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donki
Stranger
Registered: 02/21/08
Posts: 5
Last seen: 15 years, 11 months
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how much spore print for 1 syringe?
#8052517 - 02/21/08 04:11 PM (15 years, 11 months ago) |
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If one had an entire spore print, how much of that would be required for one syringe? (Assuming he is making eight double sized cakes) And also how careful must you be when dividing/cutting the print?
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skattman1982
Stranger


Registered: 09/18/07
Posts: 259
Last seen: 7 years, 11 months
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Re: how much spore print for 1 syringe? [Re: donki]
#8052529 - 02/21/08 04:13 PM (15 years, 11 months ago) |
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you need very little print depending on the size and density of the print. with a 1 inch print i rarely even come close to using 1/4 of the print itself. use more if you like and you will likely germinate quicker. it's up to you.
i try and get as much out of each print as possible. so one print would give me about four syringes.
-------------------- They lie about marijuana. Tell you pot-smoking makes you unmotivated. Lie! When you're high, you can do everything you normally do, just as well. You just realize that it's not worth the fucking effort. There is a difference.-Bill Hicks- by the way, everything i write is for entertainment purposes and is purely fictional. do not construe these written words with the author having any knowledge of actual persons, places, events, or actions.
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Cheesekiller
Mad Scientist



Registered: 11/08/04
Posts: 485
Loc: Central NY
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Re: how much spore print for 1 syringe? [Re: skattman1982]
#8052575 - 02/21/08 04:24 PM (15 years, 11 months ago) |
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First time making one?
Once I have lots of prints I use much more than you have 2. Most of my new prints are about 3in wide, so I use that for two 20cc syringes, and knock up 7 qt WBS jars with that.
They colonize real quick, like 5-7 days for full col. If you only have one then you might want to split a 3/4 in print in two 10CC syringes. Its up to you because there are millions ( I may be exagurating here) of spores in that 3/4 in print.
Experiment, but don't put just enough, more won't hurt.
-CK
-------------------- Bulk grower with "some" success. Cloning Machine Nice Lids A few pics of my DT setup
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donki
Stranger
Registered: 02/21/08
Posts: 5
Last seen: 15 years, 11 months
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Re: how much spore print for 1 syringe? [Re: Cheesekiller]
#8052598 - 02/21/08 04:29 PM (15 years, 11 months ago) |
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How careful must one be when splitting the print? Could one literally take the print out of the ziploc and cut it with scissors?
Also will bottled water work for the syringe water?
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blood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
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Re: how much spore print for 1 syringe? [Re: donki]
#8052784 - 02/21/08 05:09 PM (15 years, 11 months ago) |
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Quote:
donki said: How careful must one be when splitting the print? Could one literally take the print out of the ziploc and cut it with scissors?
Also will bottled water work for the syringe water?
you really need to do some reading. making syringes isnt exactly for beginners. you must be very sterile in your process.
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blood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
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Re: how much spore print for 1 syringe? [Re: blood4blood]
#8052811 - 02/21/08 05:17 PM (15 years, 11 months ago) |
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Magash
Da Bud Guru



Registered: 07/25/02
Posts: 5,876
Loc: Near Hilo
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Re: how much spore print for 1 syringe? [Re: donki]
#8052947 - 02/21/08 05:52 PM (15 years, 11 months ago) |
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When you only have a few prints you can get 8 decent syringes depending on the size and how dark the print is. Once you start taking your own prints and have a ton of em it's easier to just stick one in each syringe. (This is just a me thing)
-------------------- All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?
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donki
Stranger
Registered: 02/21/08
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Last seen: 15 years, 11 months
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Re: how much spore print for 1 syringe? [Re: blood4blood]
#8052956 - 02/21/08 05:54 PM (15 years, 11 months ago) |
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so while the spores are being "rehydrated" in the syringe for 12 hours, where should one store the syringe, or does it matter?
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Cheesekiller
Mad Scientist



Registered: 11/08/04
Posts: 485
Loc: Central NY
Last seen: 1 month, 1 day
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Re: how much spore print for 1 syringe? [Re: donki]
#8053006 - 02/21/08 06:06 PM (15 years, 11 months ago) |
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I put mine in a ziplock bag in my cabinet with my caned veggies and dry stuff. Only really cause its right next to where I will knock up the jars. Remember the spores will be most ripe 1 day from creation. Then they are really ready to start up your jars.
They will last but why wait, time it so you have sterile jars ready for getting knocked up!!
-CK
-------------------- Bulk grower with "some" success. Cloning Machine Nice Lids A few pics of my DT setup
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donki
Stranger
Registered: 02/21/08
Posts: 5
Last seen: 15 years, 11 months
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Re: how much spore print for 1 syringe? [Re: Cheesekiller]
#8053281 - 02/21/08 07:01 PM (15 years, 11 months ago) |
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how long do the jars stay sterile for after they have been cooled and before inoculation?
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donki
Stranger
Registered: 02/21/08
Posts: 5
Last seen: 15 years, 11 months
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Re: how much spore print for 1 syringe? [Re: donki]
#8057279 - 02/22/08 04:42 PM (15 years, 11 months ago) |
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?
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MayorMcCheese
Mayor



