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InvisibleTheShroomJew23
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Registered: 11/29/07
Posts: 1,611
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Stronger Strains
    #8075050 - 02/26/08 10:23 PM (16 years, 24 days ago)

As more people dump their contamed casings and other stuff we get less cultures that have fought of infection and survived. Please, if you want stronger cultures let a contamed casing grow what ever tiny mushrooms it can. Culture them after that and they will be more resillient to contamination then before. I have a culture that has survived 10 contams and now it can fight off what ever I throw at it.


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Offlinexaxphaanes
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Re: Stronger Strains [Re: TheShroomJew23]
    #8075069 - 02/26/08 10:27 PM (16 years, 24 days ago)

Thats a lie and you know it cube mycelium will NOT fight off a contam.The contam either takes over .or the cube mycelium and the contam mycelium bond and grow together.Do Not follow this advice unless you want massive deposits of spores in your home which can ruin future grows.


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"Anything i say is fictional"
  what you should look for in manure

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InvisibleTheShroomJew23
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Re: Stronger Strains [Re: xaxphaanes]
    #8075078 - 02/26/08 10:29 PM (16 years, 24 days ago)

Thank you for saying that immediately, I put this out here as a way to show that things that sound logical are actually wrong.


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OfflineMYSTIQUE
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Re: Stronger Strains [Re: xaxphaanes]
    #8075082 - 02/26/08 10:29 PM (16 years, 24 days ago)

Ya I have never seen cube myc eat a contam. Post a video and prove us wrong time lapse thing then ill believe it.


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Dont know what the fuck I just said? READ THIS
http://www.shroomery.org/5122/The-Shroomery-Mushroom-Glossary
I ain't a hippy but I'm covered in dirt                         
Sippin lots of mushroom tea in a tye-dye shirt
Chasin' the Grateful Dead, no shoes on my feet
Beggin' in the parking lot for something to eat,

:onfire:DO NOT USE FIRE IN YOUR GLOVE BOX!!!!!!!:onfire:

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InvisibleRoYaL_fLuSh
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Registered: 01/04/08
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Loc: UK
Re: Stronger Strains [Re: TheShroomJew23]
    #8075086 - 02/26/08 10:30 PM (16 years, 24 days ago)

Quote:

TheShroomJew23 said:
As more people dump their contamed casings and other stuff we get less cultures that have fought of infection and survived. Please, if you want stronger cultures let a contamed casing grow what ever tiny mushrooms it can. Culture them after that and they will be more resillient to contamination then before. I have a culture that has survived 10 contams and now it can fight off what ever I throw at it.



it takes many a moon .. say 100s of thousands .. for substrains to evolve in that magnitude .. enjoy your luck while it lasts .. cheers

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Offlinexaxphaanes
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Re: Stronger Strains [Re: TheShroomJew23]
    #8075099 - 02/26/08 10:31 PM (16 years, 24 days ago)

Quote:

TheShroomJew23 said:
Thank you for saying that immediately, I put this out here as a way to show that things that sound logical are actually wrong.





You seriously are a jew!


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"Anything i say is fictional"
  what you should look for in manure

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InvisibleCrasher
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Re: Stronger Strains [Re: TheShroomJew23]
    #8075102 - 02/26/08 10:32 PM (16 years, 24 days ago)

Quote:

TheShroomJew23 said:
Thank you for saying that immediately, I put this out here as a way to show that things that sound logical are actually wrong.




:topicsucks:


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Give me silence, water, hope;
Give me struggle, iron, volcanoes...

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OfflineMYSTIQUE
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Re: Stronger Strains [Re: RoYaL_fLuSh]
    #8075123 - 02/26/08 10:36 PM (16 years, 24 days ago)

I have a culture that has survived 10 contams you say. is that g2g clone transfer or multi spore?

Either way you have messed up and been caught in a lie.

1. From spore a multi spore would not become stronger with each fruiting its a new start and would not make a super myc.

2. g2g 10 times with cloning would make the myc so old and weak that it would probably not even be able to colonize a jar let alone fight of a contam.

