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juggaloskater
Stranger

Registered: 02/24/08
Posts: 62
Last seen: 12 years, 4 months
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So far unsuccessful first try... advice please
#8064765 - 02/24/08 02:33 PM (15 years, 11 months ago) |
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Hello everybody, this is my first post on here and my first time trying to grow fungus. So here's the deal, I ordered 2 amazonian syringes from sporestore.com (from which i have now read bad reviews). After waiting a long time.... i finally received the spores and I went to work. I couldn't find BRF so I just grounded up some in my coffee grinder, which left some big chunks that still looked like pieces of rice. I pre-sterilized the verm and rice flour by sticking them in the oven for about 20 mins at 350. During the mixing, I made the mistake of throwing everything together at the same time so there were some clumps of rice. I put about 2:1 verm to rice, and a bit more water than rice and I also mixed in a bit of honey with the hot water. BTW, the verm is fine type. Anyways, I continued to make the jars with the layer of dried verm on top, then boiled for about an hour and 15 then and let it cool overnight (about 7-8 hours) and again I made a mistake by inoculating while the jars were still warm to the touch. About a week later, I saw no signs of growth (neither bacteria nor mycelium) so I proceeded to use the other syringe and inoculate the jars again (I assumed the heat had killed the spores). It has been about 10 days since then and I still see no signs of growth. I went ahead and ordered another syringe from spores101.com just in case. So here's the big question... when the other syringe gets here, if there is still no sign of growth what should I do? Should i try to inoculate the same jars since there is no contams? or should I throw those out and go ahead and re-do the whole thing? Thanks in advance for the help.
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TobesCanaian



Registered: 12/05/07
Posts: 114
Loc:
Last seen: 3 years, 8 months
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Re: So far unsuccessful first try... advice please [Re: juggaloskater]
#8064805 - 02/24/08 02:47 PM (15 years, 11 months ago) |
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I would read the Pf tek or check out the Pf tek video on you tube. No where have i ever read to put honey into a jar to me thats asking for contams. Steam sterilizing is better then oven sterlizing, Jars can take up to 2 weeks to show anything so maybe you should just let them go and wait to see whats going on. Never reuse a jar if it doesnt work. If it didnt work the first time then it wont work the next time. I would suggest starting over and read more and not just go with what you think there is actual measurements that can be used for newbies
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juggaloskater
Stranger

Registered: 02/24/08
Posts: 62
Last seen: 12 years, 4 months
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Re: So far unsuccessful first try... advice please [Re: TobesCanaian]
#8064836 - 02/24/08 02:56 PM (15 years, 11 months ago) |
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well... the honey thing I got from the Shroom Wizard's site, and the 2:1 ratio I got from a video called "Let's Grow Mushrooms!" and the extra bit of water I added was because the The Magic Mushrooms Growing Guide calls for extra water if you grind up your own flour. Also, I did the steam sterilizing after the oven sterilization.
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TobesCanaian



Registered: 12/05/07
Posts: 114
Loc:
Last seen: 3 years, 8 months
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Re: So far unsuccessful first try... advice please [Re: juggaloskater]
#8064842 - 02/24/08 02:59 PM (15 years, 11 months ago) |
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Then give them time see what happens i got my first set of syringes from that place as well and i had only good results I still have Wbs colonzied with it from G2G transfers. Patience is the key to growing. how much of each syringe are you putting in?
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juggaloskater
Stranger

Registered: 02/24/08
Posts: 62
Last seen: 12 years, 4 months
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Re: So far unsuccessful first try... advice please [Re: TobesCanaian]
#8064845 - 02/24/08 03:01 PM (15 years, 11 months ago) |
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I put about 1 cc per jar... a bit less since I had 11 jars
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TobesCanaian



Registered: 12/05/07
Posts: 114
Loc:
Last seen: 3 years, 8 months
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Re: So far unsuccessful first try... advice please [Re: juggaloskater]
#8064856 - 02/24/08 03:04 PM (15 years, 11 months ago) |
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I would just say leave em alone and see where it goes, I am not expert on the pf jars i prefer Wbs personally but i know you should wait no matter what substrate your knocking up
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Frost000
Badfish

Registered: 01/03/08
Posts: 236
Last seen: 10 years, 11 months
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Re: So far unsuccessful first try... advice please [Re: TobesCanaian]
#8064882 - 02/24/08 03:16 PM (15 years, 11 months ago) |
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Just because you don't see contamination doesn't mean it's not there. Also, since you've never grown before, STICK TO THE TEK. Don't use honey. Start over(10 days isn't really long enough to rule it out as a bust though). RogerRabbits video and posts will be your best friend while learning to cultivate.
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thatamatt
reaching for the secret


Registered: 09/16/07
Posts: 168
Loc: Pangaea
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Re: So far unsuccessful first try... advice please [Re: Frost000]
#8065013 - 02/24/08 03:57 PM (15 years, 11 months ago) |
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Quote:
Frost000 said: Just because you don't see contamination doesn't mean it's not there. Also, since you've never grown before, STICK TO THE TEK. Don't use honey. Start over(10 days isn't really long enough to rule it out as a bust though). RogerRabbits video and posts will be your best friend while learning to cultivate.
Precisely, I did not see any mycelium until 15 days, on my first grow.
-------------------- i cry for the moon
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juggaloskater
Stranger

Registered: 02/24/08
Posts: 62
Last seen: 12 years, 4 months
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Re: So far unsuccessful first try... advice please [Re: Frost000]
#8065014 - 02/24/08 03:57 PM (15 years, 11 months ago) |
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Thanks for the replies. I guess I'll give it until the new syringe arrives, and if there is still no sign of life I will dump out what I have and make new substrate with no honey.
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