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Offlineshevanel
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grain to grain, liquid culture, agar, discuss. *DELETED*
    #8053982 - 02/21/08 09:14 PM (15 years, 11 months ago)

Post deleted by shevanel

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Edited by shevanel (02/21/08 09:16 PM)


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Invisibleim_on_a_boat
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Re: grain to grain, liquid culture, agar, discuss. [Re: shevanel]
    #8054010 - 02/21/08 09:18 PM (15 years, 11 months ago)

i'd put it in advanced..

i'd just keep prints for long term and use g2g for short term.. just keep it running.. g2g is awesome once you get the hang of it..

dont have any experience with agar or anything because i've never had to..

that's more if you want to isolate strains and all that good stuff i would think.. i just stick to multispore.

if you wanted to do that shit, agar is definitely the way to go..

and i dont use a box/hood/nothing.. just do straight air g2g.. with a spoon even.. haven't had any grain contams yet..


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InvisibleThis guy
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Re: grain to grain, liquid culture, agar, discuss. [Re: shevanel]
    #8054029 - 02/21/08 09:22 PM (15 years, 11 months ago)

i've done numerous grain to grain transferes in a still air box...

as for agar and LC... i just dont have the supplies.. but anything is possible.

and as far as my experience...

i still consider myself a noob. being that there are a lot of things i havent experienced.. but if you can grow a cube, you can manage a g2g im sure.

use good gloves, rub them down with alc. use a medical grade face mask just incase you breath too hard, and be quick, but at the same time try not to stir up too much air. sweet, have fun. good luck.

you can easily make 1 quart of grain into about 8-10 more quarts. i always keep one of my fully colonized grain jars aside for g2g.


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Offlineshevanel
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Re: grain to grain, liquid culture, agar, discuss. [Re: This guy]
    #8054070 - 02/21/08 09:28 PM (15 years, 11 months ago)

Quote:

This guy said:
i've done numerous grain to grain transferes in a still air box...

as for agar and LC... i just dont have the supplies.. but anything is possible.

and as far as my experience...

i still consider myself a noob. being that there are a lot of things i havent experienced.. but if you can grow a cube, you can manage a g2g im sure.

use good gloves, rub them down with alc. use a medical grade face mask just incase you breath too hard, and be quick, but at the same time try not to stir up too much air. sweet, have fun. good luck.

you can easily make 1 quart of grain into about 8-10 more quarts. i always keep one of my fully colonized grain jars aside for g2g.




2 days ago I attemted a g2g from 1 pint of wbs into 2 quarts of wbs, the growth has spread, but surely too early to tell if they'll survive. With the remaing 1/2 pint I spawned it into a quarts of coir/coff and the entire surface area of the coir is covered is very fluffy mycelium... I wonder why people can dump various quarts of colonized myc inopen air into pasturized hpoo or coir without using a glove box and succeed, but doing the same thing into a jar of seeds requires using a box, wearing gloves/facemask, wiping down with alcohol and getting p-noid about air movement, all of which I do.


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Invisibleblood4blood
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Re: grain to grain, liquid culture, agar, discuss. [Re: shevanel]
    #8054103 - 02/21/08 09:33 PM (15 years, 11 months ago)

when your doing g2g's your dumping sterile grains into sterile grains that need to stay sterile. there is no frequent air exchange whitch means it would be very easy for a contam to gain a foothold. when your dumping colonized grains into a sub and mixing it you no longer have a stagnat warm atmosphere which contams thrive in


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InvisibleThis guy
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Re: grain to grain, liquid culture, agar, discuss. [Re: shevanel]
    #8054109 - 02/21/08 09:33 PM (15 years, 11 months ago)

eh i do it just to be safe.. im sure if you have a very clean place you can do it in open air.. i case my bulk in open air.. but then again we're talking about fresh, uncolonized WBS, vs. fully colonized bulk.

uncolonized bird seed or grain is way more vulnerable to open air contams then anything that is fully colonised.


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InvisibleFooMan
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Re: grain to grain, liquid culture, agar, discuss. [Re: shevanel]
    #8054113 - 02/21/08 09:34 PM (15 years, 11 months ago)

You can definitely do all 3 methods with only a glovebox. Which one you use depends on your goals. With G2G you can expand your myc fast, but it's not good for long-term use due to senescense after a few transfers. LC's are good for generating lots of myc, which can also be transferred to more LC solution, but it has a limited shelf life and can also suffer from senescense with repeated transfers. Agar is best if you're goal is preserving a particular culture for long periods of time.

Spore prints will definitely last forever though, so make sure you take prints that you can always go back to.


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Offlineshevanel
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Re: grain to grain, liquid culture, agar, discuss. [Re: FooMan]
    #8054167 - 02/21/08 09:41 PM (15 years, 11 months ago)

ahh, all good info from everyone. So, no matter what, I'll always need to start from spore syringes at some point? I see.

From just reading the few responses I've recieved I already understand more about what I've already read, need to read and the pro's and con's of each... I suppose their is a place for each situation and it's up to the grower to choose which is best for that particualr time in the grow..

This hobby will never get old..


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InvisibleFooMan
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Re: grain to grain, liquid culture, agar, discuss. [Re: shevanel]
    #8054308 - 02/21/08 10:02 PM (15 years, 11 months ago)

Quote:

shevanel said:
ahh, all good info from everyone. So, no matter what, I'll always need to start from spore syringes at some point? I see.





Not neccessarily. There have been cultures that were many years old that were used with success later, but once you are out of that culture, then yes, I guess prints it is.


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