So stop spreading your lies.


--------------------
Dont know what the fuck I just said? READ THIS
http://www.shroomery.org/5122/The-Shroomery-Mushroom-Glossary
I ain't a hippy but I'm covered in dirt                         
Sippin lots of mushroom tea in a tye-dye shirt
Chasin' the Grateful Dead, no shoes on my feet
Beggin' in the parking lot for something to eat,

:onfire:DO NOT USE FIRE IN YOUR GLOVE BOX!!!!!!!:onfire:

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InvisibleTheShroomJew23
The Chemist
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Registered: 11/29/07
Posts: 1,611
Loc: Temple
Re: Stronger Strains [Re: MYSTIQUE]
    #8075140 - 02/26/08 10:40 PM (16 years, 24 days ago)

it was meant to be a lie, read the whole thread before you post.


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Offlinexaxphaanes
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Re: Stronger Strains [Re: TheShroomJew23]
    #8075145 - 02/26/08 10:41 PM (16 years, 24 days ago)

Dont make a post with lies? it is very confusing to newbies that are starting out.


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"Anything i say is fictional"
  what you should look for in manure

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InvisibleCrasher
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Re: Stronger Strains [Re: xaxphaanes]
    #8075149 - 02/26/08 10:42 PM (16 years, 24 days ago)

Quote:

xaxphaanes said:
Dont make a post with lies! it is very confusing to newbies that are starting out.




:zoolander: For Serious.


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Give me silence, water, hope;
Give me struggle, iron, volcanoes...

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OfflineMYSTIQUE
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Re: Stronger Strains [Re: TheShroomJew23]
    #8075156 - 02/26/08 10:44 PM (16 years, 24 days ago)

Quote:

TheShroomJew23 said:
it was meant to be a lie, read the whole thread before you post.




Your retarted. Think before you post a new thread.


--------------------
Dont know what the fuck I just said? READ THIS
http://www.shroomery.org/5122/The-Shroomery-Mushroom-Glossary
I ain't a hippy but I'm covered in dirt                         
Sippin lots of mushroom tea in a tye-dye shirt
Chasin' the Grateful Dead, no shoes on my feet
Beggin' in the parking lot for something to eat,

:onfire:DO NOT USE FIRE IN YOUR GLOVE BOX!!!!!!!:onfire:

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InvisibleRoYaL_fLuSh
Stranger
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Registered: 01/04/08
Posts: 499
Loc: UK
Re: Stronger Strains [Re: MYSTIQUE]
    #8075199 - 02/26/08 10:51 PM (16 years, 24 days ago)

:mrt: I PITY DA FOO DAT BELEVE DIS BULL SMACK !

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OfflineDr. Penguin
Organic Chemist
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Registered: 01/04/08
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Last seen: 12 years, 5 months
Re: Stronger Strains [Re: RoYaL_fLuSh]
    #8075360 - 02/26/08 11:32 PM (16 years, 24 days ago)

Ok, call me a complete noob but...

Cube myc fights off contams all the time in the wild. The species is no different correct? Shouldn't it be able, theoretically, to fight off an invader in laboratory conditions? What if I, through isolates, found a sub-strain that was strong enough to fight off a contamination. Yes it is unlikely, but it would be possible no?

And to the poster of this:
Quote:

the contam mycelium bond and grow together



If you can prove to me ANY contamination and P. Cubensis can bond together, I will publish the article.

People finish casings after contamination all the time. Maybe he found a particularly resilient strain.


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Offlinexaxphaanes
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Re: Stronger Strains [Re: Dr. Penguin]
    #8075442 - 02/26/08 11:57 PM (16 years, 24 days ago)

cubes dont fight off molds in nature they grow with it.


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"Anything i say is fictional"
  what you should look for in manure

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InvisibleSFsorrow
Is Born
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Registered: 11/11/07
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Re: Stronger Strains [Re: xaxphaanes]
    #8075462 - 02/27/08 12:01 AM (16 years, 24 days ago)

Mold in your 'laboratory' settings can NEVER be good. There is a reason why sterilization is stressed so hard on these forums, it really MUST be sterile!

