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ofzeroconcern
Mindflayer

Registered: 04/13/06
Posts: 226
Loc: Aurora
Last seen: 8 years, 6 months
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Almost 100% colonization, some general questions about dunking/fruiting/etc.
#8045498 - 02/19/08 11:11 PM (15 years, 11 months ago) |
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My jars are at about 99%, only a small spot in the middle on the very bottoms of the jars are uncolonized. Hopefully this is not CO2 buildup, otherwise I'd have to turn them upside-down. Anyway, gonna give them another week or two to consolidate.
So to the questions. How important would you people say it is to dunk these (water, refrigerated, 24 hours) in separate containers? How many of you dunk separately? I was thinking of just dunking them all in my pressure cooker and putting it in the fridge. Or maybe a smaller pot or something. But I figure if a contam gets in the water somehow, then ALL the cakes are contaminated. How much of a risk is this?
Then I'll roll, probably not gonna cook the verm first unless I get flooded with people telling me I should. Most people say it's not necessary. Same with perlite, especially since I will be putting the cakes on foil squares.
My FC already has tons of holes in it, decided to not go with tyvek or any other kind of filler for the holes, even though it seems to be a 50/50 argument. Got a calibrated analogue hygrometer in there. Gonna elevate it an inch or two as it sits.
I intend to give it 12 hours of regular room light a day, not sunlight, I just got a ceiling lamp, the light isn't close or direct or anything. Gonna keep it between 60 and 80 F, mist and fan 3 times a day, or more misting if it's needed to keep the humidity as high as possible.
Oh and on misting, do I mist the cakes directly or just the perlite or the walls or what?
But yeah that's my plan basically. Anything I should change? Should I buy a special light? Should I change or alter anything? Any input appreciated, just double checking before I move on, if this grow is successful, it will be my first.
Thanks.
-------------------- "Who can see the future? Those who create it."
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miles420
Shrooms :)


Registered: 02/01/08
Posts: 39
Last seen: 9 years, 9 months
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Re: Almost 100% colonization, some general questions about dunking/fruiting/etc. [Re: ofzeroconcern]
#8045704 - 02/19/08 11:48 PM (15 years, 11 months ago) |
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dont mist the cakes
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woefuljungle
here to gain andshare knowlege


Registered: 02/05/08
Posts: 172
Loc: us
Last seen: 15 years, 2 months
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Re: Almost 100% colonization, some general questions about dunking/fruiting/etc. [Re: miles420]
#8045740 - 02/19/08 11:55 PM (15 years, 11 months ago) |
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i heard mist the cakes but mist up in the air and let if fall...but if you dont mist the cakes where would u mist?
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xaxphaanes
Mycologist



Registered: 08/08/05
Posts: 2,988
Last seen: 1 year, 5 months
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Re: Almost 100% colonization, some general questions about dunking/fruiting/etc. [Re: woefuljungle]
#8045748 - 02/19/08 11:56 PM (15 years, 11 months ago) |
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Quote:
i heard mist the cakes but mist up in the air and let if fall..
that is correct you have to mist cakes or else the surface will dry out and you wont make a very friendly environment for primordia development.
-------------------- "Anything i say is fictional" what you should look for in manure
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dumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
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Re: Almost 100% colonization, some general questions about dunking/fruiting/etc. [Re: xaxphaanes]
#8045782 - 02/20/08 12:07 AM (15 years, 11 months ago) |
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You can damage the hyphae knots if you mist directly...Mist from a good 2 feet away so the water vapor hits it
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atomicblue
Stranger



Registered: 11/09/07
Posts: 206
Loc: UP
Last seen: 14 years, 2 months
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Re: Almost 100% colonization, some general questions about dunking/fruiting/etc. [Re: dumbfounded1600]
#8045836 - 02/20/08 12:37 AM (15 years, 11 months ago) |
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Two feet!?!? Your gonna need a garden hose...Oh forget it. Too technical. Maybe you could just make sure it's a fine mist so you don't blow your knots off as suggested above. No one looks too cool with their knots blown off. Most sprayers are adjustable. Sounds fun your almost there! Do mist your cakes as needed all life needs agua and these guys love it!
Edited by atomicblue (02/20/08 12:50 AM)
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MuShQuEsT
Night Wanderer



Registered: 12/31/07
Posts: 68
Loc: North Western Oregon
Last seen: 15 years, 1 month
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Re: Almost 100% colonization, some general questions about dunking/fruiting/etc. [Re: atomicblue]
#8046610 - 02/20/08 10:18 AM (15 years, 11 months ago) |
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You need to mist even if you dunked?
I have mine in an automated chamber and I dunked so I figured i wouldn't need to mist?
-------------------- Professor Farnsworth: For example, if you killed your grandfather, you'd cease to exist! Fry: But existing is basically all I do! -Futurama "Roswell that ends well"
Edited by MuShQuEsT (02/20/08 10:19 AM)
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Neobean
Adept Mycologist


