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OfflineManianFHS
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Must add water to jars... impertinent
    #8070393 - 02/25/08 09:57 PM (15 years, 11 months ago)

Ok so I dehumidified my jars, specifically because I DID NOT take RR's advise and get my jars out of the PC that I was using as an incubator. Now I have these sterile jars with no water in them....

So, I am going to pull sterile water from a bottle of distilled water with a syringe, by pushing the needle in through the side of the bottle. I am going to place cyran wrap over the jars in a sterile environment, and inject 4.5CC of water into each jar, then shake vigorously...

Anyone have another suggestion?


--------------------
notapillow said: "you are going about this endeavor all wrong. clear your mind of useless fear and concern. buy the ticket, take the ride, and all that.... "

ChrisWho said: "It's all about the journey, not the destination."


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OfflineBlargIAmDead
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Re: Must add water to jars... impertinent [Re: ManianFH]
    #8070409 - 02/25/08 10:01 PM (15 years, 11 months ago)

Make new jars. Not worth monkeyin' with water content. Make new jars w/ the correct water content. Don't incubate. Repeat until expectations are met.


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OfflineManianFHS
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Re: Must add water to jars... impertinent [Re: BlargIAmDead]
    #8070427 - 02/25/08 10:06 PM (15 years, 11 months ago)

well not making new jars, due to time and privacy constraints, this is either happening or not. So ill either have shrooms or really colorful photos to show in the contamination forum.


--------------------
notapillow said: "you are going about this endeavor all wrong. clear your mind of useless fear and concern. buy the ticket, take the ride, and all that.... "

ChrisWho said: "It's all about the journey, not the destination."


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Invisibleim_on_a_boat
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Re: Must add water to jars... impertinent [Re: ManianFH]
    #8070449 - 02/25/08 10:09 PM (15 years, 11 months ago)

post some pics so we can see how dry they are man..

are they grain?

otherwise i wouldn't suggest shaking them..

how dry are they really? i've heard the drier they are, the faster they colonize (to a point, that is)


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OfflineManianFHS
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Re: Must add water to jars... impertinent [Re: im_on_a_boat]
    #8070519 - 02/25/08 10:25 PM (15 years, 11 months ago)

grain/hpoo mix.

dry enough that when I turn one upside down, all the contents simply fall... no sticking at all...
i will post pics but right now I got a cat on me and thus am forced to surf the internet


--------------------
notapillow said: "you are going about this endeavor all wrong. clear your mind of useless fear and concern. buy the ticket, take the ride, and all that.... "

ChrisWho said: "It's all about the journey, not the destination."


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Invisibleim_on_a_boat
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Re: Must add water to jars... impertinent [Re: ManianFH]
    #8070528 - 02/25/08 10:27 PM (15 years, 11 months ago)

that's a good thing i would think.. if they stick together that means you either didn't rinse them off enough or they are too wet..

why did you put hpoo in the jars, why not just wait and then spawn?

i thought you're supposed to pasteurize it anyways, is pc'ing it even pasteurizing it?

i dont know much about hpoo..


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OfflineManianFHS
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Re: Must add water to jars... impertinent [Re: im_on_a_boat]
    #8070617 - 02/25/08 10:45 PM (15 years, 11 months ago)

I pasteurized the hpoo, then pcd it (probably pc wouldve sufficed, I wanted the extra moisture from pasteurization..). I used the mix because I wont be spawning to substrate, rather just dunking the cakes out of the jars and inducing pin growth. Was hoping to write up a nice tek on it, but if I cant get it right, its not going to happen ;p.

Heres some pics. I have 4 jars.. Im thinking of adding water to two of them, and leaving the other ones alone. even if one jar colonized properly id be happy.
heres the pics i promised, taken on a 2mpx cameraphone:






--------------------
notapillow said: "you are going about this endeavor all wrong. clear your mind of useless fear and concern. buy the ticket, take the ride, and all that.... "

ChrisWho said: "It's all about the journey, not the destination."


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Invisibleim_on_a_boat
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Re: Must add water to jars... impertinent [Re: ManianFH]
    #8070644 - 02/25/08 10:50 PM (15 years, 11 months ago)

doesn't look bad at all to me.. i cant really tell how much moisture there is, though..

if you had a colonized jar already and could do g2g, i would suggest that.. that would prob colonize that shit awesomely..

and why would you want to make a cake out of it?  there's a reason that you do casings with bird seed instead of cakes :laugh:

i would prob just knock it up instead of fckin with the water content.. but, then again, i dont know how dry those bitches are either...

good luck whatever you do.


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Invisibledumbfounded1600
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Re: Must add water to jars... impertinent [Re: im_on_a_boat]
    #8070713 - 02/25/08 11:03 PM (15 years, 11 months ago)

heh..good luck


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OfflineManianFHS
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Re: Must add water to jars... impertinent [Re: im_on_a_boat]
    #8070751 - 02/25/08 11:10 PM (15 years, 11 months ago)

Thanks for the input. I have read that mushrooms grow with more potency from horsepoo than from WBS alone. Since I cannot spawn this to substrate (well just leave it at that) I wanted to give my mushrooms the best chance at having some potency straight off the cakes.

Anyways... its been about 7 days since I inoculated. Not even a bit of growth, and this is with 3 days of initial incubation.

Either im going to open one of the jars (ruining it) to check the water content (because it is hard to tell how dry these things are, even by teh naked eye), or simply add water by syringe to (2) of them.


--------------------
notapillow said: "you are going about this endeavor all wrong. clear your mind of useless fear and concern. buy the ticket, take the ride, and all that.... "

ChrisWho said: "It's all about the journey, not the destination."


Extras: Filter Print Post Top
Invisibledumbfounded1600
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Registered: 07/29/07
Posts: 2,624
Re: Must add water to jars... impertinent [Re: ManianFH]
    #8070830 - 02/25/08 11:31 PM (15 years, 11 months ago)

Potency is genetic. Get used to it. Especially with multispore inoculation. It'll vary from flush to flush. BRF has more nutrition than any other common bulk substrate.

If you want consistent potency do isolates on agar.


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OfflineEngineer
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Registered: 02/25/08
Posts: 56
Last seen: 15 years, 10 months
Re: Must add water to jars... impertinent [Re: dumbfounded1600]
    #8070887 - 02/25/08 11:48 PM (15 years, 10 months ago)

Yes, don't worry about potency (genetic). The millet grains look plump (IMHO), which is a good sign. If I were to add water, I would PC the syringe, wrapped in Al foil, along with a jar of water with a suitable port to allow aspiration of the water via the syringe. Then, using whatever aseptic techniques you like, fill syringe and squirt into jars. I'd wait for the mycelium to stop growing for awhile though first...if it keeps growing let it!


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