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OfflineDr. Penguin
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Re: Stronger Strains [Re: Dr. Penguin]
    #8075531 - 02/27/08 12:20 AM (16 years, 24 days ago)

May I quote "Mr. Bloomfield's Orchard: The Mysterious World of Mushrooms, Molds, and Mycologists"

Page 142

Quote:

When different species and incompatible strains clash, the wood becomes stained with a pigment as the warring mycelia attack their opponents hyphe and produce thick melanin impregnated walls to resist each others poisons.



In fact if you place two species in a petri dish you can get them to fight. It is quite exciting...



I completely agree, mold is not good in a sterile environment, but it is not why we stress sterility. We are only trying to nurture ONE species in our cultures, even though they have identical ideal conditions as other, unwanted, fungi, thats why sterility is pressed. Casings are only pasteurized, not sterile correct?

I am not condoning the OP's information, I am only saying there is no need to completely throw his information out the window.


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InvisibleSFsorrow
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Re: Stronger Strains [Re: Dr. Penguin]
    #8075672 - 02/27/08 01:02 AM (16 years, 24 days ago)

I thought it was implicit (just by the name of this forum) what the desired outcome of our collective experiences are. :wink:

Isolating desired traits in our beloved fungus is without question a noble undertaking but the means the OP originally posted is just absurd and SHOULD be thrown out in just about every fruiting condition.

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OfflineFahkface
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Re: Stronger Strains [Re: SFsorrow]
    #8076006 - 02/27/08 04:18 AM (16 years, 24 days ago)

If you have a contam either in the wild or in laboratory environments, the cube mycelium doesn't actually "fight" the contams, but kind of tolerates it or prevents it from further growing by increasing it's own efforts, to get as many nutrients as possible.
It won't overtake the moldy parts and it won't fight it off in a way of reusing the mold infested parts of the substrate. At least I've never seen it.

Let's say you have a fully colonized casing that has already fruited and you detect mold stains, this would be a sign for the mycelium not be the strongest. So letting this grow would probably result -beside the mold infection of your apartments- in the opposite of what you suggested.
The mycelium won't recover and it won't develop stronger genes.
As Royal already said, this would take maybe thousands of grows.
Therefore clones and isolates are used...

Of the billions of mushroom spores, that fly around in nature, only VERY few are able to actually germinate and successfully develop mycelium and mushrooms.
Beside strong genes they have to be VERY "lucky" to "find" the right circumstances that support their growth.
If spores with strong genes land on a fresh pile of cow dung, which is probably completely infested with bacteria etc. the mushrooms just has to be faster than the mold spores and develop strong mycelium that's more resistant to molds and bacteria.
If mold gets a grip on this pile, it's unlikely for the mushroom to develop as much myc and fruits as if it would do, when there was no mold, for it has to concentrate on developing more mycelium and to "conquer" as much substrate as possible.
From what I've seen so far, mold grows when the mycelium isn't actually fully developed OR it has already produced fruits and is exhausted, so it has not enough energy to protect itself from an contam attack, anymore.
In the end -for me- it has always ended in a "victory" of the mold. No recovery and no long life...

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Offlineskattman1982
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Re: Stronger Strains [Re: TheShroomJew23]
    #8076194 - 02/27/08 07:39 AM (16 years, 24 days ago)

Quote:

TheShroomJew23 said:
Thank you for saying that immediately, I put this out here as a way to show that things that sound logical are actually wrong.




double talk is it? why post this as a topic then say you were only kidding? does not make sense.


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They lie about marijuana. Tell you pot-smoking makes you unmotivated. Lie! When you're high, you can do everything you normally do, just as well. You just realize that it's not worth the fucking effort. There is a difference.-Bill Hicks-

by the way, everything i write is for entertainment purposes and is purely fictional. do not construe these written words with the author having any knowledge of actual persons, places, events, or actions.

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