Registered: 10/07/01
Posts: 975
Loc: Canada
Last seen: 3 years, 8 months
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Re: Almost 100% colonization, some general questions about dunking/fruiting/etc. [Re: MuShQuEsT]
#8046625 - 02/20/08 10:24 AM (15 years, 11 months ago) |
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Quote:
MuShQuEsT said: You need to mist even if you dunked?
I have mine in an automated chamber and I dunked so I figured i wouldn't need to mist?
Dunking is to rehydrate the substrate within. Misting is to keep the surface humid. If you also rolled, the verm will protect some of your cake as you mist. Fine mist is best, but if you have no other option, mist heavy, its still preferable to keep the surface humid. If you automated chamber provides this, don't worry about it
-------------------- If y0u want s0meting gr0wn right, y0u g0tta gr0w it y0urself!!!
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ofzeroconcern
Mindflayer

Registered: 04/13/06
Posts: 226
Loc: Aurora
Last seen: 8 years, 6 months
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Re: Almost 100% colonization, some general questions about dunking/fruiting/etc. [Re: Neobean]
#8046898 - 02/20/08 11:36 AM (15 years, 11 months ago) |
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Thanks guys. What about light? Would just 12 hours of normal room light be good enough? Or should I get a special florescent light or something? Or should I move my lamp closer, or turn it around so the build is in direct view of the FC?
-------------------- "Who can see the future? Those who create it."
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Bungalow Bill
Happiness is aWarm Gun


Registered: 02/14/08
Posts: 69
Loc: Peking
Last seen: 15 years, 9 months
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Re: Almost 100% colonization, some general questions about dunking/fruiting/etc. [Re: ofzeroconcern]
#8046939 - 02/20/08 11:46 AM (15 years, 11 months ago) |
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Give them 12 hours of 6500 Kelvin color temp, CFL.
www.bulbs.com
-------------------- The fruit shall set you free.
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Neobean
Adept Mycologist


Registered: 10/07/01
Posts: 975
Loc: Canada
Last seen: 3 years, 8 months
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Re: Almost 100% colonization, some general questions about dunking/fruiting/etc. [Re: ofzeroconcern]
#8047054 - 02/20/08 12:18 PM (15 years, 11 months ago) |
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Quote:
ofzeroconcern said: Thanks guys. What about light? Would just 12 hours of normal room light be good enough? Or should I get a special florescent light or something? Or should I move my lamp closer, or turn it around so the build is in direct view of the FC?
Regular day cycle light is good. Just no direct sunlight You wouldnt wanna dry your shit out...
-------------------- If y0u want s0meting gr0wn right, y0u g0tta gr0w it y0urself!!!
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ofzeroconcern
Mindflayer

Registered: 04/13/06
Posts: 226
Loc: Aurora
Last seen: 8 years, 6 months
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Re: Almost 100% colonization, some general questions about dunking/fruiting/etc. [Re: Neobean]
#8049359 - 02/20/08 09:22 PM (15 years, 11 months ago) |
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Oh and no one really replied to my whole should I dunk separately thing. So how much of a contam risk is there from dunking all my cakes in the same vessel?
-------------------- "Who can see the future? Those who create it."
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thashaman
Biophilosopher

Registered: 11/09/04
Posts: 161
Last seen: 13 years, 10 months
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Re: Almost 100% colonization, some general questions about dunking/fruiting/etc. [Re: ofzeroconcern]
#8049492 - 02/20/08 09:39 PM (15 years, 11 months ago) |
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Dunk separately for the following reason...
Chances are if you dunked them all together, assuming you have competent sterile technique/cleanliness, you probably would NOT get any contams (fully colonized cakes are fairly resistant to contams...Mycelium is capable of secreting thier own defensins.). However, if you have any doubts about your sterile technique/cleanliness whatsoever, dunk separately. If you DO by mistake have a dirty container or a single bacterial/mold endospore that decides to proliferate in your dunking chamber. Your entire crop is destroyed...
If you dunk separately, then the loss of one or two cakes just sucks, but it isn't catastrophic.
I am quite confident in my technique as I have well over 10 grows under my belt...but I still consider it a big risk and therefore I dunk separately.
Here is what I suggest. Dunk the cakes in the containers you colonized in. That is to say, fill the containers with water, don't even take the cakes out at the end of colonization, just simply spray the air with OUST/Lysol, some sort of disinfectant, dump out your top layer of vermiculite, and fill each jar with water. Put the lids back on and place in the fridge for 24 hours. Then roll in fresh vermiculite and place in your FC. Hope this helps.
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ofzeroconcern
Mindflayer

Registered: 04/13/06
Posts: 226
Loc: Aurora
Last seen: 8 years, 6 months
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Re: Almost 100% colonization, some general questions about dunking/fruiting/etc. [Re: thashaman]
#8049678 - 02/20/08 10:15 PM (15 years, 11 months ago) |
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Hm, I was actually thinking of using the jars to dunk, but wasn't sure if it would be enough water. I did a search for it and didn't find any info or indication that anyone else has done it, so just decided to not go with it. But if you tried it and it works, then I guess I'll go for it.
So yeah, I remove the top layer of verm, but I don't rinse off the cakes beforehand? I know you said not to, but what are the pros/cons of rinsing before dunking?
-------------------- "Who can see the future? Those who create it."
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thashaman
Biophilosopher

