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Offlinenibelle
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Question for RR - spores on agar
    #8068423 - 02/25/08 12:32 PM (16 years, 26 days ago)

Hi RR.

I got your video and I have watched all of it. I have been reading around here for many weeks now while I was waiintg for my prints.

I have agar supplies and a shoebox. I was just wondering, since I can't find on the videos a place where you actually use spores to inoculate agar directly, what was the method you are use? I also have an inoculation loop.


Thanks! comments from others are welcome!

Edited by nibelle (02/25/08 12:50 PM)

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OfflineMYSTIQUE
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Re: Question for RR - spores on agar [Re: nibelle]
    #8068530 - 02/25/08 01:10 PM (16 years, 26 days ago)

Flame your loop and touch it to your spore print.

Move it back and forth a little if you need to but just touching it should give you tons.

In a glove box open the lid to the dish just enough to get the loop in without touching the lid.

Do a zig-zag pattern back and forth lightly on the agar to get a nice thin smear at the end and easier to separate.

You can also touch it to the middle of the agar and let it grow from there.

Then section till you run out of fridge space and don't forget to label and double label everything color code and water proof marker just in case cuz nothing will suck more then mixing it all up.


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Offlinenibelle
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Re: Question for RR - spores on agar [Re: MYSTIQUE]
    #8068597 - 02/25/08 01:32 PM (16 years, 26 days ago)

Ur an angel baby :smile: kiss xxx

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OfflineRogerRabbitM
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Re: Question for RR - spores on agar [Re: nibelle]
    #8068803 - 02/25/08 02:48 PM (16 years, 26 days ago)

There is a demonstration of inoculating agar with spores in the agar/petri dish section. Use a flame sterilized inoculating loop, as said above.
RR


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