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Offlinetagskill
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Registered: 02/18/08
Posts: 4
Last seen: 9 years, 3 months
Mycelium cloning
    #8055827 - 02/22/08 10:26 AM (15 years, 11 months ago)

Im abit new to this and i was wondering if it was possible to go without a syringe or a spore print for some months.
One method i wanted to see if it would work is taking mycelium from fully colonized jars and putting them in uncolonized jars to create a neverending cycle


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InvisibleTomandjerry58
Stranger
Registered: 01/27/03
Posts: 5,212
Re: Mycelium cloning [Re: tagskill]
    #8055842 - 02/22/08 10:30 AM (15 years, 11 months ago)

its called a grain 2 grain transfer ... but if you wanted TO do it right you need to start agar cultures.... and by the way WELCOME!!!!!


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Offlinetagskill
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Registered: 02/18/08
Posts: 4
Last seen: 9 years, 3 months
Re: Mycelium cloning [Re: Tomandjerry58]
    #8055870 - 02/22/08 10:37 AM (15 years, 11 months ago)

Thanks ^_^. so what you're saying is that its not as easy as just distributing the cake pieces to other substrates? like that WILL not work?


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InvisibleTomandjerry58
Stranger
Registered: 01/27/03
Posts: 5,212
Re: Mycelium cloning [Re: tagskill]
    #8056034 - 02/22/08 11:21 AM (15 years, 11 months ago)

i thought you were taking about making jars... when people say substrate they mean like poo or vermiculite whatever you are spawning too.. check out the search function .... and also check out the mushroom cultivation archive... some really neat stuff in there.... good luck


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InvisibleSmushroom
Avid Learner
Registered: 02/02/05
Posts: 2,806
Re: Mycelium cloning [Re: Tomandjerry58]
    #8056564 - 02/22/08 01:42 PM (15 years, 11 months ago)

Nothing is "impossible" in this hobby. However using cakes and trying to transfer from one to the other is pretty much as close as you can get.

When you use a sterile substrate it is free of any helpful bacteria to fight off contams so if even the smallest amount of mold spores got in when you were adding the previously colonized cake then it would take hold and ruin the jar.

If you were using whole grains like wbs (wild bird seed) or rye you can do a g2g (grain to grain) transfer and keep it going pretty long, but you have to be super sterile about it using atleast a glovebox or still air box and preferably a flow hood.

Now you could use your cakes to spawn to other substrates but they would almost definitely need to be pasteurized, not sterilized. Pasteurization will kill off the existing contams but leave the beneficial bacteria to allow the myc time to colonize it. Grains can't be pasteurized so you would need to use a bulk substrate like straw, poo, coir/coffee, or compost. Going bulk has some problems and may be easy to mess up if you have only been using cakes for a short time and never really did grain. Grain is a much better spawn for bulk substrates than cakes are.

If you just want to keep using cakes or maybe start to experiment with grains it would be best to create a LC (liquid culture). You can either do it from a print/syringe or for the best results you could grow on agar and transfer that to the LC. Agar work can be tedious though, especially if you just want to do this as a low scale hobby. Something you can try which may or may not work is to try and clone a fresh mushroom directly into your LC. I am just trying it myself and can't confirm how easily it can be done. If you are interested read this post http://www.shroomery.org/forums/showflat.php/Number/8028506#Post8028506

Once you create a LC you can keep it around for quite a while. A pint of LC can be used to create 20-30 syringe fulls. It can also be covered with foil and kept in the refrigerator for a few months pretty safely.


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