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SuchSmartMonkeys
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Registered: 10/26/05
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Last seen: 2 years, 10 months
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risk of contam switching lid on incubating trays?
#8061242 - 02/23/08 04:26 PM (15 years, 11 months ago) |
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trays filled up on sunday, and rye spawn added and mixed with coir/coffee/gypsum bulk sub (the bulk sub was pasteurized). I duct taped the outsides of the trays, except for the bottom, which i have been lifting and looking at to see how colonization is coming along. I just placed the lids that came with the trays on them, and i've realized that this might not give them enough chance to breathe. The tops sit on top, seems like some AE should be possible, but how much of a risk of contam would it be for me to take the lids off and replace with aluminum foil with holes and microporous tape over them? looking at the bottoms, they look about 50% colonized... would i be better off just waiting to see what happens, or trying to switch to better covers?
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SuchSmartMonkeys
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Re: risk of contam switching lid on incubating trays? [Re: SuchSmartMonkeys]
#8062479 - 02/23/08 10:03 PM (15 years, 11 months ago) |
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sorry, i don't like bumping.... but anyone got advice on this? it seems like they've been slowing down a little bit, i don't want them to stall due to too much co2... i'm so fuckin close, i can taste it, i don't want to ruin it now...
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FooMan




Registered: 02/02/05
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Re: risk of contam switching lid on incubating trays? [Re: SuchSmartMonkeys]
#8062494 - 02/23/08 10:09 PM (15 years, 11 months ago) |
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You don't want any fresh air exchanges at this point. You simply want some of the CO2 buildup to be able to escape during this phase. Unless the lid you're using is air tight, it should be providing enough of this gas exchange for the mycelium to finish colonizing. When you are ready to fruit, the sudden drop in CO2, along with light, lower temps, etc will help trigger pinning.
Edited to add: If they're stalling you may want to just lift the lid slightly on one side so that it's still resting on the bin, just not tightly.
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Edited by FooMan (02/23/08 10:11 PM)
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SuchSmartMonkeys
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Re: risk of contam switching lid on incubating trays? [Re: FooMan]
#8062505 - 02/23/08 10:14 PM (15 years, 11 months ago) |
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thanks a lot, i just needed a response like that. i know they can tolerate a lot of co2 during colonization, but i wasn't certain how much... i've had jars stall before, and the bulk sub is much bigger, but the lids are on fairly loosely. should be getting enough. i'll wait a few more days still before i open it to see how much longer it needs to go until casing...
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RogerRabbit
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Re: risk of contam switching lid on incubating trays? [Re: SuchSmartMonkeys]
#8064134 - 02/24/08 11:38 AM (15 years, 11 months ago) |
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Nothing in mycology should be 'incubated'. I can't think of another easily avoidable point of failure than using silly ass incubators. Use room temp.
Don't change lids. It's best to avoid fixing what isn't broken. As foo said, air exchange is a pinning trigger. As long as the excess gasses can find a way to seep out, you're fine. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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SuchSmartMonkeys
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Registered: 10/26/05
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Re: risk of contam switching lid on incubating trays? [Re: RogerRabbit]
#8064392 - 02/24/08 12:42 PM (15 years, 11 months ago) |
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ah, harsh.... i'm not really using an incubator per se, just for lack of better words... Room temps fluctuate all together way too much in the house i'm at (roomies turn off heat at night, it gets real cold, then gets pretty warm during the day time...) i've just got them sitting in my FC, my FC happens to have a heat mat in there that is keeping them right at about 80 degrees while it spawn runs... when it comes time to fruit, i will turn it down/off
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RogerRabbit
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Re: risk of contam switching lid on incubating trays? [Re: SuchSmartMonkeys]
#8064542 - 02/24/08 01:24 PM (15 years, 11 months ago) |
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80F is too hot for colonization of bulk substrates. The interior of your substrate is well over 90F when ambient is 80F. In addition, never heat fruiting chambers. It causes too much condensation. A difference in day/night temps doesn't harm anything. It's natural. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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tahoe
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Re: risk of contam switching lid on incubating trays? [Re: RogerRabbit]
#8064702 - 02/24/08 02:11 PM (15 years, 11 months ago) |
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Quote:
RogerRabbit said: 80F is too hot for colonization of bulk substrates. The interior of your substrate is well over 90F when ambient is 80F. In addition, never heat fruiting chambers. It causes too much condensation. A difference in day/night temps doesn't harm anything. It's natural. RR
Check these out, these are oysters on wood chips so they are producing some eat trying to break the wood down

room temp

sub temp
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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SuchSmartMonkeys
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Registered: 10/26/05
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Re: risk of contam switching lid on incubating trays? [Re: tahoe]
#8065522 - 02/24/08 06:15 PM (15 years, 11 months ago) |
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alright, alright.....but your room temp is around 70, mine is around 55-60.... i'll go turn it way down for colonization.
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