in each, I know this would of increased my risk of contam but well we will see how it goes ^^

The liquid cultures though there is defiantly something growing in them, will get pictures soon.



so you made a new spore syringe per hole??

Quote:

Fexor said:
And no I aint stupid



all evidence says otherwise


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread

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Invisiblemyelination
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Registered: 02/21/08
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Re: Question about Syringe [Re: mozartvicious]
    #8096693 - 03/03/08 08:58 AM (16 years, 10 months ago)

Quote:

mozartvicious said:
I've never heard of a syringe with only 1cc
got a pic?




here's a pic:


you can see that the needle is tiny! i can't imagine many spores sneaking through there.

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Invisibletahoe
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Re: Question about Syringe [Re: myelination]
    #8096799 - 03/03/08 09:47 AM (16 years, 10 months ago)

spore will fit just fine through there. I think they are 24-26 gauge needles.
They are basically idiot proof syringe for diabetics.
They are for muscle tissue injections and are short and skinny to prevent damage to nerves and to limit the soreness after injection.
They are almost too skinny for oil based injectable's

The needles are too short to for brf cakes that have verm layers. They would work great for tyvek lids.
They will not work for lc's


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread

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Invisiblemyelination
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Re: Question about Syringe [Re: tahoe]
    #8096855 - 03/03/08 10:08 AM (16 years, 10 months ago)

Quote:

tahoe said:
spore will fit just fine through there. I think they are 24-26 gauge needles.




a small point, but BD doesn't sell any insulin syringes with needles bigger than 28 gauge. some are as small as 31.

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Invisibletahoe
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Re: Question about Syringe [Re: myelination]
    #8096876 - 03/03/08 10:16 AM (16 years, 10 months ago)

shit, even thinner than I thought.
Spore would still fit through it but lc's no way.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread

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OfflineFexor
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Re: Question about Syringe [Re: tahoe]
    #8135335 - 03/12/08 01:47 AM (16 years, 10 months ago)

Well tahoe it seems you are incorrect so called Mr.know it all
All cakes have mycelium growing on them,

only one got contaminated, the Lcs have a heap of what I presume mycelium.. Although I am sketchy about using them, apparently it's hard to detect a contam with Lcs?.. I will use with one cake to begin with and see how it goes..

Thank you to the people that gave advice, and to people such as tahoe who can only criticize, and call people stupid when you do not even know me, you think your better then other people on here? yeah well your pathetic :yesnod::lol:

It seems people over exaggerate about the whole, sterile procedure?

Edited by Prisoner#1 (03/12/08 02:17 AM)

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Offlineshevanel
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Re: Question about Syringe [Re: Fexor]
    #8135394 - 03/12/08 02:14 AM (16 years, 10 months ago)

Who is tahoe?

I don't think the "whole sterile procedure" is over exagerated, but follwoing proper sterile technique helps save time I am sure. No need in being sloppy, waiting 2 weeks and end up back at square one from contams.

Afterall, you cannot really "see" contams so it's best to exert all available methods of keeping your shit clean so you have a better chance of not growing bullshit but rather growing what you'd had hoped to.

Good luck

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Offlineresptodd
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Re: Question about Syringe [Re: shevanel]
    #8135426 - 03/12/08 02:35 AM (16 years, 10 months ago)

No, no, no, don't mess with those syringes, they are worthless. And shevanel, you are exactly right clean/sterile procedure is a must, anyone saying otherwise is wrong, end of story.---You can get syringes at your local ag. store(tractor supply company, big acre), farmers stores,. All kinds and sizes of needles, syringes all kinds of crazy stuff, all packaged sterile too. The farmers use the stuff to vaccinate their livestock-It's the same shit Hospitals use, it's just packaged for veterinary use.


--------------------
Damn! I'm having fun! Just keep the GD monkeys away.

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