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OfflineSamurai_Jim
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Registered: 01/12/08
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One good jar of grain. What is best to do with it?
    #8067834 - 02/25/08 08:02 AM (15 years, 11 months ago)

I have a good jar of rye grain of myc. It is now fully colonized. I don't have a flow hood built yet, but I do have a still air/glove box. What would be best to do with the jar?

Should I do a G2G transfer on a few jars of grain, case it in a small monotub, refrigerate it for use later? Suggestions please.


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OfflineCrazyEarl
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Re: One good jar of grain. What is best to do with it? [Re: Samurai_Jim]
    #8067843 - 02/25/08 08:09 AM (15 years, 11 months ago)

i would spawn to some sub and take clones from clusters of fruits. then grow each clone out to see which one fruits the best.


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Invisiblear393
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Re: One good jar of grain. What is best to do with it? [Re: Samurai_Jim]
    #8067845 - 02/25/08 08:09 AM (15 years, 11 months ago)

G2G.

Definately G2G if you want to do a bigger monotub later.


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OfflineTerillius
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Re: One good jar of grain. What is best to do with it? [Re: Samurai_Jim]
    #8067909 - 02/25/08 08:49 AM (15 years, 11 months ago)

Your idea would be the best use. G2G and a small casing. You could fruit it right out of the jar if you are lazy. Not a bad way to do it and it looks pretty cool when all the fruits are growing up out the top.


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OfflineSamurai_Jim
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Re: One good jar of grain. What is best to do with it? [Re: Terillius]
    #8067963 - 02/25/08 09:23 AM (15 years, 11 months ago)

Can one successfully do a G2G in a glovebox/still air box?


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Offlinebwoy
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Re: One good jar of grain. What is best to do with it? [Re: Samurai_Jim]
    #8068089 - 02/25/08 10:10 AM (15 years, 11 months ago)

Spawn to a bag of hpoo, quick colonization, fruit in RR perlite chamber, no fuss, no effort, little risk of contams. 300-500g yield.


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Invisibleblood4blood
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Re: One good jar of grain. What is best to do with it? [Re: bwoy]
    #8068114 - 02/25/08 10:21 AM (15 years, 11 months ago)

http://www.shroomery.org/forums/showflat.php/Cat/0/Number/8064016/an/0/page/2

i did these in a glove box. turned one jar into 5. only got pictures of three thuogh. g2g would be my only suggestion with that 1 jar.


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Offlinexhooliganx
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Re: One good jar of grain. What is best to do with it? [Re: blood4blood]
    #8068183 - 02/25/08 10:52 AM (15 years, 11 months ago)

i've done all of my g2g work and agar work in a glove box. you should be able to make 10 new jars with that one.


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OfflineNeobean
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Re: One good jar of grain. What is best to do with it? [Re: blood4blood]
    #8068187 - 02/25/08 10:55 AM (15 years, 11 months ago)

Quote:

blood4blood said:
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/8064016/an/0/page/2

i did these in a glove box. turned one jar into 5. only got pictures of three thuogh. g2g would be my only suggestion with that 1 jar.




I agree with this guy. 1 to 5...great ratio. Also, wat you can do is put some of it (few grains) on agar, to isolate the most ryzo strain.


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OfflineSamurai_Jim
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Re: One good jar of grain. What is best to do with it? [Re: Neobean]
    #8074338 - 02/26/08 08:03 PM (15 years, 10 months ago)

Well, I did this fellas:


Took the 1 jar into 2 quarts, 2 pints and 1 agar. I still have a bit left in the bottom of the jar, but I'll just case it and have some fun. The two pints are going to be for Liquid transfer, sterilized water style and the two jars will be for more G2G on a bit larger scale.


BTW, this strand is creeper or texas, I forgot which one.


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Invisiblepsiloglow
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Re: One good jar of grain. What is best to do with it? [Re: Samurai_Jim]
    #8074698 - 02/26/08 09:18 PM (15 years, 10 months ago)

Transfer a chunk to a spawn bag of rye grain, a few times over. Create 10 jars from one at least. its possible. No flowhood needed. Just a clean room.


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Offlinevinylcassett
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Re: One good jar of grain. What is best to do with it? [Re: psiloglow]
    #8074775 - 02/26/08 09:30 PM (15 years, 10 months ago)

does everyone use a glove box? I just do it in my bathroom after disinfecting. So far i've had zero contams.


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Invisiblepsiloglow
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Re: One good jar of grain. What is best to do with it? [Re: vinylcassett]
    #8074817 - 02/26/08 09:38 PM (15 years, 10 months ago)

exactly vinyl....a little bleach spray mist and perhaps a hape filter for an hour, and you got a huge glove box...


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InvisibleGretchenmeister
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Re: One good jar of grain. What is best to do with it? [Re: Samurai_Jim]
    #8075121 - 02/26/08 10:36 PM (15 years, 10 months ago)

oh i would make some lc...shoot clean water into the colonized jar with clean syringe, shake around a bit, suck back out...and add to a LC mixture and make shittons of noc up material for pennies! I like to use barley malt syrup and fructrose LC...there are some good recipes out there. Make more than one LC...in case you lose a couple!


Edited by Gretchenmeister (02/26/08 10:38 PM)


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