Registered: 11/10/07
Posts: 390
Last seen: 8 years, 2 months
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Re: how much spore print for 1 syringe? [Re: donki]
#8057614 - 02/22/08 05:54 PM (15 years, 11 months ago) |
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forever?
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Frost000
Badfish

Registered: 01/03/08
Posts: 236
Last seen: 10 years, 11 months
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Re: how much spore print for 1 syringe? [Re: MayorMcCheese]
#8058380 - 02/22/08 08:58 PM (15 years, 11 months ago) |
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That all depends. Are you using Pf tek, grains...?
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dumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
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Re: how much spore print for 1 syringe? [Re: Frost000]
#8058673 - 02/22/08 10:31 PM (15 years, 11 months ago) |
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Darker syringes will result in more contamination problems. There is always going to be a certain percentage of contaminant spores on any print. Therefore, if you use more spores, you get more contaminants. In addition, when you use a massive spore inoculation, you force the mycelium to spend a lot of time and energy combining all the genetics into a common network. Many growers think that more spores results in faster colonization. What it results in is the substrate turning 'white' faster, but there is no evidence at all the project will actually fruit sooner. I'm a firm believer in using the minimum amount of spores necessary to achieve a crop. Clear(to the naked eye) syringes perform better than dark syringes. Nobody can see individual spores with their eyeballs. What you see is clusters of a few thousand that are stuck together. The spores in the center of these clusters are locked out of the moisture in the jars, so they rarely even get a chance to germinate RR
This was not my post. I do highly agree as too many sub strains in a cake or jar will increase the chances of contaminated spores.
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legallyhomeless
mooch


Registered: 07/01/05
Posts: 4,051
Loc: EZRA for the Refuge
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Re: how much spore print for 1 syringe? [Re: dumbfounded1600]
#8066187 - 02/24/08 08:31 PM (15 years, 11 months ago) |
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I make a syringe and use it right away. 1 print = 2-3 syringes.
-------------------- MY TRADE LIST!!! FULLY AUTOMATIC!! 12-Pot Multi Grow Hydroponic System for Trade.
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flavoraid
now with twicethe ketamine andopiates!

Registered: 12/05/07
Posts: 1,678
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Re: how much spore print for 1 syringe? [Re: Magash]
#8174865 - 03/21/08 03:22 AM (15 years, 10 months ago) |
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Quote:
Magash said: When you only have a few prints you can get 8 decent syringes depending on the size and how dark the print is. Once you start taking your own prints and have a ton of em it's easier to just stick one in each syringe. (This is just a me thing)
Unless you print your prints in a very clean environment and your grow area is relatively dust free (not saying yours isn't) darker isn't necessarily better, as all prints have some level of contamination. If you make your prints on foil or glass slides infront of a flowhood or better yet with a screen between the mushroom and printing surface go nuts with how dark you make your syringes.
Otherwise you may be introducing excessive amounts of contamination, RR said metabolites would also be plentiful so this issue is sort of balanced out - depending on how clean your printing technique is.
-------------------- coda said: imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.
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Blueroots
Evonaut


Registered: 03/06/08
Posts: 3
Loc: over there
Last seen: 12 years, 7 months
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Re: how much spore print for 1 syringe? [Re: flavoraid]
#8175630 - 03/21/08 11:52 AM (15 years, 10 months ago) |
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As a rule of thumb liquid in the syringe should be lightly greyish violet if put against a white paper. A 1/2"x1/2" print is quite enough for a 10cc. At least thats my FOAF thinks optimal.
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shoeareyou
Stranger


Registered: 01/07/07
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Last seen: 9 years, 1 month
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Re: how much spore print for 1 syringe? [Re: Magash]
#8175745 - 03/21/08 12:32 PM (15 years, 10 months ago) |
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hahahahah WHAT a fucking clutch! Nice syringes mate I aspire to that. They're all really dark aswell (I know vendors say it doesn't make a difference, but spores are dark, therefor dark syringes must have more spores...)
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Roadkill
Retired Shroomery Mod



Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: how much spore print for 1 syringe? [Re: donki]
#8175864 - 03/21/08 01:07 PM (15 years, 10 months ago) |
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Quote:
donki said:
If one had an entire spore print, how much of that would be required for one syringe? (Assuming he is making eight double sized cakes)
And also how careful must you be when dividing/cutting the print?
If you have a semi nice dark print...
using a 1/4" by 1/4" square area from a print will make (1) syringe.
If the print isn't semi dark use a little more.
I suggest scraping the spores off the spore print using a sterile tool of some sort.
I like to use a sterile Exacto knife.
tc
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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Roadkill
Retired Shroomery Mod



Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: how much spore print for 1 syringe? [Re: donki]
#8175906 - 03/21/08 01:16 PM (15 years, 10 months ago) |
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Quote:
donki said:
so while the spores are being "rehydrated" in the syringe for 12 hours, where should one store the syringe, or does it matter?
how long do the jars stay sterile for after they have been cooled and before inoculation?
I put my syringes in a ziplock bag or a container to keep contams that float in the air, from falling on them.
If storing a syringe for a long period of time... I put them in a ziplock bag, then into a container... then place them in my frig. I have syringes that are still viable, that are almost 6 years old.
Your jars will be good for a few weeks to a month, or so. The main problem that you will run into is that the substrate in the jar will dry out over time.
tc
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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