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Invisibledumbfounded1600
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Registered: 07/29/07
Posts: 2,624
Having Some Truble Understanding...Transfering
    #8053065 - 02/21/08 06:17 PM (15 years, 11 months ago)

Say you want to keep a culture in slant tubes for long period of time. I know to put Glad Cling Wrap around the test tube for FAE Exchange. Ok what I'm having trouble understand is after 2 years you take a piece out and put make a new one right? Right, after that will it start to degrade? Meaning will it not be as good as young healthy mycelium and once you take that piece if that's what the one you want to transfer onto another petri dish will it be "The old shitty not as outgoing and happy mycelium like young mycelium would be.?" and continue to produce shitty mycelium because that was basically the seed of it's roots correct?


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Offline1Hypermycosis1
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Registered: 02/05/08
Posts: 21
Loc: U.S.A.
Last seen: 15 years, 9 months
Re: Having Some Truble Understanding...Transfering [Re: dumbfounded1600]
    #8053326 - 02/21/08 07:11 PM (15 years, 11 months ago)

It depends on how much the mycelium has grown. If you try to make a culture from some mycelium that has gone through 10 grain to grain transfers then it will be old and degraded from all the cell divisions. If you use mycelium that has only gone through some petris(as in strain isolation) it will still be young and healthy. Think of a cultures age as the amount of cell divisions it has gone through.


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