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Offlineequalizer
Mr
Registered: 11/29/07
Posts: 10
Last seen: 15 years, 24 days
No mycelium growth so far...
    #8050373 - 02/21/08 01:45 AM (15 years, 11 months ago)

Tomorrow will be a week since inoculation and I am yet to see any growth on any of my jars. I am using rye as the substrate. To prepare the rye I soaked it for 24 hours, then strained the water. Pressure cooked the jars for 1 hour at 15psi.

I've been reading the forums to see how long the wait could possibly be and what could have caused the time delay. I'm not sure the rye dried well before being pressure cooked, don't know if that would cause anything. When inoculating I used approximately 2cc's of spores per jar.

There are ten jars total and as far as I can tell there is no growth. 5 jars are one strain and 5 are another. I read somewhere that I should just be patient and as long as there is no sign of contamination to just give it time.

The setup of my incubation chamber is pretty good. I have a fridge with a heater inside and the temperatures are kept between 82 and 85 degrees through out the day.

Any input would be appreciated and let me know if I should be worried or just need to work on my patience.

Thanks in advance


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Offlinedl46
Stranger

Registered: 02/01/08
Posts: 19
Last seen: 15 years, 10 months
Re: No mycelium growth so far... [Re: equalizer]
    #8050478 - 02/21/08 03:40 AM (15 years, 11 months ago)

Does the jar smell at all? Also, where did you get your spores from?


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Offlineequalizer
Mr
Registered: 11/29/07
Posts: 10
Last seen: 15 years, 24 days
Re: No mycelium growth so far... [Re: dl46]
    #8050481 - 02/21/08 03:46 AM (15 years, 11 months ago)

This is the first grow that I have done so I am not one to say that something doesn't smell bad, but the smell is pretty much the same rye smell as when it came out of the PC. I got the Pink Buffalo and Alacabenzie strains from spores101.


Edited by equalizer (02/21/08 04:05 AM)


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Invisiblexile
I'm a teapot User Gallery

Registered: 10/04/07
Posts: 63
Re: No mycelium growth so far... [Re: equalizer]
    #8050577 - 02/21/08 06:07 AM (15 years, 11 months ago)

How long did you let them cool after being PC'd? If you didnt wait the full 12-24 hours they could have been too hot for the spores.

Also, i waited 2 weeks before I showed growth in my last batch of rye and they did just fine. Did you inject in the center? Shake it up after?


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Invisibleshroomiin
unprofound


Registered: 12/24/07
Posts: 2,470
Loc: Zone 6 Flag
Re: No mycelium growth so far... [Re: xile]
    #8051001 - 02/21/08 09:10 AM (15 years, 11 months ago)

I didn't see much growth either in my jars until after a week or so. It seems that when i kept them warmer (85-86*f) the mycelluim growth would go faster. But when they were left in a room at about 70-something they wouldn't grow much at all.


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Offlineendepth
The world remains ever the same.


Registered: 12/25/07
Posts: 647
Loc: Cloud 9
Last seen: 3 years, 6 months
Re: No mycelium growth so far... [Re: shroomiin]
    #8051366 - 02/21/08 10:38 AM (15 years, 11 months ago)

Patience! a week is nothing... Start to worry after 14 days or so.


--------------------
Being normal is nothing to aspire to. It is something to get away from.



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Offlineequalizer
Mr
Registered: 11/29/07
Posts: 10
Last seen: 15 years, 24 days
Re: No mycelium growth so far... [Re: endepth]
    #8051691 - 02/21/08 12:34 PM (15 years, 11 months ago)

Thank you for the replies. Just to answer some of the questions/comments...

The jars were left to cool in the PC over night and were then inoculated the next afternoon. When inoculating I did about 1cc on each side of the jar and then gave it a shake.

I guess for now I'll play the waiting game, thanks for the input.


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OfflineRoseM
Devil's Advocate
Female User Gallery


Registered: 09/24/03
Posts: 22,518
Loc: Mod not God Flag
Last seen: 1 year, 6 months
Trusted Cultivator
Re: No mycelium growth so far... [Re: equalizer]
    #8051707 - 02/21/08 12:38 PM (15 years, 11 months ago)

Did you simmer your grains, add water to the jars before pcing them, or did you just soak for 24 and put them in the PC?

Can you shake your grains?

Are they stuck together?


--------------------
Fiddlesticks.



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Offlineequalizer
Mr
Registered: 11/29/07
Posts: 10
Last seen: 15 years, 24 days
Re: No mycelium growth so far... [Re: Rose]
    #8058000 - 02/22/08 07:28 PM (15 years, 11 months ago)

Unfortunately I didn't simmer the grains. The guide I was using did not mention it and until I started looking into what could have gone wrong I hadn't heard of it.

Before pressure cooking I had soaked the grain for 24 hours, then strained the water. There was no water in the jars when they were PCed.

Now, the jars are fairly dry and easy to shake. Takes a few shakes to break everything up, but overall is not a problem.


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Invisibledumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
Re: No mycelium growth so far... [Re: equalizer]
    #8058043 - 02/22/08 07:38 PM (15 years, 11 months ago)

No need to simmer but..
If you failed to dry the grains properly and there still wet inside the jar. Incubating at those temps will indeed increase your chances of contamination. There's no need to use an incubator! If you feel fine in with a t-shirt on inside your home it's fine. 75-78F is perfect, they'll just colonize a few days later and they won't sweat the mycelium.
If your house is cold don't use an incubator. Use a space heater as this will heat up the entire area around your jars and will not sweat your jar. Incubation causes condensation inside each jar which
1. Fucks up your water content.
2. Condensation droplets fall to the bottom creating a coin in thickness pool of water and INCREASING your chance of contamination. You do not want this.


Don't shake everytime you see mycelium THIS damages mycelium kernals. Shake at 25% and then stop. Shake and break up at 100% colonization. I ask
What are you doing for gas exchange for your jars?


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Offlineequalizer
Mr
Registered: 11/29/07
Posts: 10
Last seen: 15 years, 24 days
Re: No mycelium growth so far... [Re: dumbfounded1600]
    #8058073 - 02/22/08 07:45 PM (15 years, 11 months ago)

Well, my jars are in a decent size mini fridge at the moment. The jars have breathing holes that I cut into them on the top and then have a layer of tyvek. There are two cpu fans that are constantly running inside the fridge to distribute heat and then i fan the door once or twice a day. Don't have any constant air exchange however.


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Invisibledumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
Re: No mycelium growth so far... [Re: equalizer]
    #8058080 - 02/22/08 07:48 PM (15 years, 11 months ago)

Take them out of the fan running incubator.
1. The fan is just stirring up turbulence of any contaminated spores that may have been in there.
2. Take it out of the incubator and put them on a shelf at room temperature.


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Offlineequalizer
Mr
Registered: 11/29/07
Posts: 10
Last seen: 15 years, 24 days
Re: No mycelium growth so far... [Re: dumbfounded1600]
    #8058086 - 02/22/08 07:49 PM (15 years, 11 months ago)

Alright, I'll give it a try, thanks for the advice


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