Registered: 11/09/04
Posts: 161
Last seen: 13 years, 10 months
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Re: Almost 100% colonization, some general questions about dunking/fruiting/etc. [Re: ofzeroconcern]
#8049704 - 02/20/08 10:21 PM (15 years, 11 months ago) |
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No need to mist. There are no added benefits. Think about it like this...Once those jars are full of H20, they are getting a constant misting for 24 hours. So a mist or two really doesn't make a bit of difference. Also, if you take the cakes out of the jars to mist, you are just adding unnecessary exposure to airborne contams...although hopefully you would have OUSTed the air before taking them out.
I have used this method on 9 of my 10 grows. Works like a charm, and minimizes exposure to contams. Making sure that the jar are full of water is PLENTY of water for a 24 hour dunk....CHEERS!
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just me
Friend



Registered: 07/01/07
Posts: 9,745
Loc: IL/MO/FL/TX/HI/OR
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Re: Almost 100% colonization, some general questions about dunking/fruiting/etc. [Re: thashaman]
#8049989 - 02/20/08 11:24 PM (15 years, 11 months ago) |
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dunking in the jars works really well. the only thing, sometimes the cakes shrink in the jar, so maybe submerse the cake with a ball of foil or somethin similar and sterile in between the cake and the lid.
i mist my cakes...HEAVILY, up until pinning, then i continue to mist the walls and perlite to heep the RH% up, and if i have a cake where i can mist inbetween mushies i still spay in between them...they just seem to drink that fresh distilled water up...yum yum
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  -------------------------------------------------- -pEaCeLoVeGoDbLeSs- "The Downfall of Mankind; is Believing He Has Limitations."
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BlargIAmDead
Shroom Samurai




Registered: 11/04/07
Posts: 550
Last seen: 14 years, 11 months
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Re: Almost 100% colonization, some general questions about dunking/fruiting/etc. [Re: just me]
#8050032 - 02/20/08 11:35 PM (15 years, 11 months ago) |
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Alright shaman I think we need to work on our reading skills. No you don't want to mist when you're dunking them. Yes you DO want to mist when you have them in your FC. Mist and fan about 5-7 times a day. You can go easy on the misting if there are water droplets everywhere. I had some of my caps start to rot because I kept them TOO wet. And you can just dunk them all in the same pot. Your FC isn't sterile by any stretch of the imagination and your dunking pot should be clean but you don't have to like autoclave it.
Here's what you do: Take a large pot, put water in it, put in cakes, put in plate with weight if needed. Wait 24 hours. Done.
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xaxphaanes
Mycologist



Registered: 08/08/05
Posts: 2,988
Last seen: 1 year, 5 months
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Re: Almost 100% colonization, some general questions about dunking/fruiting/etc. [Re: BlargIAmDead]
#8050282 - 02/21/08 12:46 AM (15 years, 11 months ago) |
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lol mist 5-7 times a day??????? hope you dont have a life or a job lolz
-------------------- "Anything i say is fictional" what you should look for in manure
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thashaman
Biophilosopher

Registered: 11/09/04
Posts: 161
Last seen: 13 years, 10 months
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Re: Almost 100% colonization, some general questions about dunking/fruiting/etc. [Re: BlargIAmDead]
#8050988 - 02/21/08 09:08 AM (15 years, 11 months ago) |
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Quote:
BlargIAmDead said: Alright shaman I think we need to work on our reading skills...
Are you stoned...or just inattentive? I never said he didn't need to mist EVENTUALLY. I was simply referring to misting right before the dunk. Had you read his original question...the one imediately before my post, you would have understood. Also misting 5-7 times a day is very unceccsary. At 100% humidity, there is so much water vapor in the air, that it might actually collect on the surface of the cakes. The mycelium can definately utilize this moisture. I would recommend misting once a day. I understand everyone is different and if it worked for you BlargIAmDead, then keep doing it. But as for the original question, you do not need to mist right before dunking. Additionally, if you overnight dunk between each flush, your misting requirements go down even further, especially if you rolled in vermiculite.
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ofzeroconcern
Mindflayer

Registered: 04/13/06
Posts: 226
Loc: Aurora
Last seen: 8 years, 6 months
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Re: Almost 100% colonization, some general questions about dunking/fruiting/etc. [Re: thashaman]
#8051761 - 02/21/08 12:53 PM (15 years, 11 months ago) |
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I don't think I need to fan that many times a day, you should see how many holes I drilled in this thing. Some people say with holes, you don't need to fan AT ALL. I'm still going to though, just every time I mist, and I will mist between 1 - 4 times a day at FIRST, then from there, just check my hygrometer. If it needs more or less, then I'll figure it out from there.
As for misting before dunking in the jars, I meant to get the top layer of verm off and just to I guess remove anything that the eye can't see. I asked because in RR's videos, right before he dunks, he rinses them under running water from a faucet, then dunks them.
-------------------- "Who can see the future? Those who create it."
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