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OfflineDerk
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Registered: 01/10/08
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Starting my 1st grow try tomorrow..few ?s
    #8064762 - 02/24/08 02:33 PM (15 years, 11 months ago)

Should I do the verm/brown rice method or grain method? I have supplies for both but I don't know which one if more n00b proof. I'm going out tomorrow to get all the supplies. I have a few questions though.

Can I find the brown rice flour at HEB or Krogers?

How do you put filters on the jars? Do you just put them over the top and hold it down with a rubberband or is the filter suppose to go under the jar lid? I'm guessing using coffee filters works for this.

If using WBS, it says to leave room in the jar to shake. Well then how are you suppose to inject the grain if the needle won't be able to reach inside the grain since you have to leave extra space to shake?

I'm thinking I'm going to do WBS because I already have a PC.


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OfflineMrbob
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Registered: 01/17/08
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Re: Starting my 1st grow try tomorrow..few ?s [Re: Derk]
    #8064808 - 02/24/08 02:48 PM (15 years, 11 months ago)

im a noob myself and ur suppose to shake after injection on the wbs.

Good tek to follow is Doc's


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I have no post because i use the search button, duh!


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OfflineTobesCanaian
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Re: Starting my 1st grow try tomorrow..few ?s [Re: Mrbob]
    #8064830 - 02/24/08 02:53 PM (15 years, 11 months ago)

I personally only use Wbs i failed with the Pf jars do to temp fluc, I personally use coffee filters as well for my filter i put it between the lid and the cap of course with the hole in the lid, when i do my inoculation i just quickly open the jar up and give it a shot of syringe and the close it up, i have a sterile enviroment and never get any contams as long as your quick and are clean. You need to leave extra space so you can shake it when its partially colonizes for faster colonzation of the rest. I would myself if its your first time using Wbs do a couple of jars that you shake after innoc and a couple of jars you just let sit and determine which one works better for you.


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OfflineFrost000
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Registered: 01/03/08
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Re: Starting my 1st grow try tomorrow..few ?s [Re: TobesCanaian]
    #8064871 - 02/24/08 03:11 PM (15 years, 11 months ago)

PF tek is less prone to contamination once put into fruiting conditions.

As for the filter lids, use tyvek. You can get it at te post office or lowes(tyvek coveralls). I don't believe coffee filters would work too well. Also, don't put it under the lid, as it will be more prone to getting wet when you shake(although grains should not appear wet), thereforer causing contamination.

When using grain, there should be 3 times you should shake your jars. Once when you inoculate, once at 20-30% colonized and once when you case.

Also, make sure you drill some holes in the lids to provide adequete gas exchange. You can inoculate through one of the holes and cover with micropore tape. Best of luck to you.


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OfflineJadis
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Re: Starting my 1st grow try tomorrow..few ?s [Re: Frost000]
    #8064888 - 02/24/08 03:20 PM (15 years, 11 months ago)

from experience in the lab, 2 layers of coffee filters can be used to maintain sterility from airborne contams and still provide fresh air.

i havent tried this with PF jars, but that's how we grow aerobic bacteria in the lab sometimes to keep other contaminants out

edit: but since tyvek is so cheap you should prob just use that and not risk it. i suppose sterility is less of an issue when working with bacteria broths that prob eliminate foreign organisms quickly..


--------------------
Do you realize that everyone you know someday will die?
And instead of saying all of your goodbyes,
Let them know you realize that life goes fast.
It's hard to make the good things last.
You realize the sun don't go down;
It's just an illusion caused by the world spinnin' round.


Edited by Jadis (02/24/08 03:23 PM)


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OfflineDerk
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Re: Starting my 1st grow try tomorrow..few ?s [Re: Jadis]
    #8065008 - 02/24/08 03:56 PM (15 years, 11 months ago)

So in what section would this tyvek be in?

Also, when inoculating, can I just dump my spores onto the top of the grain and then shake it up to mix it all over the place? My needle isn't that long.


Edited by Derk (02/24/08 04:03 PM)


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Offlinefish42
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Re: Starting my 1st grow try tomorrow..few ?s [Re: Derk]
    #8065062 - 02/24/08 04:11 PM (15 years, 11 months ago)

go to the post office, it's free there


--------------------
Do you dare to feel responsible for every dollar you lay down
are you going to make the rich man richer
or are you going to stand your ground
You say you want a revolution
a communal evolution
to be a part of the solution
maybe I'll be seeing you around.


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OfflineFrost000
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Registered: 01/03/08
Posts: 236
Last seen: 10 years, 11 months
Re: Starting my 1st grow try tomorrow..few ?s [Re: fish42]
    #8065089 - 02/24/08 04:21 PM (15 years, 11 months ago)

Yes, inject the spores. Then shake the jar slightly to distribute them throughout the grain. The tyvek coveralls would be in the paint section.


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OfflineHeadFood
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Registered: 12/30/07
Posts: 390
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Re: Starting my 1st grow try tomorrow..few ?s [Re: Derk]
    #8065335 - 02/24/08 05:32 PM (15 years, 11 months ago)

Quote:

Derk said:
My needle isn't that long.




:rofl2:


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Phi Alpha


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Offlineismokeweed
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Registered: 11/19/05
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Re: Starting my 1st grow try tomorrow..few ?s [Re: HeadFood]
    #8065633 - 02/24/08 06:38 PM (15 years, 11 months ago)

hahaha headfood. you perv. (if I'm reading right. If not, apparently im the perv) hah. either way I'm still gettin a kick out of it.


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